Adlay Seed Extract (<i>Coix lachryma-jobi</i>L.) Decreased Adipocyte Differentiation and Increased Glucose Uptake in 3T3-L1 Cells

Ha, Do Thi; Trung, Trân Nam; Thu, Nguyen Bich; Ơn, Trần Văn; Nam, Nguyen Hai; Men, Chu Van; Phượng, Trần Thị; Bae, KiHwan

doi:10.1089/jmf.2010.1155

Cited by 34 publications

(12 citation statements)

References 58 publications

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“…Moreover, activation of AMPK was previously associated with the inhibition of adipocyte differentiation [70] or increased glucose uptake. Indeed, our data are in an agreement with recent reports by Ha et al [71,72] demonstrating an increase of glucose uptake and inhibition of adipocyte differentiation through the activation of AMPK in 3T3-L1 cells.…”

Section: Discussionsupporting

confidence: 94%

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Thyagarajan‐Sahu

Lane

Slíva

2011

BMC Complement Altern Med

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BackgroundObesity is a health hazard which is closely associated with various complications including insulin resistance, hypertension, dyslipidemia, atherosclerosis, type 2 diabetes and cancer. In spite of numerous preclinical and clinical interventions, the prevalence of obesity and its related disorders are on the rise demanding an urgent need for exploring novel therapeutic agents that can regulate adipogenesis. In the present study, we evaluated whether a dietary supplement ReishiMax (RM), containing triterpenes and polysaccharides extracted from medicinal mushroom Ganoderma lucidum, affects adipocyte differentiation and glucose uptake in 3T3-L1 cells.Methods3T3-L1 pre-adipocytes were differentiated into adipocytes and treated with RM (0-300 μg/ml). Adipocyte differentiation/lipid uptake was evaluated by oil red O staining and triglyceride and glycerol concentrations were determined. Gene expression was evaluated by semi-quantitative RT-PCR and Western blot analysis. Glucose uptake was determined with [3H]-glucose.ResultsRM inhibited adipocyte differentiation through the suppresion of expression of adipogenic transcription factors peroxisome proliferator-activated receptor-γ (PPAR-γ), sterol regulatory element binding element protein-1c (SREBP-1c) and CCAAT/enhancer binding protein-α (C/EBP-α). RM also suppressed expression of enzymes and proteins responsible for lipid synthesis, transport and storage: fatty acid synthase (FAS), acyl-CoA synthetase-1 (ACS1), fatty acid binding protein-4 (FABP4), fatty acid transport protein-1 (FATP1) and perilipin. RM induced AMP-activated protein kinase (AMPK) and increased glucose uptake by adipocytes.ConclusionOur study suggests that RM can control adipocyte differentiation and glucose uptake. The health benefits of ReishiMax warrant further clinical studies.

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Section: Discussionsupporting

confidence: 94%

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Thyagarajan‐Sahu

Lane

Slíva

2011

BMC Complement Altern Med

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“…Some researchers found that bioactivity compounds extracted from Adlay seed manifested inhibitory effects on melanin production and cellular oxygen stress in B16F10 melanoma cells (Huang, Hsieh, Niu, & Chang, 2014). The ethyl acetate fraction of an ethanol extract of C. lachryma-jobi, which included gallicacid, chlorogenic acid, caffeic acid, and ferulic acid, decreased adipocyte differentiation and increased glucose uptake in 3T3-L1 cells (Ha et al, 2010). Taking Coix seeds tablets was beneficial to increase the percentages of CD16 + , CD57 + (mature, most active natural killer [NK] cells) and CD3 + , CD56 + (major histocompatibility complex-no restricted cytotoxic T cells) from healthy volunteers (Hidaka, Kaneda, Amino, & Miyai, 1992).…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

