Addition of nanoscaledbioinspiredsurface features: A revolution for bone related implants and scaffolds?

Bruinink, A.; Bitar, Malak; Pleskova, Miriam; Wick, Peter; Krug, Harald F.; Maniura‐Weber, Katharina

doi:10.1002/jbm.a.34691

Cited by 49 publications

(36 citation statements)

References 259 publications

(305 reference statements)

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“…3,4 Microscale manipulation is known to be able to alter cell migration and nutrient flow, whereas alterations in the nanoscale topography plays a key role in the induction of stem cell differentiation and osteogenesis. 5 Fused deposition modeling (FDM) has been developed as a promising method for macro-and micro-structuring in polycaprolactone (PCL) scaffold manufacturing. 6,7 Utilizing FDM, each scaffold can be custom-made to accommodate challenges of different bone void dimensions without losing the desirable feature of scalable and cost-effective industrial production.…”

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confidence: 99%

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Jensen

Kraft

Lysdahl

et al. 2015

Tissue Engineering Part A

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In this study, we sought to assess the osteogenic potential of human dental pulp stem cells (DPSCs) on three different polycaprolactone (PCL) scaffolds. The backbone structure of the scaffolds was manufactured by fused deposition modeling (PCL scaffold). The composition and morphology was functionalized in two of the scaffolds. The first underwent thermal induced phase separation of PCL infused into the pores of the PCL scaffold. This procedure resulted in a highly variable micro- and nanostructured porous (NSP), interconnected, and isotropic tubular morphology (NSP-PCL scaffold). The second scaffold type was functionalized by dip-coating the PCL scaffold with a mixture of hyaluronic acid and β-TCP (HT-PCL scaffold). The scaffolds were cylindrical and measured 5 mm in height and 10 mm in diameter. They were seeded with 1×10(6) human DPSCs, a cell type known to express bone-related markers, differentiate into osteoblasts-like cells, and to produce a mineralized bone-like extracellular matrix. DPSCs were phenotypically characterized by flow cytometry for CD90(+), CD73(+), CD105(+), and CD14(-). DNA, ALP, and Ca(2+) assays and real-time quantitative polymerase chain reaction for genes involved in osteogenic differentiation were analyzed on day 1, 7, 14, and 21. Cell viability and distribution were assessed on day 1, 7, 14, and 21 by fluorescent-, scanning electron-, and confocal microscopy. The results revealed that the DPSCs expressed relevant gene expression consistent with osteogenic differentiation. The NSP-PCL and HT-PCL scaffolds promoted osteogenic differentiation and Ca(2+) deposition after 21 days of cultivation. Different gene expressions associated with mature osteoblasts were upregulated in these two scaffold types, suggesting that the methods in which the scaffolds promote osteogenic differentiation, depends on functionalization approaches. However, only the HT-PCL scaffold was also able to support cell proliferation and cell migration resulting in even cell dispersion throughout the scaffold. In conclusion, DPSCs could be a possible alternate cell source for bone tissue engineering. The HT-PCL scaffold showed promising results in terms of promoting cell migration and osteogenic differentiation, which warrants future in vivo studies.

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confidence: 99%

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Jensen

Kraft

Lysdahl

et al. 2015

Tissue Engineering Part A

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“…We know that this interface plays a significant role in osseointegration of implants, but evidence based nanoscaled surfaced design is an elusive technique. 123 Further understanding of surface characteristics such as wettability, surface energy and roughness, surface curvature and nanoscale features, organic and inorganic coatings effect cell signaling, proliferation, integration, and viability will be required. In the foreseeable future, nanotechnology may allow for specifically tailored treatment of various disease states with complex skeletal defects at distinct anatomical sites.…”

Section: Resultsmentioning

confidence: 99%

Nanotechnology in bone tissue engineering

Walmsley

McArdle

Tevlin

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

239

162

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Nanotechnology represents a major frontier with potential to significantly advance the field of bone tissue engineering. Current limitations in regenerative strategies include impaired cellular proliferation and differentiation, insufficient mechanical strength of scaffolds, and inadequate production of extrinsic factors necessary for efficient osteogenesis. Here we review several major areas of research in nanotechnology with potential implications in bone regeneration: 1) nanoparticle-based methods for delivery of bioactive molecules, growth factors, and genetic material, 2) nanoparticle-mediated cell labeling and targeting, and 3) nano-based scaffold construction and modification to enhance physicochemical interactions, biocompatibility, mechanical stability, and cellular attachment/survival. As these technologies continue to evolve, ultimate translation to the clinical environment may allow for improved therapeutic outcomes in patients with large bone deficits and osteodegenerative diseases.

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“…The benefits are simpler and cleaner 'green' synthesis and increased biological interactivity and function [51][52][53][54].…”

Section: Biomineralization Understandingmentioning

confidence: 99%

Calcifying tissue regeneration via biomimetic materials chemistry

Green

Goto

Kim

et al. 2014

J. R. Soc. Interface.

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Materials chemistry is making a fundamental impact in regenerative sciences providing many platforms for tissue development. However, there is a surprising paucity of replacements that accurately mimic the structure and function of the structural fabric of tissues or promote faithful tissue reconstruction. Methodologies in biomimetic materials chemistry have shown promise in replicating morphologies, architectures and functional building blocks of acellular mineralized tissues dentine, enamel and bone or that can be used to fully regenerate them with integrated cell populations. Biomimetic materials chemistry encompasses the two processes of crystal formation and mineralization of crystals into inorganic formations on organic templates. This review will revisit the successes of biomimetics materials chemistry in regenerative medicine, including coccolithophore simulants able to promote in vivo bone formation. In-depth knowledge of biomineralization throughout evolution informs the biomimetic materials chemist of the most effective techniques for regenerative framework construction exemplified via exploitation of liquid crystals (LCs) and complex self-organizing media. Therefore, a new innovative direction would be to create chemical environments that perform reaction–diffusion exchanges as the basis for building complex biomimetic inorganic structures. This has evolved widely in biology, as have LCs, serving as self-organizing templates in pattern formation of structural biomaterials. For instance, a study is highlighted in which artificially fabricated chiral LCs, made from bacteriophages are transformed into a faithful copy of enamel. While chemical-based strategies are highly promising at creating new biomimetic structures there are limits to the degree of complexity that can be generated. Thus, there may be good reason to implement living or artificial cells in ‘morphosynthesis’ of complex inorganic constructs. In the future, cellular construction is probably key to instruct building of ultimate biomimetic hierarchies with a totality of functions.

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Addition of nanoscaledbioinspiredsurface features: A revolution for bone related implants and scaffolds?

Cited by 49 publications

References 259 publications

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Nanotechnology in bone tissue engineering

Calcifying tissue regeneration via biomimetic materials chemistry

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