Adherence of Gallibacterium anatis to Inert Surfaces

Vaca, Sergio; Monroy, Eric; Rojas, Lourdes; Vázquez, Candelario; Sánchez, Patricio; Soriano-Va, E.; Bojesen, Anders Miki; Negrete-Abascal, Erasmo

doi:10.3923/javaa.2011.1688.1693

Cited by 17 publications

(21 citation statements)

References 17 publications

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“…Strains F149 T , CCM5974 and 12158/5 salp. have previously been shown to be strong at biofilm formation, which to some degree validates the current results [41]. Many isolates from the Iowa collection, though, were weak biofilm formers that belonged outside of the aforementioned clusters.…”

Section: Resultssupporting

confidence: 89%

Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

et al. 2013

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Peritonitis is the major disease problem of laying hens in commercial table egg and parent stock operations. Despite its importance, the etiology and pathogenesis of this disease have not been completely clarified. Although avian pathogenic Escherichia coli (APEC) isolates have been incriminated as the causative agent of laying hen peritonitis, Gallibacterium anatis are frequently isolated from peritonitis lesions. Despite recent studies suggesting a role for G. anatis in the pathogenesis of peritonitis, little is known about the organism’s virulence mechanisms, genomic composition and population dynamics. Here, we compared the genome sequences of three G. anatis isolates in an effort to understand its virulence mechanisms and identify novel antigenic traits. A multilocus sequence typing method was also established for G. anatis and used to characterize the genotypic relatedness of 71 isolates from commercial laying hens in Iowa and 18 international reference isolates. Genomic comparisons suggest that G. anatis is a highly diverse bacterial species, with some strains possessing previously described and potential virulence factors, but with a core genome containing several antigenic candidates. Multilocus sequence typing effectively distinguished 82 sequence types and several clonal complexes of G. anatis, and some clones seemed to predominate among G. anatis populations from commercial layers in Iowa. Biofilm formation and resistance to antimicrobial agents was also observed in several clades. Overall, the genomic diversity of G. anatis suggests that multiple lineages exist with differing pathogenic potential towards birds.

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Section: Resultssupporting

confidence: 89%

Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

et al. 2013

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“…However, applying transmission electron microscopy, the fimbrial structure has been suggested for initial bacterial colonization of G. anatis in oropharyngeal epithelial cells (Lucio et al, 2012). Furthermore, these bacteria have been found to create robust biofilms at inert surfaces (Vaca et al, 2011). Finally, it remains to be elucidated whether G. anatis displays a defence mechanism against phagocytation by macrophages as described for Escherichia coli (Pourbakhsh et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Assessing pathogenicity ofGallibacterium anatisin a natural infection model: the respiratory and reproductive tracts of chickens are targets for bacterial colonization

et al. 2013

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“…anatis is a major cause of salpingitis and peritonitis in egglaying chickens, leading to decreased egg production and increased mortality worldwide. It has previously been suggested that different means of attachment and the ability to form biofilms are important for G. anatis to overcome the propulsive effect of peristalsis and allow colonization of the upper reproductive tract (19), and yet specific factors and their contributions to these processes have remained unknown. Here we have characterized an F17-like fimbrial subunit protein from G. anatis and demonstrated that it is expressed and surface exposed in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Preventing adherence is therefore an attractive intervention strategy against further negative effects (18). Although G. anatis can adhere to plastic surfaces, form biofilms, and produce a glycoprotein-like hemagglutinin (19,20), little is known about the importance of these factors during colonization and infection of the natural host. A variety of adhesins, including fimbriae, has been reported from other members of Pasteurellaceae (21).…”

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confidence: 99%

The Fimbrial Protein FlfA from Gallibacterium anatis Is a Virulence Factor and Vaccine Candidate

et al. 2013

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cThe Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in egg-laying chickens, leading to decreased egg production worldwide. Widespread multidrug resistance largely prevents treatment of this organism using traditional antimicrobial agents, while antigenic diversity hampers disease prevention by classical vaccines. Thus, insight into its pathogenesis and knowledge about important virulence factors is urgently required. A key event during the colonization and invasion of mucosal surfaces is adherence, and recently, at least three F17-like fimbrial gene clusters were identified in the genomes of several G. anatis strains. The objective of this study was to characterize the putative F17-like fimbrial subunit protein FlfA from G. anatis 12656-12 and determine its importance for virulence. In vitro expression and surface exposure of FlfA was demonstrated by flow cytometry and immunofluorescence microscopy. The predicted function of FlfA as a fimbrial subunit protein was confirmed by immunogold electron microscopy. An flfA deletion mutant (⌬flfA) was generated in G. anatis 12656-12, and importantly, this mutant was significantly attenuated in the natural chicken host. Furthermore, protection against G. anatis 12656-12 could be induced by immunizing chickens with recombinant FlfA. Finally, in vitro expression of FlfA homologs was observed in a genetically diverse set of G. anatis strains, suggesting the potential of FlfA as a serotype-independent vaccine candidate This is the first study describing a fimbrial subunit protein of G. anatis with a clear potential as a vaccine antigen.

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Adherence of Gallibacterium anatis to Inert Surfaces

Cited by 17 publications

References 17 publications

Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

Assessing pathogenicity ofGallibacterium anatisin a natural infection model: the respiratory and reproductive tracts of chickens are targets for bacterial colonization

The Fimbrial Protein FlfA from Gallibacterium anatis Is a Virulence Factor and Vaccine Candidate

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