Adipose tissue metabolic and inflammatory responses to a mixed meal in lean, overweight and obese men

Travers, Rebecca L.; Motta, Alexandre; Betts, James A.; Thompson, Dylan

doi:10.1007/s00394-015-1087-7

Cited by 17 publications

(24 citation statements)

References 49 publications

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“…As previously reported, serum levels of IL-6 are elevated in obese males16. The presence of IL-6 receptors has been confirmed in male germ cells1718.…”

Section: Resultssupporting

confidence: 76%

IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway

Huang

Miao

Zhang

et al. 2016

Sci Rep

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Zfp637 is a recently identified zinc finger protein, and its functions remain largely unknown. Here, we innovatively demonstrate the effects of Zfp637 on the differentiation of mouse spermatogonia and on its downstream target gene SOX2 in vitro. Obesity has been recognized as a chronic inflammatory disease that leads to decreased sexual function and sexual development disorders. We observed higher levels of IL-6 in serum and testis homogenates from obese mice compared with control mice. We also demonstrated that high levels of IL-6 inhibited Zfp637 expression, and we elucidated the underlying mechanisms. SOCS3 overexpression and STAT3 phosphorylation inhibitor (AG490) were used to investigate the function of the SOCS3/STAT3 pathway during this process. Our results showed that exposure of mouse spermatogonial cells to high levels of IL-6 inhibited Zfp637 expression by increasing SOCS3 expression and inhibiting the phosphorylation of STAT3, further reducing cellular differentiation. Consistent with the in vitro results, we observed increasing expression levels of SOCS3 and SOX2, but a reduction of Zfp637 expression, in obese mouse testes. In conclusion, Zfp637 plays a crucial role in spermatogenesis by downregulating SOX2 expression, and IL-6 can decrease the expression of Zfp637 through the SOCS3/STAT3 signaling pathway.

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“…As previously reported, serum levels of IL-6 are elevated in obese males16. The presence of IL-6 receptors has been confirmed in male germ cells1718.…”

Section: Resultssupporting

confidence: 76%

IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway

Huang

Miao

Zhang

et al. 2016

Sci Rep

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“…A similar result was reported by Jiménez‐Gómez et al (). Adipose and muscle tissues are involved in metabolism of this cytokine and they have shown sensitivity to postprandial stage and dependency on metabolic status, hence, their contributions could explain the observed results (Travers, Motta, Betts & Thompson, ). By contrast, in the MU group, SIO was more effective in IL‐6 expression decreasing in PBMC ( p = 0.017).…”

Section: Discussionmentioning

confidence: 92%

Metabolic status is related to the effects of adding of sacha inchi (Plukenetia volubilis L.) oil on postprandial inflammation and lipid profile: Randomized, crossover clinical trial

2018

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Sacha inchi oil (SIO) is an attractive source of polyunsaturated acids oil. A randomized crossover clinical trial was done to evaluate SIO effects on postprandial lipids and inflammatory state caused by a high‐fat intake. Twenty metabolically healthy (MH) and 22 metabolically unhealthy (MU) subjects consumed a high‐fat breakfast alone or supplemented with SIO. The biomarkers were measured in serum upon fasting, and after 1 and 4 hrs after breakfast. Interleukin‐6 (IL‐6) expression was determined in mononuclear cells. In the MH group, SIO reversed the cholesterol increase [iAUCHFM: 0.27 mmol/L/4 h (IQR: −0.07/0.81); iAUCHFM+S: −0.18 mmol/L/4 h (IQR: −0.49/0.31) p = 0.037] and decreased interleukin‐6 concentration. In MU group, SIO attenuated lipopolysaccharides increase and interleukin‐6 expression [(FCHFM = −1.19 (IQR: −1.72/1.93) and FCHFM+S = −1.83 (IQR: −4,82/−0.01), p = 0.017]. The effects of a high‐fat meal on postprandial lipids and inflammation could be modified by the addition of SIO, but the outcomes are depending on the metabolic individual status. Practical applications The seeds of Plukenetia volubilis L., also known as Sacha inchi, Sacha peanut or Inca peanut are an attractive vegetable source of oil which includes a high content of polyunsaturated fatty acids. Furthermore, the intake of Sacha inchi oil could improve the postprandial responses of a high‐fat intake, and could be able to help to prevent cardiovascular diseases. Our results contribute to know the effects of this oil on postprandial inflammation and lipids. In addition, establishing how a person’s basal metabolic status can determinate the metabolic response to this oil can help improve its use, and our results add evidence about the role of nutrition and diet in health and disease. At this time, the cultivation of Sacha inchi is being proposed as an agro‐industrial alternative for the improvement of quality of living in Colombian rural areas.

