Adipose Tissue Model Using Three-Dimensional Cultivation of Preadipocytes Seeded onto Fibrous Polymer Scaffolds

Kang, Xihai; Xie, Yizhen; Kniss, Douglas A.

doi:10.1089/ten.2005.11.458

Cited by 71 publications

(55 citation statements)

References 47 publications

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“…The open, porous structure acts as a preserver of space for efficient cellular adhesion and seeding and facilitates vessel ingrowth with subsequent mass transport of oxygen and nutrients. The three-dimensional geometry of the native ECM constrains cells during regeneration and provides space for tissue development [25] and in vitro studies have shown that the scaffold geometry promotes preadipocyte differentiation [31]. The architecture of adipose tissue has been reconstructed using adipose-derived stromal cells seeded onto different scaffold materials [6][7][8]13,14].…”

Section: Discussionmentioning

confidence: 99%

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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a b s t r a c tHistioconductive approaches to soft-tissue defects use scaffolds seeded with lineage-and tissue-specific progenitors to generate tissue which should reside in equilibrium with adjacent tissue. Scaffolds guide histiogenesis by ensuring cell-cell and cell-matrix interactions. Hyaluronic acid-based (HA) preadipocyte-seeded scaffolds were evaluated for their adipo-conductive potential and efficacy in humans. Preadipocytes were isolated from lipoaspirate material and seeded on HA scaffolds. The cellular biohybrid (ADIPOGRAFT Ò ) and an acellular control scaffold (HYAFF Ò 11) were implanted subcutaneously. At specific time points (2, 8 and 16 weeks) explants were analyzed histopathologically with immunohistochemistry. No adverse tissue effects occurred. Volume loss and consistent degradation of the HYAFF Ò 11 scaffolds compared to the ADIPOGRAFT Ò group indicated progressive tissue integration. No consistent histological differences between both groups were observed. By 8 weeks all void spaces within the scaffolds were filled with cells with pronounced matrix deposition in the ADIPOGRAFT Ò bio-hybrids. Here we show that HA scaffolds were stable cell carriers and had the potential to generate volumeretaining tissue. However, no adipogenic differentiation was observed within the preadipocyte-seeded scaffolds.

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Section: Discussionmentioning

confidence: 99%

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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“…Recently, cell-based approaches utilizing adipogenic progenitor cells in combination with biomaterial carriers for fat tissue engineering have been developed and were reported to promote both short-term in vivo adipogenesis and to repair defect sites [8][9][10][11][12][13][14]. For these cellbased applications, human mesenchymal stem cells derived from bone marrow (hMSCs) or human adipose tissue (hASCs) have both been suggested as potential cell sources for adipose repair therapies [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…For these cellbased applications, human mesenchymal stem cells derived from bone marrow (hMSCs) or human adipose tissue (hASCs) have both been suggested as potential cell sources for adipose repair therapies [13][14][15][16]. hMSCs and hASCs are readily isolatable from bone marrow aspirates or adipose stromal vascular fractions, respectively, and can differentiate into a variety of mesenchymal lineages.…”

Section: Introductionmentioning

confidence: 99%

“…hMSCs and hASCs are readily isolatable from bone marrow aspirates or adipose stromal vascular fractions, respectively, and can differentiate into a variety of mesenchymal lineages. Both these properties are desirable attributes for incorporation into clinical repair modalities [13,14,16]. Currently, however the efficacy of exogenously-delivered stem cell populations to support the generation of long-term volume stable adipose tissue in vivo is limited by suboptimal properties of their biomaterial carriers including insufficient biocompatibility and rapid scaffold degradation rates [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds

et al. 2007

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Biomaterials derived from silk fibrion prepared by aqueous (AB) and organic (HFIP) solvent based processes, along with collagen (COL) and poly-lactic acid (PLA) based scaffolds were studied in vitro and in vivo for their utility in adipose tissue engineering strategies. For in vitro studies, human bone marrow and adipose-derived mesenchymal stem cells (hMSCs and hASCs) were seeded on the various biomaterials and cultured for 21 days in the presence of adipogenic stimulants (AD) or maintained as noninduced controls. Alamar Blue analysis revealed each biomaterial supported initial attachment of hMSCs and hASCs to similar levels for all matrices except COL in which higher levels were observed. hASCs and hMSCs cultured on all biomaterials in the presence of AD showed significant upregulation of adipogenic mRNA transcript levels (LPL, GLUT4, FABP4, PPARγ, adipsin, ACS) to similar extents when compared to noninduced controls. Similarly Oil-Red O analysis of hASC or hMSC-seeded scaffolds displayed substantial amounts of lipid accumulating adipocytes following cultivation with AD. The data revealed AB and HFIP scaffolds supported similar extents of lipid accumulating cells while PLA and COL scaffolds qualitatively displayed lower and higher extents by comparison, respectively. Following a 4 week implantation period in a rat muscle pouch defect model, both AB and HFIP scaffolds supported in vivo adipogenesis either alone or seeded with hASCs or hMSCs as assessed by Oil-Red O analysis, however the presence of exogenous cell sources substantially increased the extent and frequency of adipogenesis observed. In contrast, COL and PLA scaffolds underwent rapid scaffold degradation and were irretrievable following the implantation period. The results suggest that macroporous 3D AB and HFIP silk fibroin scaffolds offer an important platform for cell-based adipose tissue engineering applications, and in particular, provide longer-term structural integrity to promote the maintenance of soft tissue in vivo.

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“…In addition, the use of adipogenic progenitor cells derived from hTGSCs may also emerge as a promising approach for cell-based adipose tissue engineering with various clinical implications: fatty defects are usually seen after traumas, deep burns, tumor resections such as mastectomies, and are important in cosmetic facial problems involving the cheek, chin, and jaw. [35][36][37] In contrast, P85 had no significant impact on the fatty acid profile of hTGSCs; however, it displayed considerable cytotoxic effect during differentiation even at low concentrations (0.01%, 0.05%). P85 is generally used for the inhibition of the Pgp drug efflux system, which is crucial for improved delivery of Pgp-dependent molecules through the blood-brain barrier, intestinal tight-junctions, and MDR-tumors.…”

mentioning

confidence: 90%

Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers

Doğan¹,

Yalvaç²,

Şahın³

et al. 2012

IJN

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Stem cell usage provides novel avenues of tissue regeneration and therapeutics across disciplines. Apart from ethical considerations, the selection and amplification of donor stem cells remain a challenge. Various biopolymers with a wide range of properties have been used extensively to deliver biomolecules such as drugs, growth factors and nucleic acids, as well as to provide biomimetic surface for cellular adhesion. Using human tooth germ stem cells with high proliferation and transformation capacity, we have investigated a range of biopolymers to assess their potential for tissue engineering. Tolerability, toxicity, and their ability to direct differentiation were evaluated. The majority of pluronics, consisting of both hydrophilic and hydrophobic poly(ethylene oxide) chains, either exerted cytotoxicity or had no significant effect on human tooth germ stem cells; whereas F68 increased the multi-potency of stem cells, and efficiently transformed them into osteogenic, chondrogenic, and adipogenic tissues. The data suggest that differentiation and maturation of stem cells can be promoted by selecting the appropriate mechanical and chemical properties of polymers. It has been shown for the first time that F68, with its unique molecular characteristics, has a great potential to increase the differentiation of cells, which may lead to the development of new tissue engineering strategies in regenerative medicine.

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Adipose Tissue Model Using Three-Dimensional Cultivation of Preadipocytes Seeded onto Fibrous Polymer Scaffolds

Cited by 71 publications

References 47 publications

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds

Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers

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