Acute and sub-acute toxicity of the aqueous extract from the stem bark of Tetrapleura tetrapteura Taub. (Fabaceae) in mice and rats

Dongmo, Olivette Laure Matafack; Epoh, Nadine Joissy; Tadjoua, Herve Tchoumbou; Yousuf, Sammer; Telefo, Phélix Bruno; Tapondjou, Léon Azefack; Choudhary, M. Iqbal

doi:10.1016/j.jep.2019.02.026

Cited by 26 publications

(13 citation statements)

References 19 publications

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“…Body weight changes are the basic parameter which indicates the health status of animals. However, abrupt variations in the body weight indicate the toxic nature of the chemicals or drugs (Dongmo et al, 2019). In this study, LA treatment and control group recorded a normal gain in body weight, indicating, LA does not cause any physiological alterations that affects the normal growth in the animals, which is in agreement with the previous study published by Clemente et al (2019).…”

Section: Discussionsupporting

confidence: 92%

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Khan

Aamir

Sisinthy

et al. 2020

PeerJ

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Background Lauric acid (LA), a common constituent of coconut oil, is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods The study was conducted in accordance with the Organization for Economic Co-operation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2,000 mg/kg body weight, respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results The study results showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion The current finding suggests that single oral administration of LA, even up to 2,000 mg/kg body weight, did not exhibit any signs of toxicity in SD rats; thus, it was safe to be used on disease models in animals.

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Section: Discussionsupporting

confidence: 92%

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Khan

Aamir

Sisinthy

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…However, abrupt variations in the body weight indicate the toxic nature of the chemicals or drugs (Dongmo et al 2019). In this study, LA treatment and control group recorded a normal gain in body weight, indicating, LA does not cause any physiological alterations that affects the normal growth in the animals, which is in agreement with the previous study published by (Clemente et al 2019).…”

Section: Discussionsupporting

confidence: 92%

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Sari¹

2020

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Background: Lauric acid (LA), a common constituent of coconut oil is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective: The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods: Study was conducted in accordance with the Organization for Economic Cooperation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2000 mg/kg body weight respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15 th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results: Study result showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion: Current finding suggests that single oral administration of LA, even up to 2000 mg/kg body weight did not exhibit any signs of toxicity in SD rats, thus, safe to be used on disease model in animals.

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“…Hence, the 150mg/kg of ethanolic extract of A. fruticosa leaves was selected as an optimum dose. This result was supported by the previous findings of Dongmo [20].…”

Section: Discussionsupporting

confidence: 91%

Assessment of anti-snake venom effects of Acalypha fruticosa leaves against Indian Saw-scaled viper by using envenomed Wistar albino rats

Malathi¹,

Duraikannu²,

Solaimuthu³

2019

IJSRBS

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Indian saw-scaled viper are one of the highly venomous snake found in Indian subcontinent and its venom has a wide variety of toxic compounds and it can able to causing severe envenoming characterized by severe pharmacological effects. The traditional knowledge on the Acalypha fruticosa plant was clearly states that this plant was used against the venomous bites and stings. In best of our knowledge, there were no any scientific reports to justify the anti-snake venom potential of this plant. The main objective of the present research work was to evaluate the anti-snake venom activity of A. fruticosa against Indian saw-scaled viper venom using envenomed animal model. The effects of ethanolic leaves extract of A. fruticosa on hematological parameters of experimental rats, saw-scaled viper venom induced liver toxicity, renal toxicity and blood metabolites was analyzed in experimental rats. In the present study, the ethanolic leaves extract of A. fruticosa leaves was inhibited the venom induced changes in hematological parameters. The plant extract was effectively decreased the saw-scaled viper venom induced liver toxicity and renal toxicity in experimental rats. The observed results clearly states that the A. fruticosa leaves has potent snati-snake venom activity.

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Acute and sub-acute toxicity of the aqueous extract from the stem bark of Tetrapleura tetrapteura Taub. (Fabaceae) in mice and rats

Cited by 26 publications

References 19 publications

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Assessment of anti-snake venom effects of Acalypha fruticosa leaves against Indian Saw-scaled viper by using envenomed Wistar albino rats

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