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“…Adipose tissue in mammals not only acts as storage for excess of nutrients; it also acts as an endocrine organ secreting adipokines that are involved in a wide range of functions (52)(53)(54)(55). Specifically, obesity is associated with oxidative stress and inflammatory responses in adipose tissue (due to adipocytes hypertrophy and hyperplasia) with consequent increased levels of local and systemic pro-inflammatory cytokines.…”

Section: Resultsmentioning

confidence: 99%

Comparative Circadian Metabolomics Reveal Differential Effects of Nutritional Challenge in the Serum and Liver

Abbondante

Eckel‐Mahan

Ceglia

et al. 2016

Journal of Biological Chemistry

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Diagnosis and therapeutic interventions in pathological conditions rely upon clinical monitoring of key metabolites in the serum. Recent studies show that a wide range of metabolic pathways are controlled by circadian rhythms whose oscillation is affected by nutritional challenges, underscoring the importance of assessing a temporal window for clinical testing and thereby questioning the accuracy of the reading of critical pathological markers in circulation. We have been interested in studying the communication between peripheral tissues under metabolic homeostasis perturbation. Here we present a comparative circadian metabolomic analysis on serum and liver in mice under high fat diet. Our data reveal that the nutritional challenge induces a loss of serum metabolite rhythmicity compared with liver, indicating a circadian misalignment between the tissues analyzed. Importantly, our results show that the levels of serum metabolites do not reflect the circadian liver metabolic signature or the effect of nutritional challenge. This notion reveals the possibility that misleading reads of metabolites in circulation may result in misdiagnosis and improper treatments. Our findings also demonstrate a tissue-specific and time-dependent disruption of metabolic homeostasis in response to altered nutrition.Circadian rhythms govern a large variety of behavioral, physiological, and metabolic processes (1-4). Recent advances reveal that a very large fraction of mammalian metabolism undergoes circadian oscillations (5-12). This notion is critical, and it raises awareness of the need for increased attention to the time of monitoring clinically relevant values in patients. Indeed, studies in humans show that levels of key markers oscillate significantly (13-18), possibly leading to false or misleading reads that may result in questionable therapeutic outcomes. Thus, a comprehensive comparative analysis of the circadian metabolome in the serum versus peripheral tissues is critical to decipher the circulating metabolites that constitute a specific signature of a given physiological state.Circadian rhythms are under the control of clocks that ensure cyclic regulation of a large spectrum of cellular and molecular mechanisms. In mammals, the central clock is located in the suprachiasmatic nucleus (SCN) 2 of the anterior hypothalamus. The SCN integrates external daily cues, such as the light-dark cycle, and operates as a synchronizer for a multitude of peripheral clocks located in most tissues (19 -21). Peripheral clocks respond to nutritional cues and can be uncoupled from the SCN by restricted feeding (9,(22)(23)(24). Recent studies have shown that restriction of the time of feeding (9) as well as nutritional challenge by a high fat diet (HFD) (24 -26) result in extensive modifications of liver metabolism. Furthermore, the liver clock displays a highly dynamic homeostasis associated with an elaborate reprogramming of its molecular gears under nutritional challenge (26). Accumulating evidence underscores the intimate interplay between ...

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Adipose tissue metabolic and inflammatory responses to a mixed meal in lean, overweight and obese men

Cited by 17 publications

References 49 publications

IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway

IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway

Metabolic status is related to the effects of adding of sacha inchi (Plukenetia volubilis L.) oil on postprandial inflammation and lipid profile: Randomized, crossover clinical trial

Comparative Circadian Metabolomics Reveal Differential Effects of Nutritional Challenge in the Serum and Liver

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