Adsorption and desorption behavior of basic proteins on zeolites

Matsui, Masanao; Kiyozumi, Yoshimichi; Mizushina, Yoshiyuki; Sakaguchi, Kengo; Mizukami, Fujio

doi:10.1016/j.seppur.2015.05.023

Cited by 28 publications

(7 citation statements)

References 37 publications

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“…Zeolites can adsorb denatured proteins with co‐existing GdnHCl at a high concentration, and desorb the proteins by the addition of PEG molecules . In the present study, we have used two types of zeolites, zeolite L and zeolite beta, because of two reasons: (a) it was reported that proteins adsorbed on the zeolite beta desorb efficiently by the addition of PEG molecules, and (b) we tried to evaluated the effect of the Si/Al ratio on protein desorption efficacy (the Si/Al ratio of zeolite L was generally lower than zeolite beta). The Si/Al ratio of zeolites, an indicator of their hydrophobicity, affects the adsorption of proteins on zeolites, because the hydrophobic interaction is attributable to the adsorption.…”

Section: Resultsmentioning

confidence: 99%

A zeolite as a tool for successful refolding of PEGylated proteins and their reassembly with tertiary structures

Sonotaki

Noguchi

Yohda

et al. 2019

Biotechnology Progress

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In the present study, we demonstrated zeolites' potential contribution to establish a method for preparing successfully refolded and reassembled PEGylated protein nanoparticles without the use of protein denaturants through the proteins' reassembly process. At first, the PEGylated nanoparticles are disassembled into identical PEGylated protein subunits by means of protein denaturants, and then the denatured subunits are adsorbed to zeolites. After the complete removal of denaturants, high‐molecular‐weight poly(ethylene glycol) (PEG) molecules are added to a solution where the zeolites suspend. Consequently, the PEGylated proteins are gradually reassembled into nanoparticles because the subunits are desorbed from the zeolites by the steric hindrance of the added PEG molecules. The present study reveals that PEGylated encapsulin was reassembled and hollow encapsulin nanoparticles were obtained. The results clearly demonstrate the usefulness of zeolites as a tool for the successful refolding of PEGylated proteins and their reassembly with tertiary structures.

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Section: Resultsmentioning

confidence: 99%

A zeolite as a tool for successful refolding of PEGylated proteins and their reassembly with tertiary structures

Sonotaki

Noguchi

Yohda

et al. 2019

Biotechnology Progress

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“…It was also reported that a variation in the SiO 2 /Al 2 O 3 ratio of the natural zeolites affects the hydrophilicity/hydrophobicity of this material, which is correlated with the adsorption, catalysis, and ion exchange properties [18,19]. Zeolite dealumination using acid as a chemical agent allows zeolites with a higher SiO 2 /Al 2 O 3 ratio to be obtained, which are usually used for the removal of organic hydrophobic contaminants in water [20] and volatile organic compounds in air [21]; whereas alkali treated zeolite using organic hydroxides [22] or sodium hydrate [16,23] can achieve materials with a lower SiO 2 /Al 2 O 3 ratio for the removal of ammonium [24] and heavy metals [6,25].…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies [16,18,19,22,23,[25][26][27][28][29][30], the researches focused mainly on the effect of the SiO 2 /Al 2 O 3 ratio on the structural properties of zeolites. Moreover, these studies used synthesized zeolites [19,22,[26][27][28]30], whereas the only raw zeolites used being mordenite and clinoptilolite. Kussainova et al [31] reported the Pb(II) removal from an acid media.…”

Section: Introductionmentioning

confidence: 99%

Sorption of Pb(II) from Aqueous Solutions by Acid-Modified Clinoptilolite-Rich Tuffs with Different Si/Al Ratios

et al. 2019

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The removal of Pb(II) from aqueous solutions by acid-modified clinoptilolite-rich tuff was investigated in this work. Clinoptilolite-rich tuff samples were treated using H2SO4 at different concentrations. Prior to and following acid treatment, the samples were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and Fourier-transform infrared spectroscopy (FTIR). The pH of the point of zero charge (pHPZC) was also determined as part of this characterization. Batch studies were studied to investigate Pb(II) removal as a function of contact time, initial Pb(II) concentration, adsorbent dosage, and solution pH. The results of the XRD and SEM techniques showed that clinoptilolite is the main mineral of the non- and acid-treated natural zeolite samples. However, EDS analysis indicated that the Si/Al ratio increases as the exchangeable ions decrease with increasing acid concentrations. The optimum conditions for Pb(II) removal for samples with 4.37 ≤ Si/Al ≤ 7.9 were found to be as follows: Contact time of 60–360 min, pH: 6–8, and adsorbent dose of 6 mg g−1; whereas for acid-modified clinoptilolite-rich tuffs with 9.01 ≤ Si/Al ≤ 9.52, these conditions were as follows: Contact time of 1440 min, pH: 8–10, and adsorbent dose of 10 mg g−1. The experimental data were analyzed by kinetic and isotherms models. The results showed that the sorption of Pb(II) on samples with Si/Al ratios of 4.37, 5.31, and 7.91 were in agreement with the pseudo-second order and Langmuir isotherm with qm = 48.54, 37.04, and 14.99 mg g−1, respectively, while the kinetic data and isotherm for samples with 9.01 ≤ Si/Al ≤ 9.52 were found to fit the pseudo-first order and Freundlich model.

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“…The structure of natural zeolite is investigated by different physical and chemical methods (electron microscopic, X-ray, IR-spectroscopy), by means of which physical characteristics, porosity, specific surface, pore sizes and other properties are determined [3,4]. The most of the investigations is devoted to the sorption of inorganic substances, mainly metals [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Statics and Kinetics of Albumin Adsorption by Natural Zeolite

Hyvlud¹,

Sabadash²,

Гумницький³

et al. 2019

ChChT

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The statics and kinetics of albumin adsorption on natural zeolite of clinoptilolite structure have been investigated. The basic characteristics of zeolite have been determined. The method of albumin in a solution analysis was given. An isotherm of albumin adsorption was plotted and the Langmuir equation describing this isotherm was given. The presence of an external and pore diffusion adsorption mechanism has been experimentally established during the study of kinetics in a machine with mechanical stirring. The mass-transfer coefficient for external diffusion and the molecular diffusion constant for the pore diffusion are determined.

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Adsorption and desorption behavior of basic proteins on zeolites

Cited by 28 publications

References 37 publications

A zeolite as a tool for successful refolding of PEGylated proteins and their reassembly with tertiary structures

A zeolite as a tool for successful refolding of PEGylated proteins and their reassembly with tertiary structures

Sorption of Pb(II) from Aqueous Solutions by Acid-Modified Clinoptilolite-Rich Tuffs with Different Si/Al Ratios

Statics and Kinetics of Albumin Adsorption by Natural Zeolite

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