Acute Toxicity of Nitrite to Various Life Stages of the Amazon River Prawn, Macrobrachium amazonicum, Heller, 1862

Dutra, Fabrício Martins; Freire, Carolina A.; Vaz‐dos‐Santos, André Martins; Forneck, Sandra Carla; Brazão, Claudia Caramelo; Ballester, Eduardo Luís Cupertino

doi:10.1007/s00128-016-1932-2

Cited by 13 publications

(6 citation statements)

References 32 publications

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“…The LC 50 96h for isolated total ammonia and nitrite found in this study for M. amazonicum were lower than the values reported by Dutra et al. (2016a, 2016b), which were 36.59 mg/L total ammonia and 2.34 mg/L nitrite, respectively. Brazão et al.…”

Section: Resultscontrasting

confidence: 82%

“…Brazão et al (2021) found LC 50 96h values of 29.85 and 2.49 mg/L for total ammonia and nitrite, respectively. The variation in LC 50 96h values observed between the works may be related to the size of the animals (8.2 g in the present work) Dutra et al (2016aDutra et al ( , 2016b…”

mentioning

confidence: 53%

“…The concentrations chosen for the tests were based on the CL 50 96h determined by Dutra et al. (2016a, 2016b).…”

Section: Methodsmentioning

confidence: 99%

“…Although some studies on water quality already show safe lethal concentration and safety level data for ammonia or nitrite alone for the production of M. amazonicum (Dutra et al, 2016a(Dutra et al, , 2016b, little is known about the combined effect of these compounds (Brazão et al, 2021). When combined, ammonia and nitrite can present a synergistic, antagonistic or additive effect (Rand et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Dutra et al (2016aDutra et al ( , 2016b, which were 36.59 mg/L total ammonia and 2.34 mg/L nitrite, respectively.…”

mentioning

confidence: 99%

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Gill and haematological alterations in Macrobrachium amazonicum adults in acute exposure to ammonia and nitrite isolated and combined

Dutra

Juvenal

Forneck

et al. 2022

Aquaculture Fish & Fisheries

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The present study evaluated the isolated and combined effect of total ammonia and nitrite on Macrobrachium amazonicum adults through the median lethal concentration (LC5096h), histological analysis of gills and total and differential haemolymph cell counts. Prawns (n = 640; 9.8 ± 0.9 cm and 8.22 ± 1.29 g) were subjected to an entirely randomised design in a 4 × 4 factorial scheme. Individuals were exposed to total ammonia (0, 18, 36 and 72 mg/L) and nitrite (0, 1, 2 and 4 mg/L) alone and in combination. Interaction between the compounds was determined by summing the additivity of the substances (S) and by calculating the additivity index (AI). The LC5096h of isolated compounds was 27.98 mg/L for total ammonia and 1.87 mg/L for nitrite. Total ammonia and nitrite combined showed an LC5096h of 22.36 and 1.17 mg/L, respectively. The sum of additivity ranged from 2.70 to 1.42 and the AI indicated that the toxicity of the mixture was 1.70 to 0.42 times lower, indicating an antagonistic effect of the compounds. High total haemocyte counts (THC) were observed at the higher concentrations of both compounds at a shorter exposure time. Reduction in THC was observed in the treatments with a longer exposure time. Differential haemocyte counts were similar between treatments. Increases in the extent of regressive damage and organ index (Iorg) values occurred with increasing concentrations of isolated total ammonia and nitrite. Mild to moderate presence of progressive damage was observed at intermediate combined concentrations, which elevated the Iorg values. The interaction of total ammonia and nitrite promoted a reduction in mortality compared to treatment with the isolated compounds. Thus, the results obtained guide decision‐making in production systems, which are currently based on studies where the compounds were evaluated acting in isolation.

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Section: Resultscontrasting

confidence: 82%

mentioning

confidence: 53%

“…The concentrations chosen for the tests were based on the CL 50 96h determined by Dutra et al. (2016a, 2016b).…”

Section: Methodsmentioning

confidence: 99%