Adsorption of Bovine Serum Albumin on Carbon-Based Materials

Seredych, Mykola; Mikhalovska, Lyuba; Mikhalovsky, Sergey V.; Gogotsi, Yury

doi:10.3390/c4010003

Cited by 35 publications

(32 citation statements)

References 41 publications

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“…This pseudo second order kinetic profile is consistent with the BSA absorption studies of others who have used other classes of particles, including graphene materials and -carrageenan/ carboxymethyl chitosan-coated materials. 27,44 These results suggest that BSA-BSA interactions on the nanoparticle surface are important for the adsorption interactions. This multi-layer formation is typical for protein adsorption onto a surface; at lower concentrations of protein, a monolayer forms, but as concentration increases, a multi-layer forms and entropically driven adsorption can be facilitated by the presence of the previous layer.…”

Section: Langmuir Isothermmentioning

confidence: 85%

Adsorption of bovine serum albumin (BSA) by bare magnetite nanoparticles with surface oxidative impurities that prevent aggregation

Rahdar

Ahmadi

et al. 2019

Can. J. Chem.

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Bare, uncoated magnetite nanoparticles, synthesized using an electrochemical surfactant-free synthesis, have highly oxidized surfaces that prevent aggregation. These particles have demonstrated highly intriguing biological activity showing extremely potent antibiotic activity against both gram-positive and gram-negative bacteria with little toxicity to rats. This difference in activity could be ascribed to the nature of the protein corona. The kinetics and thermodynamics of the binding of bovine serum albumin (BSA), used as a model serum protein, to these magnetite nanoparticles were analyzed. There is no significant change in particle diameter by dynamic light scattering following adsorption, indicating corona formation does not induce aggregation. The maximum adsorption capacity of the particles was determined to be 300 mg of BSA per gram of magnetite. The particles are able to adsorb 90% of the BSA at protein concentrations as high as 500 mg/L. The adsorption is best described using a pseudo second order model and a Langmuir Type III isotherm model. Thermodynamic analysis showed that the process is entropically driven and is spontaneous at all tested temperatures and conditions. However, it appears to be a weak to moderate physical adsorption. This moderate binding affinity could indicate the differential biological activity of these particles towards bacteria and mammalian cells and further support the contention that these are potentially useful new tools for targeting antibiotic-resistant bacteria.

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Section: Langmuir Isothermmentioning

confidence: 85%

Adsorption of bovine serum albumin (BSA) by bare magnetite nanoparticles with surface oxidative impurities that prevent aggregation

Rahdar

Ahmadi

et al. 2019

Can. J. Chem.

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“…The overall protein charge is weakened and electrostatic repulsion forces between the adsorbent and protein take place [65]. Moreover, the hydrophobic nature of the activated carbons result in effective adsorption of hydrophobic segments of the protein [66].…”

Section: Supplementary Materialsmentioning

confidence: 99%

Preparation of Activated Carbons from Spent Coffee Grounds and Coffee Parchment and Assessment of Their Adsorbent Efficiency

et al. 2021

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The valorization of coffee wastes through modification to activated carbon has been considered as a low-cost adsorbent with prospective to compete with commercial carbons. So far, very few studies have referred to the valorization of coffee parchment into activated carbon. Moreover, low-cost and efficient activation methods need to be more investigated. The aim of this work was to prepare activated carbon from spent coffee grounds and parchment, and to assess their adsorption performance. The co-calcination processing with calcium carbonate was used to prepare the activated carbons, and their adsorption capacity for organic acids, phenolic compounds and proteins was evaluated. Both spent coffee grounds and parchment showed yields after the calcination and washing treatments of around 9.0%. The adsorption of lactic acid was found to be optimal at pH 2. The maximum adsorption capacity of lactic acid with standard commercial granular activated carbon was 73.78 mg/g, while the values of 32.33 and 14.73 mg/g were registered for the parchment and spent coffee grounds activated carbons, respectively. The Langmuir isotherm showed that lactic acid was adsorbed as a monolayer and distributed homogeneously on the surface. Around 50% of total phenols and protein content from coffee wastewater were adsorbed after treatment with the prepared activated carbons, while 44, 43, and up to 84% of hydrophobic compounds were removed using parchment, spent coffee grounds and commercial activated carbon, respectively; the adsorption efficiencies of hydrophilic compounds ranged between 13 and 48%. Finally, these results illustrate the potential valorization of coffee by-products parchment and spent coffee grounds into activated carbon and their use as low-cost adsorbent for the removal of organic compounds from aqueous solutions.

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“…12 Although the phenomenon of biocompatibility is complex and its mechanisms not fully known, for those materials exposed to the human body, it is associated with the adsorption of proteins on their surface. 13 While working towards bioinspired surfaces, our study aims at the preparation of biocompatible graphene-based microchips, to induce graphene-cell interactions, as potential implantable devices. Here, we describe the selective functionalization of laser-induced GO patterns on graphene surfaces by protein adsorption via non-covalent interactions.…”

Section: Introductionmentioning

confidence: 99%