Adaptions of the mineral composition of tissue culture media on the basis of plant elemental analysis and composition of hydroponic substrates

Bouman, H.; Tiekstra, A.

doi:10.1007/1-4020-3200-5_37

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Therefore, it is not surprising that we obtained a better morphogenic response when the Cu concentration was raised. In our experiments, the optimum concentration was 20 µM, a concentration slightly higher than mentioned in many earlier reports (Dahleen 1995, Kintzios et al 2001, Nirwan and Kothari 2003, Nas and Read 2004, Bouman and Tiekstra 2005. All these authors reported a strong increase in growth when Cu is added at concentrations of 1.0 -5.0 µM.…”

Section: Discussionmentioning

confidence: 52%

Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina

Ahmad¹,

Alatar²,

Faisal³

et al. 2015

Biologia plant.

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The present study exemplifies morphogenic roles played by copper and zinc during micropropagation of Rauvolfia serpentina, an important medicinal shrub. Incorporation of 20 µM CuSO 4 or 25 µM ZnSO 4 to a Murashige and Skoog (MS) medium with optimized concentrations of auxins and cytokinins induced a maximum number of shoots per explant (40.67 ± 1.76 and 45.47 ± 0.24, respectively). However, higher concentrations of both the micronutrients negatively affected the morphogenic potential. The pigment content of the regenerants increased up to the optimal concentrations of both metals and thereafter decreased, whereas the maximum proline content was at the highest concentrations used. In vitro rooting of healthy shoots was accomplished using 0.5 µM IBA in a half strength liquid MS medium with 8.20 ± 0.37 roots, and root length of 5.50 ± 0.14 cm per microshoot. The plants survived a hardening procedure and were successfully acclimatized to field conditions with 95 % survival.

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Section: Discussionmentioning

confidence: 52%

Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina

Ahmad¹,

Alatar²,

Faisal³

et al. 2015

Biologia plant.

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“…Nas and Read (2004) proposed that the composition of a culture medium for a particular species should resemble the seed composition. Efficient multiplications were achieved by Bouman and Tiekstra (2005) using media with macronutrients resembling the elemental composition in adult leaves of Gerbera and Cymbidium. In the case of Weigela, a study found that manganese (Mn) and zinc (Zn) concentrations in leaves of W. florida were 28 and 27 mg • kg -1 , respectively (Chong et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Weigela florida ‘Tango’ through In Vitro Shoot Culture

Wang¹,

Li²,

Zeng³

et al. 2017

horts

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Weigela florida (Bunge) A. DC. is a popular flowering shrub adapted to a wide range of environmental conditions. Efficient methods for micropropagation of this species have not been well developed. The present study established a protocol for in vitro shoot culture of W. florida ‘Tango’ after a systematic evaluation of different culture media, cytokinins, and auxins on axillary shoot induction. Single-node stems were cultured on Driver and Kuniyuki Walnut (DKW) medium for initial production of axillary shoots. The shoots were used as explants and cultured on DKW medium supplemented with 8.88 μm 6-benzylaminopurine (BA) and 0.27 μm naphthaleneacetic acid (NAA), resulting in the production of more than six axillary shoots per explant. The axillary shoots could either be used as explants for additional shoot production or be cultured on ½ DKW medium supplemented with 0.25 μm indole-3-butyric acid (IBA) for rooting. Plantlets were transplanted into a substrate with 99% survival rate in a shaded greenhouse. This established method could be used for rapid propagation of W. florida to speed the introduction of new hybrids or cultivars for commercial production.

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“…Geary et al [ 11 ] established deficient, adequate, and excess N nutrient status in potato plants under hydroponic conditions by evaluating N content. No studies like this have been done for potato tissue culture, but the nutrient status has been successfully determined in tissue culture studies of other crops, including peach almonds, flowers, and legume trees [ 12 , 13 , 14 ]. The adoption of this approach may thus aid in growing potato plants with insufficient or excessive nutrient content in a tissue culture system.…”

Section: Introductionmentioning

confidence: 99%

A Model Nutrition Control System in Potato Tissue Culture and Its Influence on Plant Elemental Composition

Munthali

Kinoshita

Onishi

et al. 2022

Plants

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Low or excessive soil fertility is a major constraint to potato production. The influence of each individual nutrient element on potato plants under field studies remains ambiguous due to the influence of environmental variations. Creating an in vitro model plant with deficient or excessive nutrient content will provide a more controlled study and allow for a better understanding of how the concentration of one element can affect the uptake of other elements. Here we designed a tissue culture-based nutrition control system to systematically analyze the effects of essential nutrients on potato plants. Insufficient or excessive nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) contents were created by modifying the Murashige and Skoog (MS) medium. Deficient to toxic plant nutrient statuses were successfully defined by the evaluation of dry biomass and morphological symptoms. The results showed that plant shoot growth, nutrient uptake and content, and nutrient interactions were all significantly impacted by the changes in the MS media nutrient concentrations. These tissue culture systems can be successfully used for further investigations of nutrient effects on potato production in response to biotic and abiotic stresses in vitro.

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Adaptions of the mineral composition of tissue culture media on the basis of plant elemental analysis and composition of hydroponic substrates

Cited by 6 publications

References 14 publications

Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina

Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina

Micropropagation of Weigela florida ‘Tango’ through In Vitro Shoot Culture

A Model Nutrition Control System in Potato Tissue Culture and Its Influence on Plant Elemental Composition

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