Adhesion of fibroblast cells on thin films representing surfaces of polymeric scaffolds of human urethra rationalized by molecular models of integrin binding

Braccini, Simona; Pecorini, Gianni; Chiellini, Emo; Bakoš, Dušan; Miertus, Stanislav; Frecer, Vladimı́r

doi:10.1016/j.jbiotec.2020.11.001

Cited by 8 publications

(8 citation statements)

References 27 publications

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“…While biocompatibility evaluation comprises several biological parameters, cytocompatibility is one of the easiest to assess, as it can provide information about the cellular toxicity and adhesion, which are both important for tissue regeneration [ 63 ]. Embryonic fibroblast cells have been much described in the literature for studies on the cytocompatibility and cell adhesion in materials [ 65 ]; thus, they were chosen to evaluate the formulation performances in vitro. Further, fibroblasts are a differentiated cellular type of keratocytes in the stroma, and they participate in the immune system, differentiation, and corneal wound healing [ 66 ].…”

Section: Resultsmentioning

confidence: 99%

Mimicking the Physicochemical Properties of the Cornea: A Low-Cost Approximation Using Highly Available Biopolymers

Hernández,

Panadero-Medianero,

Arrázola

et al. 2024

Polymers

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Corneal diseases represent a significant global health challenge, often resulting in blindness, for which penetrating keratoplasty is the clinical gold standard. However, in cases involving compromised ocular surfaces or graft failure, osteo-odonto keratoprosthesis (OOKP) emerges as a vital yet costly and complex alternative. Thus, there is an urgent need to introduce soft biomaterials that mimic the corneal tissue, considering its translation’s physicochemical, biological, and economic costs. This study introduces a cross-linked mixture of economically viable biomaterials, including gelatin, chitosan, and poly-D-lysine, that mimic corneal properties. The physicochemical evaluation of certain mixtures, specifically gelatin, chitosan, and poly-D-lysine cross-linked with 0.10% glutaraldehyde, demonstrates that properties such as swelling, optical transmittance, and thermal degradation are comparable to those of native corneas. Additionally, constructs fabricated with poly-D-lysine exhibit good cytocompatibility with fibroblasts at 72 h. These findings suggest that low-cost biopolymers, particularly those incorporating poly-D-lysine, mimic specific corneal characteristics and have the potential to foster fibroblast survival. While further studies are required to reach a final corneal-mimicking solution, this study contributes to positioning low-cost reagents as possible alternatives to develop biomaterials with physicochemical properties like those of the human cornea.

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Section: Resultsmentioning

confidence: 99%

Mimicking the Physicochemical Properties of the Cornea: A Low-Cost Approximation Using Highly Available Biopolymers

Hernández,

Panadero-Medianero,

Arrázola

et al. 2024

Polymers

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“…Over the past few decades, tremendous efforts have resulted in fabricating various artificial tissue constructs using biomaterials based on organic and inorganic-based materials for tissue regeneration ( Braccini et al, 2020 ). In addition to tissue growth, these biomaterials can be applied for vascularization and nerve innervation, improving the tissue repair efficiency of these implanted scaffolds ( Langer and Vacanti, 1993 ).…”

Section: Significance Of Microarchitecturesmentioning

confidence: 99%

“…The regulated interplay and the intricately controlled crosstalk between the materials and the biological components played substantial roles in TE and RM toward tissue growth ( Khademhosseini and Langer, 2016 ). However, various key attributes, such as growth factors and their precise actions, would play the predominant role in cell proliferation, requiring them in the engineered constructs in ex-vivo and in vivo ( Kankala et al, 2018a ; Braccini et al, 2020 ). Despite the success, the large-sized scaffolds require sophisticated, highly invasive surgical procedures, leaving a scar ( Wei et al, 2018 ).…”

