Adipose stem cells controlled by surface chemistry

Abstract

This study investigated how human adipose stem cells (hASCs) could be influenced by surface chemistry. Self-assembled monolayers of alkanethiolates on gold were introduced as a surface chemistry model to provide a range of functional groups such as OH, COOH, NH₂, Phenyl, SH, Br, and CH₃ on surfaces. Initially, morphological changes of hASCs in response to different surface chemistries were observed with focal adhesion. Cell growth behaviour evaluated by Cell Counting Kit-8 (CCK8) assay (Dojindo Molecular Technologies Inc., Shanghai, China) and cytoskeletal F-actin Biochem Kit™ (Denver, CO, USA) staining revealed a descending order of growth rate on the following surfaces: NH₂ > SH > COOH > Phenyl > Br > OH > CH₃. The mRNA expressions of lineage specific markers including alkaline phosphatase (ALP), osteocalcin (OCN), type II collagen, aggrecan, peroxisome proliferator-activated receptor gamma (PPARγ), and fatty acid binding protein-2 (aP2), were determined using real-time reversed transcriptase-polymerase chain reaction (RT-PCR). Results revealed that NH₂ favoured hASC differentiation toward osteogenic, while phenyl and SH promoted chondrogenic differentiation of hASCs with a high level up-regulation of type II collagen and aggrecan. hASCs on Br increased in PPARγ and aP2 expression, indicating adipogenic differentiation. These results highlight the vital role of surface chemistry on the modulation of hASC differentiation and suggests chemical methods for designing biomaterials for stem cell-based tissue regeneration.

Activation of the ERK1/2 Signaling Pathway during the Osteogenic Differentiation of Mesenchymal Stem Cells Cultured on Substrates Modified with Various Chemical Groups

“…The water contact angle measurements of the four alkanethiol-terminated SAMs with functional groups (HS–(CH 2 ) 11 X, X = –COOH, –NH 2 , –OH, and –CH 3 ) were utilized, and the values of water contact angles were determined as previously described: –OH <–COOH <–NH 2 <–CH 3 [29–32]. …”

“…Following overnight assembly, the substrates were thoroughly rinsed with ethanol and distilled water. Finally, these substrates were placed in 24-well tissue culture polystyrene plates (Corning, USA), sterilized with 75% alcohol overnight, and then rinsed extensively with sterilized PBS [29]. …”

Human adipose CD34+CD90+ stem cells and collagen scaffold constructs grafted in vivo fabricate loose connective and adipose tissues

“…To successfully create a 3D structure capable of supporting tissue formation, several key roles of tissue engineering need to be considered, including pores with interconnected architecture; material degradability; biocompatibility with the seeded cells to enhance cellular activity [Kuberka et al, 2002; O'Brien et al, 2005]. Therefore, the formation of a new tissue in the 3D matrix structure is highly influenced by the chemical composition of the scaffold [Cassell et al, 2001; Liu et al, 2011]. The extracellular matrix (ECM) is recognized as a highly hydrated network that comprises several main effectors.…”

Directing the fate of human and mouse mesenchymal stem cells by hydroxyl–methyl mixed self-assembled monolayers with varying wettability

“…5, 6 Our previous work also proved that neural stem cells, MCF-7 breast cancer cell line and mesenchymal stem cells (MSCs) on SAMs were greatly affected by the type of chemical groups terminated on the surfaces. 7–10 …”