Adsorption of cationic polyacrylamide at the cellulose–liquid interface: A neutron reflectometry study

Su, Jielong; Garvey, Christopher J.; Holt, Stephen A.; Tabor, Richard Francis; Winther‐Jensen, Bjorn; Batchelor, Warren; Garnier, Gil

doi:10.1016/j.jcis.2015.02.008

Cited by 17 publications

(19 citation statements)

References 55 publications

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“…Specular neutron reflection (NR) is a very useful technique for direct investigation of phenomena at the solid/water and solid/air interfaces53131. The technique can also be employed to monitor the interfacial interactions between cellulose and bio-macromolecules under controlled conditions.…”

Section: Preparation Of DC Films From Dbcmentioning

confidence: 99%

“…Firstly, paper and wood both have complex three-dimensional hierarchical structures6 and their variable characteristics such as porosity, thickness, and chemical composition render the analysis of adsorption/desorption of antibodies and enzymes extremely difficult. Secondly, the overall chemical compositions and electron/neutron densities of cellulose and most bio-macromolecules are sufficiently close to limit opportunities for the contrast between a cellulose surface and an adsorbed biomolecule when examined by X-ray/neutron reflectometry7, spectroscopy and microscopy. These limitations have led various workers to use model cellulose films as a platform to investigate interfacial characteristics8910111213.…”

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confidence: 99%

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Smooth deuterated cellulose films for the visualisation of adsorbed bio-macromolecules

Raghuwanshi

Raverty

et al. 2016

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Novel thin and smooth deuterated cellulose films were synthesised to visualize adsorbed bio-macromolecules using contrast variation neutron reflectivity (NR) measurements. Incorporation of varying degrees of deuteration into cellulose was achieved by growing Gluconacetobacter xylinus in deuterated glycerol as carbon source dissolved in growth media containing D2O. The derivative of deuterated cellulose was prepared by trimethylsilylation(TMS) in ionic liquid(1-butyl-3-methylimidazolium chloride). The TMS derivative was dissolved in toluene for thin film preparation by spin-coating. The resulting film was regenerated into deuterated cellulose by exposure to acidic vapour. A common enzyme, horseradish peroxidase (HRP), was adsorbed from solution onto the deuterated cellulose films and visualized by NR. The scattering length density contrast of the deuterated cellulose enabled accurate visualization and quantification of the adsorbed HRP, which would have been impossible to achieve with non-deuterated cellulose. The procedure described enables preparing deuterated cellulose films that allows differentiation of cellulose and non-deuterated bio-macromolecules using NR.

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Section: Preparation Of DC Films From Dbcmentioning

confidence: 99%

mentioning

confidence: 99%

Smooth deuterated cellulose films for the visualisation of adsorbed bio-macromolecules

Raghuwanshi

Raverty

et al. 2016

Sci Rep

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“…The CPAM of charge density 10% and 50% (13 MDa) form a monolayer on microfibrillated cellulose (MFC) at 8 and 5 mg/g, respectively [24]; Charge neutrality (zeta potential, = 0), were achieved at the same CPAM/MFC ratios (MFC = −26 mV) [24]. MFC has a surface area of 35 m 2 /g versus 1 m 2 /g for most papers; the maximum CPAM surface coverage is 0.22 mg/m 2 and 0.14 mg/m 2 for the CPAM of 10% and 50% charge density, respectively [24].…”

Section: Effect Of Cpam On Rbc Retention and Diagnostic Specificitymentioning

confidence: 99%

“…MFC has a surface area of 35 m 2 /g versus 1 m 2 /g for most papers; the maximum CPAM surface coverage is 0.22 mg/m 2 and 0.14 mg/m 2 for the CPAM of 10% and 50% charge density, respectively [24]. With 100 L CPAM solutions droplets forming circles of diameter of 6 cm giving an area of 28 cm 2 and considering the above CPAM full surface coverages, it can be calculated with the paper towel (26.4 g/m 2 ), that full CPAM monolayer coverage are achieved at concentration of 0.16 g/L and 0.10 g/L for the 10% and 50% charge density CPAM.…”

Section: Effect Of Cpam On Rbc Retention and Diagnostic Specificitymentioning

confidence: 99%

Effect of cationic polyelectrolytes on the performance of paper diagnostics for blood typing

McLiesh

Sharman

Garnier

2015

Colloids and Surfaces B: Biointerfaces

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“…Thus, with most analytical techniques, antibodies become insignificant from the paper or pulp fibers onto which they are adsorbed. Using smooth cellulosic films as model substrates is a way to alleviate the effect of paper morphology from the study (Su et al, 2015). Therefore, the variables in antibody functionality loss on nanoscale cellulosic films can be limited to chemical property and interactions.…”

Section: Introductionmentioning

confidence: 99%

Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films

Huang

Raghuwanshi

Garnier

2017

Front. Bioeng. Biotechnol.

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The functionality and aging mechanism of antibodies physisorbed onto cellulosic films was investigated. Blood grouping antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) were adsorbed onto smooth cellulose acetate (CAF) and regenerated cellulose (RCF) films. Cellulose films and adsorbed IgG layers were characterized at the air and liquid interface by X-ray and neutron reflectivity (NR), respectively. Cellulose film 208 Å thick (in air) swell to 386 Å once equilibrated in water. IgG adsorbs from solution onto cellulose as a partial layer 62 Å thick. IgG and IgM antibodies were adsorbed onto cellulose and cellulose acetate films, air dried, and aged at room temperature for periods up to 20 days. Antibody functionality and surface hydrophobicity were measured everyday with the size of red blood cell (RBC) agglutinates (using RBC specific to IgG/IgM) and the water droplet contact angle, respectively. The functionality of the aged IgG/IgM decreases faster if physisorbed on cellulose than on cellulose acetate and correlates to surface hydrophobicity. IgG physisorbed on RCF or CAF age better and remain functional longer than physisorbed IgM. We found a correlation between antibody stability and hydrogen bond formation ability of the system, evaluated from antibody carbonyl concentration and cellulosic surface hydroxyl concentration. Antibody physisorbs on cellulose by weak dipole forces and hydrogen bonds. Strong hydrogen bonding contributes to the physisorption of antibody on cellulose into a non-functional configuration in which the molecule relaxes by rotation of hydophobic groups toward the air interface.

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Adsorption of cationic polyacrylamide at the cellulose–liquid interface: A neutron reflectometry study

Cited by 17 publications

References 55 publications

Smooth deuterated cellulose films for the visualisation of adsorbed bio-macromolecules

Smooth deuterated cellulose films for the visualisation of adsorbed bio-macromolecules

Effect of cationic polyelectrolytes on the performance of paper diagnostics for blood typing

Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films

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