Adenosine Deaminase Isoenzymes in the Diagnosis and Monitoring of Rheumatoid Arthritis

Abstract

The aim of this study was determination of the cata ly tic activities of adenosine deaminase (ADA), ADA1 and ADA2 isoenzymes in the serum of patients suffering from rheumatoid arthritis (RA) who were and were not treated with methotrexate (MTX), and identification of the possibilities of using these biochemical parameters in diagnosing and monitoring the treatment effects in RA. Catalytic activities of total ADA (tADA) and ADA2 in serum were determined by a spectrophotometric method. A statistically significant correlation was found between the total ADA and ADA1 values, as well as between tADA and ADA2 in the serum of all patients suffering from RA. Determination of ADA1 and ADA2 isoenzyme catalytic activities in the serum of patients who might be suffering from RA improves the diagnostic value of total ADA catalytic activity determination. ADA2 catalytic activity in serum can be a useful biochemical marker in diagnosing and monitoring RA. Decrease in ADA1 isoenzyme catalytic activities in the serum of patients suffering from RA who were treated with MTX can help in the observation of MTX therapy effects.Kratak sadr`aj: Cilj rada bilo je odre|ivanje kataliti~kih aktiv nosti izoenzima adenozin deaminaze (ADA), ADA1 i ADA2 u serumima obolelih od reumatoidnog artritisa (RA) le~enih uz pomo} ili bez terapije metotreksatom (MTX), kao i utvr |i vanje mogu}nosti uvo|enja ovih biohemijskih parametara u dijagnostiku i pra}enje terapijskih efekata u RA. Kataliti~ke aktivnosti ukupne ADA (tADA) i ADA2 u serumima odre|i vane su spektrofotometrijskom metodom. Utvr|e na je stati sti ~ki zna~ajna korelacija izme|u kataliti~kih aktivnosti tADA i ADA1, kao i tADA i ADA2 u serumima svih ispitanika sa RA. Odre|ivanje kataliti~kih aktivnosti izoenzima ADA1 i ADA2 u serumima pacijenata sa sumnjom na RA pobolj{ava dijagnosti~ku vrednost odre|ivanja kataliti~ke aktivnosti tADA. Kata li ti ~ka aktivnost ADA2 u serumu mo`e da bude koristan bio he mijski marker u dijagnostici i pra}enju RA. Sma njenje ka ta li ti~kih aktivnosti izoenzima ADA1 u serumima pacijenata sa RA koji su dobijali MTX mo`e da pomogne u pra}enju terapijskih efekata MTX.Klju~ne re~i: izoenzimi adenozin deaminaze, metotreksat, reumatoidni artritis

Increased Chitotriosidase Activities in Patients with Rheumatoid Arthritis: A Possible Novel Marker?

“…In this regard, serum YKL-40 levels increase even in early RA patients (22,33). In a recent study, it has been proposed that a »multi-biomarker disease activity score« which was calculated using the concentrations of twelve biomarkers, including YKL-40, measured disease activity (34,35). However, as seen in the present study, the low sensitivity of serum YKL-40 for RA might be an obstacle to its clinical use.…”

Serum Adenosine Deaminase as Inflammatory Marker in Rheumatoid Arthritis

“…Studies conducted to evaluate the role of ADA in patients with rheumatoid arthritis showed varied results. Nalesnik M et al, reported significant correlation between ADA and CRP in patients with RA without methotrexate treatment and suggested that ADA activity in serum can be used as a biochemical marker of the inflammatory process in RA [16]. In an earlier study, Yuksel and colleague [17] measured serum and synovial fluid ADA activity in patients with Rheumatoid arthritis, osteoarthritis and reactive arthritis.…”

“…The enzyme ADA represents a checkpoint in the regulation of extra cellular Adenosine levels [8] and thus is likely to modulate the inflammatory processes. Hence, although determination of serum adenosine levels is an appropriate way to assess disease activity in RA [8], ADA also seems to be a prediction marker of the inflammatory process in RA [16]. In this context, Zamani et al, [21] studied the relationship between serum adenosine deaminase and disease activity and concluded that a new disease activity index applying serum ADA helps in predicting disease activity in RA.…”

Serum Adenosine Deaminase and IL 6 as Inflammatory Marker in Rheumatoid Arthritis

“…The autoantibodies and high level of inflammatory markers help in diagnosis while they are also important for prognosis and diagnosis of treatment. 3 The biological markers such as acute phase reactants, rheumatoid factor (RF) etc. acts as an indicator of the disease progression besides the number of swollen and tender joints.…”