Section: Significance Of Microarchitecturesmentioning

confidence: 99%

“…To a considerable extent, the selection of polymer mainly plays a vital role in fabricating microcarriers for cell delivery. Based on the source of origination, different kinds of polymers have been employed to fabricate these 3D microcarriers (non-porous and porous) for cell delivery applications, such as natural (gelatin, dextran, cellulose, chitin and its derivatives, and alginic acid) ( Bidarra et al, 2014 ; Ho et al, 2020 ; Lavanya et al, 2020 ; Rahman et al, 2021 ; Liang et al, 2022 ; Liao et al, 2022 ), and synthetic (PLGA, PLA, silk fibroin, SF, polyurethane, polyacrylamide, and PHEMA) ( Hong et al, 2005 ; Hafeman et al, 2008 ; Braccini et al, 2020 ). Considering the pros and cons, polymers from natural sources possess high biocompatibility due to biomolecules, recyclability, and mechanical properties ( Rahman et al, 2021 ).…”

Section: Factors Influencing Cell Deliverymentioning

confidence: 99%

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Polymeric microcarriers for minimally-invasive cell delivery

Duan

Yu²,

Hu³

et al. 2023

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims at restoring tissue defects by applying the three-dimensional (3D) biomimetic pre-formed scaffolds to restore, maintain, and enhance tissue growth. Broadly speaking, this approach has created a potential impact in anticipating organ-building, which could reduce the need for organ replacement therapy. However, the implantation of such cell-laden biomimetic constructs based on substantial open surgeries often results in severe inflammatory reactions at the incision site, leading to the generation of a harsh adverse environment where cell survival is low. To overcome such limitations, micro-sized injectable modularized units based on various biofabrication approaches as ideal delivery vehicles for cells and various growth factors have garnered compelling interest owing to their minimally-invasive nature, ease of packing cells, and improved cell retention efficacy. Several advancements have been made in fabricating various 3D biomimetic microscale carriers for cell delivery applications. In this review, we explicitly discuss the progress of the microscale cell carriers that potentially pushed the borders of TE, highlighting their design, ability to deliver cells and substantial tissue growth in situ and in vivo from different viewpoints of materials chemistry and biology. Finally, we summarize the perspectives highlighting current challenges and expanding opportunities of these innovative carriers.

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“…The protein of cell membranes can adhere to the hydrophobic surface, demonstrated by a water contact angle of h > 65f. For example, PEG has been commonly used as anti-adhesion layers due to good hydrophilicity, and thus the adjustment of the surface's hydrophilicity is an effective measure to improve the cell adhesion [47][48][49].…”

Section: Water Contact Angle Of Pva/cnt Electrospun Filmsmentioning

confidence: 99%

Biodegradable and conductive PVA/CNT nanofibrous membranes used in nerve conduit applications

Jhang

Lin

Lou

et al. 2021

Journal of Industrial Textiles

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The recovery of impaired peripheral nerves is often not as expected, which makes the development of nerve conduits trendy nowadays. To enable the neural messages effectively being delivered as well as to prevent the secondary damage during the removal of nerve conduits, the conductivity and biodegradability are two essential requirements for ideal nerve conduits. In this study, electrospinning is used to produce polyvinyl alcohol (PVA)/carbon nanotubes (CNT) electrospun films, after which the morphology analysis, electrical property, water contact angle, and biological characteristics of the membranes are investigated, thereby determining the optimal nerve conduits based on the employment of electrospinning, PVA, and CNT. The test results indicate that with 0.25 wt% of PVA, the electrospun films exhibit comparatively lower resistance of 25.3 ohm, good fibrous morphology with a diameter being 1 μm. In addition, the electrospun films are cytotoxicity-free and facilitate the growth of cells. It is observed in the MMT assay that after co-cultured with cells for three days, PVA/CNT electrospinning fibrous membranes exhibit a cellular viability that is 18.5 times greater than that of the control group on Day 1. According to all property evaluations, PVA/CNT electrospinning fibrous membranes are a qualified candidate for the use of nervous conduits.

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Adhesion of fibroblast cells on thin films representing surfaces of polymeric scaffolds of human urethra rationalized by molecular models of integrin binding

Cited by 8 publications

References 27 publications

Mimicking the Physicochemical Properties of the Cornea: A Low-Cost Approximation Using Highly Available Biopolymers

Mimicking the Physicochemical Properties of the Cornea: A Low-Cost Approximation Using Highly Available Biopolymers

Polymeric microcarriers for minimally-invasive cell delivery

Biodegradable and conductive PVA/CNT nanofibrous membranes used in nerve conduit applications

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