et al. 2016

CyTA - Journal of Food

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2017)Immunomodulatory activity of small molecular (≤3 kDa) Coix glutelin enzymatic hydrolysate, CyTA -Journal of Food, 15:1, 41-48, ABSTRACT In this study, small molecular (≤3 kDa) Coix glutelin hydrolysate (CGH) was obtained by the method of enzymatic hydrolysis and then immunomodulatory activity was evaluated in vitro and in vivo. Stimulation index of mice splenocytes was increased in a dose-dependent manner after treated with 3.125-25 μg/mL CGH. Acid phosphatase in mice peritoneal macrophages was significantly improved after stimulated with CGH at some concentration. The CGH stimulated the secretion of NO from Raw264.7 in a concentration-dependent manner and also significantly inhibited lipopolysaccharideinduced cell excessive activation at some extent. After successive administration of CGH for 21 days, the spleen index of ICR was significantly increased to 3.73 mg/g for the group administrated 800 mg/kg CGH. Malondialdehyde (MDA) assay displayed that 200 mg/kg CGH increased mice serum MDA concentration, while the variation was obsolete in the other CGH groups. All results demonstrated that CGH took essential effects on immunomodulatory activities.Actividad inmunomoduladora de hidrolisato glutelina encimático de moléculas pequeñas de Coix (≤ 3 kDa) RESUMEN En este estudio, se obtuvo hidrolisato glutelina de Coix (CGH) de molécula pequeña (≤ 3 kDa) mediante el método de hidrólisis enzimática y después se evaluó la actividad sinmunomoduladora in vitro y in vivo. El índice de estimulación (SI) de los esplenocitos de ratón aumentó de forma dependiente a la dosis después ser tratados con 3,125-25 μg/mL de CGH. La fosfatasa ácida (ACP) en los macrófagos peritoneales de ratón mejoró significativamente después de que se estimulara con CGH en algunas concentraciones. El CGH estimuló la secreción de NO a partir de Raw264.7 de forma dependiente de la concentración e inhibió de forma significativa la activación excesiva de células inducidas con LPS en cierta medida. Después de una administración sucesiva de CGH durante 21 días, el índice esplénico de ICR aumentó significativamente a 3,73 mg/g para el grupo al cual se había administrado 800 mg/kg de CGH. El ensayo de malondialdehído (MDA) mostró que los 200 mg/kg de CGH hicieron aumentar la concentración de MDA del suero de ratón, mientras que la variación fue obsoleta en los otros grupos de CGH. Todos los resultados demostraron que CGH obtuvo efectos esenciales en las actividades inmunomoduladoras. ARTICLE HISTORY

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“…Hanjeli (Coix lacryma-jobi L.) adalah salah satu jenis tanaman serealia potensial yang memiliki kandungan karbohidrat tinggi, protein, lemak nabati, kalsium, dan senyawa lain yang berguna dalam mengobati berbagai macam penyakit (Nurmala, 2010;Ha et al, 2010). Dengan demikian peranannya tidak saja untuk keperluan diversifikasi pangan, tetapi juga sebagai bahan baku untuk keperluan medis.…”

Section: Pendahuluanunclassified

Pengaruh invigorasi benih hanjeli (Coix lacryma-jobi L.) terdeteriorasi terhadap mutu fisiologis serta dampaknya terhadap hasil

Sumadi¹,

Nurmala²

2019

kultivasi

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Sumadi dan T. Nurmala : Pengaruh invigorasi benih hanjeli (Coix lacryma-jobi L.) terdeteriorasi terhadap mutu fisiologis serta dampaknya terhadap hasil Sumadi • T. Nurmala Pengaruh invigorasi benih hanjeli (Coix lacryma-jobi L.) terdeteriorasi terhadap mutu fisiologis serta dampaknya terhadap hasilThe effect of invigoration deteriorated job's tears seeds to physiological quality and its impact on yield Abstract. Seed invigoration is an effort to improve the quality of deteriorated seeds by various growth regulators, pesticides enriched with hormones, and biological agents. The objective of the study was to determine the appropriate substance for quality enhancement of deteriorated job's tears seeds and their impact on yield. High seed quality is one of the factors affected growth and yield. The experiment was conducted from November 2015 to June 2016 at the Experimental Stations, Faculty of Agriculture, University of Padjadjaran, Jatinangor, Sumedang. Experiments designed by split plot design that replicated three times. The main plot was two levels of deterioration of seeds, there were medium vigor seeds (germination capacity (GC) ± 80% ); deteriorated seeds or low vigor (GC ± 50%). Subplot consisted of five levels of invigoration treatment, there were control; soaking the seeds in 70 0 C hot water for 30 minutes; coating of seeds with thiametoxam; soaking the seeds in instant gibberellic acid (GA) and coating the seeds with Trichoderma compost solution. The results of the experiment showed that there was an interaction effect between the level of seed deterioration and seed invigoration as measured by seedling emergence potential, but no affected on yield components and seed weight per plant. Invigoration with GA and Trichoderma able to vigour increased of deteriorated seeds

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Adlay Seed Extract (Coix lachryma-jobiL.) Decreased Adipocyte Differentiation and Increased Glucose Uptake in 3T3-L1 Cells

Cited by 34 publications

References 58 publications

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

Pengaruh invigorasi benih hanjeli (Coix lacryma-jobi L.) terdeteriorasi terhadap mutu fisiologis serta dampaknya terhadap hasil

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