Adsorption induced enzyme denaturation: The role of protein surface in adsorption induced protein denaturation on allyl glycidyl ether (AGE)–ethylene glycol dimethacrylate (EGDM) copolymers

Immobilization of pectinase on reusable polymer support for clarification of apple juice

“…Pectinases are, in general, a group of monomeric glycoproteins [33] with molecular weights ranging from 30 kDa to 65 kDa, pH optimum between 3.5 to 5 and isoelectic point in the range 3.0-6.0 [34]. Our earlier studies on binding of glycoproteins such as glucose dehydrogenase and alkaline phosphatase to epoxy-activated polymer have shown that glycoproteins are not easily bound to neutral polymeric supports due to lack of hydrophobic interactions between protein and polymer surfaces [35]. In the case of ionic polymers where protein binding occurs predominantly via electrostatic…”

Development of Bioelectronic Devices Using Bionanohybrid Materials for Biocomputation System

“…As mentioned in the introduction, bioelectronic devices have certain limitations like the low electrical or electrochemical signal induced from biomolecules and low stability in harsh conditions [15,16]. To overcome these problems, various researchers have proposed the introduction of functional biocompatible nanomaterials for improved signal and stability [18,19,20].…”

“…Until now, many functional bioelectronic devices including protein-based bioelectronic chips that use the electron transfer mechanism of proteins and biophotodiode devices that use the photoelectric effect of rhodopsin have been reported [12,13,14]. However, current bioelectronic devices have certain critical limitations for practical application because the use of biomolecules inevitably accompanies limitations such as the low electrical/electrochemical signal-to-noise ratio derived from biomolecules, instability in harsh conditions, and narrow functionalization [15,16]. To overcome the limitations of biomolecules, innovative methods have been developed introducing nanoparticles to enhance the signal induced from biomolecules, combine biomolecules with carbon-based materials such as carbon nanotubes (CNT) or graphene for electrochemical signal increment and long-term stability using the biocompatibility of carbon-based materials, and the use of nanoscale-patterned chips as a platform for the extension of the functionality of bioelectronic devices such as by demonstrating nanoscale electronic functions and immobilizing different biomolecules independently at the nanometer scale to use these biomolecules simultaneously [17,18,19,20,21,22].…”

Electrochemical H2O2 biosensor composed of myoglobin on MoS2 nanoparticle-graphene oxide hybrid structure

“…However, the interaction between the metalloprotein and target molecule on a conventional electrode is met with limitations such as the unintended orientation of biomolecules on the electrode, low electrochemical signal derived from biomolecules, and slow, varying electron-transfer rate (Thudi et al, 2012;Kim et al, 2010). To overcome these, materials such as gold nanoparticles and new types of electrodes such as those based on dendritic polymers have been used to develop bioelectronic devices and biosensors with amplified electrochemical signal and sensitivity (Li et al, 2014a(Li et al, , 2014bJiménez et al, 2014;Cai et al, 2003).…”