Adjuvant activities of CTAB-modified Polygonatum sibiricum polysaccharide cubosomes on immune responses to ovalbumin in mice

Liu, Zhenguang; Ni, Haiyu; Lin, Yan; Xu, Shuwen; Bo, Ruonan; Qiu, Tianxin; Gu, Peng; Zhu, Tianyu; He, Jin; Wusiman, Adelijiang; Zhu, Shaowu; Liu, Jiaguo; Hu, Yuanliang; Wang, Deyun

doi:10.1016/j.ijbiomac.2020.01.174

Cited by 43 publications

(24 citation statements)

References 34 publications

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“…Cytokines generally regulate cell growth, differentiation and immune responses by binding to specific receptors and thereby modulating adaptive and innate immunity. [36][37][38] Th1 and Th2 cells are crucial parts of the immune system and maintain equilibrium in the body. Th1 cells primarily secrete IFN-γ, IL-2 and TNF-⊍, whereas Th2 cells mainly produce IL-6, IL-4 and IL-10.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of immune responses using ovalbumin‐conjugatedEucommia ulmoides leaf polysaccharides encapsulated in a cubic liquid‐crystalline phase delivery system

et al. 2022

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BACKGROUND: To improve the adjuvant activity of polysaccharides from Eucommia ulmoides leaves (PsEUL) in inducing an effective immune response against ovalbumin (OVA), PsEUL were conjugated to OVA using the N-(3-dimethylaminopropyl)-N 0 -ethylcarbodiimide hydrochloride (EDC) method. The synthesized PsEUL-OVA was encapsulated using phytantriol and F127 to produce PsEUL-OVA cubosomes (Cubs), a novel delivery system. The physicochemical properties and immune modulation effects of this novel delivery system were explored. RESULTS:In vitro, PsEUL-OVA/Cubs carrying large amounts of OVA were rapidly phagocytized by macrophages and upregulated macrophage proliferation, thereby stimulating cytokine production (interleukin (IL)-6 and IL-4). In vivo, PsEUL-OVA/Cubs increased the titer of OVA-specific antibodies (immunoglobulin (Ig)G, IgG2b, IgG2a and IgG1) and cytokine levels (IL-2, IL-6, IL-4 and interferon-γ). In addition, the cubosomes promoted the differentiation of CD8 + and CD4 + T cells in the spleen and the maturation of dendritic cells (DCs). These results indicated that PsEUL-OVA/Cubs stimulated both cellular and humoral immune responses by enhancing the phagocytic activity of DCs and macrophages and increasing the antigen presentation efficiency. CONCLUSION: Collectively, the findings demonstrate that PsEUL-antigen/Cubs can be a useful delivery vehicle with immune response-promoting effects. Therefore, this study lays the foundation for the development of novel adjuvant-antigen delivery systems with potential applications in vaccine design.

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Section: Discussionmentioning

confidence: 99%

Enhancement of immune responses using ovalbumin‐conjugatedEucommia ulmoides leaf polysaccharides encapsulated in a cubic liquid‐crystalline phase delivery system

et al. 2022

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“…CD8 is also a Th cell TCR; CD8 + T cells differentiate into CTL after activation and can specifically kill target cells. Therefore, the CD4 + T/CD8 + T ratio is used clinically to reflect the activity of T lymphocytes [ 66 ]. Huang et al demonstrated that the Ag85B-HspX fusion protein (AH) conjugated with Arabinogalactan-poly(I:C) (AG-P) to generate a vaccine (AH-AG-P) can significantly increase the proliferation and differentiation of CD8 + T cells in C57BL/6 mice [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Eucommia ulmoides Leaf Polysaccharide in Conjugation with Ovalbumin Act as Delivery System Can Improve Immune Response

et al. 2021

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In this investigation, to maximize the desired immunoenhancement effects of PsEUL and stimulate an efficient humoral and cellular immune response against an antigen, PsEUL and the model antigen ovalbumin (OVA) were coupled using the N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) reaction to yield a novel delivery system (PsEUL-OVA). The physicochemical characteristics and immune regulation effects of this new system were investigated. We found the yield of this EDC method to be 46.25%. In vitro, PsEUL-OVA (200 μg mL−1) could enhance macrophage proliferation and increase their phagocytic efficiency. In vivo, PsEUL-OVA could significantly increase the levels of OVA-specific antibody (IgG, IgG1, IgG2a, and IgG2b) titers and cytokine (IL-2, IL-4, IL-6, IFN-γ) levels. Additionally, it could activate T lymphocytes and facilitate the maturation of dendritic cells (DCs). These findings collectively suggested that PsEUL-OVA induced humoral and cellular immune responses by promoting the phagocytic activity of macrophages and DCs. Taken together, these results revealed that PsEUL-OVA had the potential to improve immune responses and provide a promising theoretical basis for the design of a novel delivery system.

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“…RES suppressed enterovirus replication and reduced the virus’s generation of IL-6 and TNF-α [ 48 ]. RES increased CD4+ cell proliferation, splenic lymphocyte proliferation, and peritoneal macrophage activity in mice [ 49 ]. Intravenous RES therapy decreases ischemia-induced inflammation and oxidants produced by HX/XO, but not leukotriene B4 [ 50 ].…”

Section: Res—a General Concept and A Basic Outlinementioning

confidence: 99%

Involvement of Resveratrol against Brain Cancer: A Combination Strategy with a Pharmaceutical Approach

et al. 2022

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A brain tumor (BT) is a condition in which there is growth or uncontrolled development of the brain cells, which usually goes unrecognized or is diagnosed at the later stages. Since the mechanism behind BT is not clear, and the various physiological conditions are difficult to diagnose, the success rate of BT is not very high. This is the central issue faced during drug development and clinical trials with almost all types of neurodegenerative disorders. In the first part of this review, we focus on the concept of brain tumors, their barriers, and the types of delivery possible to target the brain cells. Although various treatment methods are available, they all have side effects or toxic effects. Hence, in the second part, a correlation was made between the use of resveratrol, a potent antioxidant, and its advantages for brain diseases. The relationship between brain disease and the blood–brain barrier, multi-drug resistance, and the use of nanomedicine for treating brain disorders is also mentioned. In short, a hypothetical concept is given with a background investigation into the use of combination therapy with resveratrol as an active ingredient, the possible drug delivery, and its formulation-based approach.

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Adjuvant activities of CTAB-modified Polygonatum sibiricum polysaccharide cubosomes on immune responses to ovalbumin in mice

Cited by 43 publications

References 34 publications

Enhancement of immune responses using ovalbumin‐conjugatedEucommia ulmoides leaf polysaccharides encapsulated in a cubic liquid‐crystalline phase delivery system

Enhancement of immune responses using ovalbumin‐conjugatedEucommia ulmoides leaf polysaccharides encapsulated in a cubic liquid‐crystalline phase delivery system

Eucommia ulmoides Leaf Polysaccharide in Conjugation with Ovalbumin Act as Delivery System Can Improve Immune Response

Involvement of Resveratrol against Brain Cancer: A Combination Strategy with a Pharmaceutical Approach

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