Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation

Castilho-Fernandes, Andrielle; Lopes, Tácila Gabriele; Ferreira, Fernanda Silva; Rezende, Nayara; Silva, Valéria F.; Primo, Fernando Lucas; Fontes, Aparecida Maria; Ribeiro‐Silva, Alfredo; Tedesco, Antônio Cláudio

doi:10.1016/j.pdpdt.2018.11.013

Cited by 15 publications

(6 citation statements)

References 42 publications

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“…The longest irradiation time (265 s), corresponding to an energy density of 3 J/cm 2 , resulted in a lower cell growth at the seventh day, with a considerable decrease in cell viability, suggesting that these parameters could have affected somehow the cell cycle in this period. These results differed from others, in which cells irradiated with low level laser presented a higher proliferation rate than non-irradiated cells 3,26,27 . Meanwhile, other studies demonstrated that irradiation with low-level laser did not interfere significantly in cell viability and proliferation as compared with the non-irradiated controls.…”

Section: Resultscontrasting

confidence: 99%

In Vitro Effect of Low-Level Laser Therapy on Undifferentiated Mouse Pulp Cells

Chimello-Sousa¹,

Bombonato-Prado²,

Fernandes³

et al. 2021

J. Health Scie.

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Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. The aim of this study was to analyze the influence of low-level laser therapy (λ=660 nm), with three different irradiation times, on the behavior of OD-21 cell line. The cells were cultivated and divided into three groups: non-irradiated/control (group I); irradiated with 88 s (group II); irradiated with 177 s (group III) and irradiated with 265 s (group IV). Cell growth and viability were assessed after 7 and 10 days. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α=.05). At day 7, there was a higher cell growth in groups I and II, as compared to group IV (p<.01). At the 10th day, group I showed a higher cell growth as compared to group II (p<.05). Cell viability in group IV was significantly lower at the 7th day, as compared to groups I (p<.001), II (p<.01) and III (p<.001). Cell viability in all the groups was over 80%, except in group IV at day 7. Irradiation time of group I influenced positively the proliferation and viability of OD-21 cells in late cell culture period. Keywords: Low-Level Laser Therapy. Cell Culture. Stem Cells. ResumoA terapia a laser de baixa intensidade tem sido investigada como possível estímulo para aumento da proliferação e diferenciação de vários tipos de células, mas poucos relatos relacionam a resposta de células indiferenciadas da polpa dentária de camundongos (OD-21) à irradiação em modelos in vitro. O objetivo deste estudo foi analisar a influência do laser de baixa intensidade (λ=660 nm), com três períodos de irradiação diferentes, no comportamento das células da linhagem OD-21. As células foram cultivadas e distribuídas em três grupos: não irradiado / controle (grupo I); irradiado com 88 s (grupo II); irradiado com 177 s (grupo III) e irradiado com 265 s (grupo IV). O crescimento e a viabilidade celular foram avaliados após 7 e 10 dias. Os dados foram analisados pelos testes de Kruskal-Wallis e Mann-Whitney (α = 0,05). No dia 7, houve crescimento celular maior nos grupos I e II, em comparação ao grupo IV (p <0,01). No décimo dia, o grupo I apresentou crescimento celular superior ao grupo II (p <0,05). A viabilidade celular no grupo IV foi significativamente menor no sétimo dia, em comparação aos grupos I (p <0,001), II (p <0,01) e III (p <0,001). A viabilidade celular em todos os grupos foi superior a 80%, exceto no grupo IV no dia 7. O tempo de irradiação do grupo I influenciou positivamente a proliferação e a viabilidade das células OD-21 no período mais tardio da cultura celular. Palavras-chave: Laserterapia de Baixa Intensidade. Cultura Celular. Células Tronco.

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Section: Resultscontrasting

confidence: 99%

In Vitro Effect of Low-Level Laser Therapy on Undifferentiated Mouse Pulp Cells

Chimello-Sousa¹,

Bombonato-Prado²,

Fernandes³

et al. 2021

J. Health Scie.

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“…The expression of lipoprotein lipase and PPARY mRNA also increased in this group. The structures of lipid vacuoles were also observed 37 . When the MSCs are used to treat damaged bones, it is essential to differentiate the MSCs into the bone cells, and this has already been studied, which has acknowledged the importance of this matter.…”

Section: Bone Marrow Mesenchymal Stem Cellsmentioning

confidence: 98%

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

et al. 2019

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Introduction: The purpose of this study is to investigate the effect of a low-power laser on the proliferation, migration, differentiation of different types of mesenchymal stem cells (MSCs) in different studies. Methods: The relevant articles that were published from 2004 to 2019 were collected from the sources of PubMed, Scopus, and only the articles specifically examining the effect of a lowpower laser on the proliferation, differentiation, and migration of the MSCs were investigated. Results: After reviewing the literature, only 42 articles were found relevant. Generally, most of the studies demonstrated that different laser parameters increased the proliferation, migration, and differentiation of the MSCs, except the results of two studies which were contradictory. In fact, changing the parameters of a low-power laser would affect the results. On the other hand, the source of the stem cells was reported as a key factor. In addition, the combination of lasers with other therapeutic approaches was found to be more effective. Conclusion: The different parameters of lasers has been found to be effective in the proliferation, differentiation, and migration of the MSCs and in general, a low-power laser has a positive effect on the MSCs, helping to improve different disease models.

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“…In other words, we verified that the new strategy using OLEDs is a way to overcome the problems of previous PBM while maintaining safety. Most studies conducted using irradiating LEDs or lasers on stem cells are controversial; they either revealed that the differentiation of stem cells is induced, 13 , 14 , 76 – 78 or that there is no change in differentiation 79 , 80 after light irradiation. However, the PBM of OLED irradiation did not induce differentiation in a specific direction and, instead, the stemness of hADSCs themselves increased.…”

Section: Discussionmentioning

confidence: 99%

“… 65 , 67 – 69 , 79 , 81 – 83 Moreover, when OLED light is applied to wound treatment, angiogenesis and wound healing can be promoted by stimulated secretion of various cytokines and growth factors as much as the efficiency of stem cells improves. 14 , 35 , 76 …”

Section: Discussionmentioning

confidence: 99%

Lightwave-reinforced stem cells with enhanced wound healing efficacy

Jeon

Kim

et al. 2021

J Tissue Eng

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Comprehensive research has led to significant preclinical outcomes in modified human adipose-derived mesenchymal stem cells (hADSCs). Photobiomodulation (PBM), a technique to enhance the cellular capacity of stem cells, has attracted considerable attention owing to its effectiveness and safety. Here, we suggest a red organic light-emitting diode (OLED)-based PBM strategy to augment the therapeutic efficacy of hADSCs. In vitro assessments revealed that hADSCs basked in red OLED light exhibited enhanced angiogenesis, cell adhesion, and migration compared to naïve hADSCs. We demonstrated that the enhancement of cellular capacity was due to an increased level of intracellular reactive oxygen species. Furthermore, accelerated healing and regulated inflammatory response was observed in mice transplanted with red light-basked hADSCs. Overall, our findings suggest that OLED-based PBM may be an easily accessible and attractive approach for tissue regeneration that can be applied to various clinical stem cell therapies.

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Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation

Cited by 15 publications

References 42 publications

In Vitro Effect of Low-Level Laser Therapy on Undifferentiated Mouse Pulp Cells

In Vitro Effect of Low-Level Laser Therapy on Undifferentiated Mouse Pulp Cells

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

Lightwave-reinforced stem cells with enhanced wound healing efficacy

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