Additively manufactured macroporous chambers facilitate large volume soft tissue regeneration from adipose-derived extracellular matrix

Guo, Zhang; Ci, Hai; Ma, Cambridge; Li, Zhipeng; Jiang, Wenbin; Chen, Lifeng; Wang, Zhenxing; Zhou, Muran; Sun, Jiaming

doi:10.1016/j.actbio.2022.05.053

Cited by 14 publications

(11 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The d-ECM serves as a biomaterial for filling in soft tissue defects. 149 Compared to the materials for long-term medical implants, the d-ECM is more conducive to promoting functional tissue regeneration. However, the quality of the decellularized tissue after implantation is decided not only by the transplantation site and size but also by its stability and regen-eration in the organism, which is strongly related to the immune response.…”

Section: Degradability Inflammation and Functional Tissue Remodeling ...mentioning

confidence: 99%

Decellularized extracellular matrix (d-ECM): the key role of the inflammatory process in pre-regeneration after implantation

et al. 2023

View full text Add to dashboard Cite

show abstract

Section: Degradability Inflammation and Functional Tissue Remodeling ...mentioning

confidence: 99%

Decellularized extracellular matrix (d-ECM): the key role of the inflammatory process in pre-regeneration after implantation

et al. 2023

View full text Add to dashboard Cite

show abstract

“…For example, a sustained basic fibroblast growth factor (bFGF) drug delivery system facilitated large volume soft fat tissue regeneration and reduced fibrous capsule formation. 35 Among the strategies for various tissue and organ regeneration, Tra for inhibiting fibrosis formation has been extensively investigated. 36 For example, a sustained Tra delivery system that is based on poly(lactic- co -glycolic acid) (PLGA) is capable of effectively preventing fibrosis.…”

Section: Discussionmentioning

confidence: 99%

Polydopamine-assisted tranilast immobilization on a PLA chamber to enhance fat flaps regeneration by reducing tissue fibrosis

et al. 2023

View full text Add to dashboard Cite

show abstract

“…By combining bioresorbable polycaprolactone and DAT, Zhang et al . fabricated porous bioscaffolds that successfully induced the formation of highly vascularized adipose tissue in a rabbit model [ 30 ]. However, such chemical approaches have negative effects on adipose regeneration due to excessive destruction of biologically loaded factors in DAT or residual chemical cross-linking agents.…”

Section: Discussionmentioning

confidence: 99%

Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration

et al. 2023

Burns &Amp; Trauma

View full text Add to dashboard Cite

Background Large-area soft tissue defects are challenging to reconstruct. Clinical treatment methods are hampered by problems associated with injury to the donor site and the requirement for multiple surgical procedures. Although the advent of decellularized adipose tissue (DAT) offers a new solution to these problems, optimal tissue regeneration efficiency cannot be achieved because the stiffness of DAT cannot be altered in vivo by adjusting its concentration. This study aimed to improve the efficiency of adipose regeneration by physically altering the stiffness of DAT to better repair large-volume soft tissue defects. Methods In this study, we formed three different cell-free hydrogel systems by physically cross-linking DAT with different concentrations of methyl cellulose (MC; 0.05, 0.075 and 0.10 g/ml). The stiffness of the cell-free hydrogel system could be regulated by altering the concentration of MC, and all three cell-free hydrogel systems were injectable and moldable. Subsequently, the cell-free hydrogel systems were grafted on the backs of nude mice. Histological, immunofluorescence and gene expression analyses of adipogenesis of the grafts were performed on days 3, 7, 10, 14, 21 and 30. Results The migration of adipose-derived stem cells (ASCs) and vascularization were higher in the 0.10 g/ml group than in the 0.05 and 0.075 g/ml groups on days 7, 14 and 30. Notably, on days 7, 14 and 30, the adipogenesis of ASCs and adipose regeneration were significantly higher in the 0.075 g/ml group than in the 0.05 g/ml group (p < 0.01 or p < 0.001) and 0.10 g/ml group (p < 0.05 or p < 0.001). Conclusion Adjusting the stiffness of DAT via physical cross-linking with MC can effectively promote adipose regeneration, which is of great significance to the development of methods for the effective repair and reconstruction of large-volume soft tissue defects.

show abstract

Additively manufactured macroporous chambers facilitate large volume soft tissue regeneration from adipose-derived extracellular matrix

Cited by 14 publications

References 57 publications

Decellularized extracellular matrix (d-ECM): the key role of the inflammatory process in pre-regeneration after implantation

Decellularized extracellular matrix (d-ECM): the key role of the inflammatory process in pre-regeneration after implantation

Polydopamine-assisted tranilast immobilization on a PLA chamber to enhance fat flaps regeneration by reducing tissue fibrosis

Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration

Contact Info

Product

Resources

About