Acute toxicity of <i>Schizolobium parahyba</i> aqueous extract in mice

Mendes, Mirian Machado; Vale, Luis Henrique Ferreira do; Lucena, Malson N.; Vieira, Sâmela Alves Pereira Batista; Izidoro, Luíz Fernando Moreira; Robson, J. O.; Soares, Andreimar M.; Alcântara, Tânia Machado de; Hamaguchi, Amélia; Homsi-Brandeburgo, Maria Inês; Rodrigues, Veridiana M.

doi:10.1002/ptr.2956

Cited by 5 publications

(2 citation statements)

References 25 publications

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“…Escalating the dose of SP up to 2.0 mg/g did not induce serum creatinine (SCr) changes [43]. However, SCr is neither a sensitive nor a precise marker for evaluating the renal function in mice.…”

Section: Discussionmentioning

confidence: 99%

Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury

et al. 2014

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BackgroundVenom-induced acute kidney injury (AKI) is a frequent complication of Bothrops snakebite with relevant morbidity and mortality. The aim of this study was to assess the effects of Schizolobium parahyba (SP) extract, a natural medicine with presumed anti-Bothrops venom effects, in an experimental model of Bothrops jararaca venom (BV)-induced AKI.MethodologyGroups of 8 to 10 rats received infusions of 0.9% saline (control, C), SP 2 mg/kg, BV 0.25 mg/kg and BV immediately followed by SP (treatment, T) in the doses already described. After the respective infusions, animals were assessed for their glomerular filtration rate (GFR, inulin clearance), renal blood flow (RBF, Doppler), blood pressure (BP, intra-arterial transducer), renal vascular resistance (RVR), urinary osmolality (UO, freezing point), urinary neutrophil gelatinase-associated lipocalin (NGAL, enzyme-linked immunosorbent assay [ELISA]), lactate dehydrogenase (LDH, kinetic method), hematocrit (Hct, microhematocrit), fibrinogen (Fi, Klauss modified) and blinded renal histology (acute tubular necrosis score).Principal FindingsBV caused significant decreases in GFR, RBF, UO, HcT and Fi; significant increases in RVR, NGAL and LDH; and acute tubular necrosis. SP did not prevent these changes; instead, it caused a significant decrease in GFR when used alone.ConclusionSP administered simultaneously with BV, in an approximate 10∶1 concentration, did not prevent BV-induced AKI, hemolysis and fibrinogen consumption. SP used alone caused a decrease in GFR.

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Section: Discussionmentioning

confidence: 99%

Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury

et al. 2014

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“…[13] Mice were divided into nine groups of eight mice each to assess the toxicity of the HPE. CC and AT extracts were administered by i.p.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory potential of methanolic extracts ofAristolochia tagalaandCurcuma caesiaon hepatocellular carcinoma induced by diethylnitrosamine in BALB/c mice

Hadem¹,

Sharan²,

Kma³

2014

J Carcinog

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Context:Aristolochia tagala (AT) and Curcuma caesia (CC) have been used traditionally by local herbal practitioners for cancer treatment and as chief ingredients of many polyherbal formulations for various types of ailments. However, there is void in scientific study to evaluate their anti-cancer property.Aims:The aim of this study was to evaluate the anti-carcinogenic properties of the crude methanolic extracts of roots of AT and rhizomes of CC in BALB/c mice exposed to a hepatocarcinogen, diethylnitrosamine (DEN).Settings and Design:(I) Toxicity of herbal plant extracts (HPE); (II) Anticancer studies; (III) Histological studies; and (IV) Biochemical studies.Materials and Methods:To evaluate the effects of these two HPE either alone or following DEN exposure, serum transaminases (aspartate aminotransferase [AST], alanine aminotransferase [ALT]), alkaline phosphatase (ALP), and cancer marker enzyme acetylcholine esterase (AChE) were assayed in mice. In addition, histological study was also carried out under similar conditions. The antioxidant potentials of the HPE were evaluated by monitoring the activity of antioxidant enzymes and metabolites, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione (GSH).Statistical Analysis Used:Statistical analysis was performed by GraphPad Prism 6 Software using one-way analysis of variance followed by the Tukey's multiple comparisons test. Significance was set at P < 0.05.Results:Our findings show that DEN administration elevated AST, ALT, ALP, and AChE activities. CC or AT extracts attenuated the increased activities of these marker enzymes. The activities of antioxidant enzymes, which were decreased following DEN administration, were significantly increased in mice treated with CC or AT.Conclusions:The present study clearly documents anticarcinogenic and antioxidant properties of AT and CC in DEN-induced mouse liver cancer in vivo.

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