Adenosine-Triphosphate in Trauma-Related and Elective Hypothermia

Seekamp, Andreas; Griensven, Martijn van; Hildebrandt, F.; Wahlers, T; Tscherne, H.

doi:10.1097/00005373-199910000-00011

Cited by 67 publications

(29 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite benefits of hypothermia in elective surgery, in certain trauma cases hypothermia is one of the most frequent causes of posttraumatic complications. Hypothermia induced by active cooling in elective surgery preserves well the ATP pool maintaining the aerobic metabolism, while in trauma patients hypothermia caused by decreased heat generation leads to ATP depletion under anaerobic metabolic condition 15 . Cellular depletion of ATP is in connection with the irreversibility of ischemic processes 29 .…”

Section: Effects Of Coolingmentioning

confidence: 99%

“…Further questions rise in respect of cooling in traffic or crush accidents, or following traumatic limb amputation. Despite of the beneficial effect of preventive cooling, its optimum has not been clarified yet, furthermore, controversial data suggests that cooling does not mean always an advantage [12][13][14][15][16] . We hypothesized that limb ischemia-reperfusion at normo-or hypothermia variously alters blood coagulation parameters.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hematological and hemostaseological alterations after warm and cold limb ischemia-reperfusion in a canine model

et al. 2009

View full text Add to dashboard Cite

PURPOSE: Acute ischemia-reperfusion (I/R) of extremities means serious challenge in the clinical practice. Furthermore, the issue of preventive cooling is still controversial. In this canine model we investigated whether limb I/R -with or without cooling- has an influence on hematological and hemostaseological factors. METHODS: Femoral vessels were exposed and clamped for 3 hours. After release the clamps, 4-hour reperfusion was secured. The same procedures with cooling using ice bags, as well as warm and cold sham-operations were performed. Before operations, from the excluded limb by the end of ischemia, during the reperfusion, and for 5 postoperative days afterwards blood samples were collected for testing hematological and blood coagulation parameters. RESULTS: After I/R activated partial thromboplastin time was elongated on 2nd-4th postoperative days. The highest values were on the 2nd day in cold I/R group, accompanied by increased prothrombin time values. The hematological parameters and fibrinogen level showed non-specific changes. In excluded ischemic limb the blood composition showed controversial data. Cold ischemia induced larger alterations, however platelet count, hematocrit changed more expressly in warm ischemia. CONCLUSION: These results indicate the risk of coagulopathy following limb I/R on early post-eventually days, which risk is higher in the case of cold I/R.

show abstract

Section: Effects Of Coolingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hematological and hemostaseological alterations after warm and cold limb ischemia-reperfusion in a canine model

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The important mechanisms associated with these beneficial effects are maintaining aerobic metabolism and reducing oxygen demand (20,21). Similar mechanisms have also enabled induced hypothermia to improve oral and gastric mucosal microvascular oxygenation under hypoxic conditions (22).…”

Section: Introductionmentioning

confidence: 99%

Induced Hypothermia Preserves the Functional Enterocyte Mass in A Porcine Multiple Trauma Model

Fröhlich¹,

Maegele²,

Hildebrand³

et al. 2016

SHOCK

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plasma ATP levels fall with shock (Jabs et al, 1979;Seekamp et al, 1999), but these measurements may be confounded by failure to account for ATP release from blood elements and extracellular ATP catabolism (Gorman et al, 2007). Newer analytical methods show that ATP levels in some pericellular environments may be in the range of several hundred micromolar (Abbracchio et al, 2006;Bours et al, 2006;Pellegatti et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Dexamethasone Enhances ATP-Induced Inflammatory Responses in Endothelial Cells

Ding¹,

Gao²,

Suffredini³

2010

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The purinergic nucleotide ATP is released from stressed cells and is implicated in vascular inflammation. Glucocorticoids are essential to stress responses and are used therapeutically, yet little information is available that describes the effects of glucocorticoids on ATP-induced inflammation. In a human microvascular endothelial cell line, extracellular ATP-induced interleukin (IL)-6 secretion in a dose-and time-dependent manner. When cells were pretreated with dexamethasone, a prototypic glucocorticoid, ATP-induced IL-6 production was enhanced in a time-and dose-dependent manner. Mifepristone, a glucocorticoid receptor antagonist, blocked these effects. ATP-induced IL-6 release was significantly inhibited by a phospholipase C inhibitor (88 Ϯ 1%, p Ͻ 0.001). Cells treated with dexamethasone induced mRNA expression of the purinergic P2Y 2 receptor (P2Y 2 R) 1.8-Ϯ 0.1-fold and, when stimulated with ATP, enhanced Ca 2ϩ release and augmented IL-6 mRNA expression. Silencing of the P2Y 2 R by its small interfering RNA decreased ATP-induced IL-6 production by 81 Ϯ 1% (p Ͻ 0.001). Dexamethasone enhanced the transcription rate of P2Y 2 R mRNA and induced a dose-related increase in the activity of the P2Y 2 R promoter. Furthermore, dexamethasoneenhanced ATP induction of adhesion molecule transcription and augmented the release of IL-8. Dexamethasone leads to an unanticipated enhancement of endothelial inflammatory mediator production by extracellular ATP via a P2Y 2 R-dependent mechanism. These data define a novel positive feedback loop of glucocorticoids and ATP-induced endothelial inflammation.

show abstract

Adenosine-Triphosphate in Trauma-Related and Elective Hypothermia

Cited by 67 publications

References 29 publications

Hematological and hemostaseological alterations after warm and cold limb ischemia-reperfusion in a canine model

Hematological and hemostaseological alterations after warm and cold limb ischemia-reperfusion in a canine model

Induced Hypothermia Preserves the Functional Enterocyte Mass in A Porcine Multiple Trauma Model

Dexamethasone Enhances ATP-Induced Inflammatory Responses in Endothelial Cells

Contact Info

Product

Resources

About