Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål

Jiang, Yiping; Li, Lei; Liu, Zongyu; You, Linlin; Wu, You; Xu, Bing; Ge, Lin-Quan; Song, Qisheng; Wu, Jin-Cai

doi:10.1038/srep18984

Cited by 15 publications

(10 citation statements)

References 32 publications

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“…In the present study, the expression level of atgl gene did not show any change between the conventional and germ-free zebrafish but it was changed in the liver and intestine when zebrafish were treated with antibiotics. Atgl mediated the antibiotics stimulated reproduction in the brown planthopper ( Jiang et al, 2016 ), which is consistent to the present results. The two carnitine palmitoyltransferases, cpt1a and cpt1b , involved in mitochondrial fatty acid oxidation were upregulated in germ-free zebrafish ( Rawls et al, 2004 ), while in the present study, we did not find a similar trend.…”

Section: Discussionsupporting

confidence: 93%

The Presence or Absence of Intestinal Microbiota Affects Lipid Deposition and Related Genes Expression in Zebrafish (Danio rerio)

Ren

Limbu

et al. 2018

Front. Microbiol.

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Understanding how intestinal microbiota alters energy homeostasis and lipid metabolism is a critical process in energy balance and health. However, the exact role of intestinal microbiota in the regulation of lipid metabolism in fish remains unclear. Here, we used two zebrafish models (germ-free and antibiotics-treated zebrafish) to identify the role of intestinal microbiota in lipid metabolism. Conventional and germ-free zebrafish larvae were fed with egg yolk. Transmission electron microscopy was used to detect the presence of lipid droplets in the intestinal epithelium. The results showed that, microbiota increased lipid accumulation in the intestinal epithelium. The mRNA sequencing technology was used to assess genes expression level. We found majority of the differentially expressed genes were related to lipid metabolism. Due to the limitation of germ-free zebrafish larvae, antibiotics-treated zebrafish were also used to identify the relationship between the gut microbiota and the host lipid metabolism. Oil-red staining showed antibiotics-treated zebrafish had less intestinal lipid accumulation than control group. The mRNA expression of genes related to lipid metabolism in liver and intestine was also quantified by using real-time PCR. The results indicated that apoa4, hsl, cox15, slc2a1a, and lss were more related to intestinal bacteria in fish, while the influence of intestinal microbiota on the activity of fabp6, acsl5, cd36, and gpat2 was different between the liver and intestine. This study identified several genes regulated by intestinal microbiota. Furthermore, the advantages and disadvantages of each model have been discussed. This study provides valuable information for exploring host-microbiota interactions in zebrafish in future.

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Section: Discussionsupporting

confidence: 93%

The Presence or Absence of Intestinal Microbiota Affects Lipid Deposition and Related Genes Expression in Zebrafish (Danio rerio)

Ren

Limbu

et al. 2018

Front. Microbiol.

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“…A dicer1 gene, which is mostly responsible for micro‐RNA precursor processing, was demonstrated to be essential for oocyte maturation in the telotrophic ovary (Zhang et al ., ). An adipose triglyceride lipase gene was reported to participate in ovarian development and oviposition activity in female adults (Jiang et al ., ). We recently identified a female‐specific Bicaudal‐C gene that is vital in oogenesis (Zhang et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Pancreatic lipase‐related protein 2 is essential for egg hatching in the brown planthopper, Nilaparvata lugens

Huang

Zhou

et al. 2016

Insect Molecular Biology

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In this study, we identified a pancreatic lipase-related protein 2 (PLRP2) gene through searching the transcriptome data of the brown planthopper, Nilaparvata lugens, a monophagous rice pest. PLRP2 mRNAs were first isolated in the human pancreas and play an important role in hydrolysis of galactolipids from the diet. Although homologous PLRP2 genes have been identified in many insect species, their physiological functions remain unknown. The present study for the first time reports the functional role of PLRP2 in an insect species. Differing from mammal PLRP2s, N. lugens PLRP2 was highly expressed in developing oocytes of the ovaries of female adults and little expressed in laid eggs. Suppression of N. lugens PLRP2 expression using RNA interference significantly inhibited egg hatching in rice seedlings, implying that N. lugens PLRP2 was important for oocyte maturation and development. This novel finding will improve our understanding of the reproductive strategies in insects and provides a potential target for future management of crop pests.

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“…JGM increased BPH fecundity via increased fatty acid metabolism (Jiang et al, 2016; Li et al, 2016), however, the broad impact of JGM led us to consider additional pathways that might also influence BPH reproduction. From digital gene (DEG) expression profiles, we identified a UDP-glycosyltransferase 1-2 like ( UGT12 ) gene that was up-regulated in adult females following foliar JGM treatments (200 ppm) at 2 days after emergence (2 DAE).…”

Section: Introductionmentioning

confidence: 99%

Jinggangmycin-Induced UDP-Glycosyltransferase 1-2-Like Is a Positive Modulator of Fecundity and Population Growth in Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Sui

et al. 2019

Front. Physiol.

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The antibiotic jinggangmycin (JGM) is broadly applied in Chinese rice producing regions to control rice blight, a fungal disease. Aside from protecting rice plants from the disease, JGM leads to the unexpected action of stimulating brown planthopper (BPH; Nilaparvata lugens ; Hemiptera: Delphacidae) reproduction to the extent it can influence population sizes. The JGM-induced BPH population growth has potential for severe agricultural problems and we are working to understand and mitigate the mechanisms of the enhanced reproduction. UDP-glucuronosyltransferases (UGTs) are multifunctional detoxification enzymes responsible for biotransformation of diverse lipophilic compounds. The biological significance of this enzyme family in insect fecundity is not fully understood, however, upregulated UGT12 in JGM-treated BPH, may influence fecundity through metabolism of developmental hormones. This idea prompted our hypothesis that NlUGT12 is a positive modulator of BPH reproductive biology. JGM treatment led to significant increases in accumulations of mRNA encoding Nl UGT12, numbers of eggs laid, oviposition period, juvenile hormone III titers, and fat body, and ovarian protein contents. dsUGT12 treatment suppressed NlUGT12 expression and reversed JGM-enhanced effects, resulting in under-developed ovaries and reduced expression of juvenile hormone acid methyltransferase and the JH receptor, methoprene tolerant. Application of the JH analog, methoprene, on dsUGT12 treated-females partially reversed the dsUTG12 influence on vitellogenin synthesis and on NlUGT12 expression. These results represent an important support for our hypothesis.

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Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål

Cited by 15 publications

References 32 publications

The Presence or Absence of Intestinal Microbiota Affects Lipid Deposition and Related Genes Expression in Zebrafish (Danio rerio)

The Presence or Absence of Intestinal Microbiota Affects Lipid Deposition and Related Genes Expression in Zebrafish (Danio rerio)

Pancreatic lipase‐related protein 2 is essential for egg hatching in the brown planthopper, Nilaparvata lugens

Jinggangmycin-Induced UDP-Glycosyltransferase 1-2-Like Is a Positive Modulator of Fecundity and Population Growth in Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

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