Adult Celiac disease; clinical presentations and biochemical profiles in Hyderabad

Siddiqui, Naila Masood; Shaikh, Imran

doi:10.12669/pjms.302.3844

Cited by 5 publications

(6 citation statements)

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“…Phenylmethanesulfonyl fluoride (PMSF) was used as a reference inhibitor. All experiments were performed in triplicate [19–20] . The IC 50 values were calculated using GraphPad Prism 6 software.…”

Section: Methodsmentioning

confidence: 99%

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Chymotrypsin and Trypsin Inhibitory Activity of Some Medicinal Plants Collected from Rize (Türkiye)

Gunbatan,

Sucu,

Gokbulut

et al. 2024

Chemistry & Biodiversity

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In this research, the evaluation of in vitro chymotrypsin and trypsin inhibitory activities of ten plant species collected from Rize were aimed, and fractions that showed strong activity were analyzed through HPLC. Daphne pontica L. and Mentha longifolia (L.) L. were found to have the highest chymotrypsin inhibitory activities (87.75 and 84.24% inhibition). Similarly, the highest trypsin inhibitory activity was observed in D. pontica (%99.93 inhibition), followed by Sambucus ebulus L. flowers (87.47% inhibition). Extracts showing strong enzyme inhibition were fractioned and subjected to activity tests. The highest chymotrypsin inhibitory activity was observed in the n‐hexane fraction of D. pontica (%80.70 inhibition), while the highest trypsin inhibitory activity was found in the n‐butanol fraction of S. ebulus (%86.81 inhibition). HPLC studies determined that the 80% ethanol extract of D. pontica and its dichloromethane and ethyl acetate fractions contained umbelliferone. It was found that chlorogenic acid was present in the 80% ethanol extracts of S. ebulus flowers. M. longifolia was found to contain chlorogenic acid, caffeic acid, luteolin‐7‐glucoside, and rosmarinic acid. M. longifolia has been identified as the plant exhibiting the highest antioxidant activity in ABTS and CUPRAC tests, consistent with its high phenolic and flavonoid content.

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Section: Methodsmentioning

confidence: 99%

“…All experiments were performed in triplicate. [19][20] The IC 50 values were calculated using GraphPad Prism 6 software. The percentage of inhibition was calculated using the following formula:…”

Section: Determination Of Chymotrypsin Inhibitory Activitymentioning

confidence: 99%

Chymotrypsin and Trypsin Inhibitory Activity of Some Medicinal Plants Collected from Rize (Türkiye)

Gunbatan,

Sucu,

Gokbulut

et al. 2024

Chemistry & Biodiversity

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“…It is possible to utilize it to guide a brush biopsy without interfering with the computerassisted cytological analysis of these tissues (10). It was reported that for many years, tolonium chloride has been successfully used as a key stain and a tool in the early diagnosis of oral cancer, cancer of the oral mucosa, and cancer of the upper aerodigestive tract (11). It is advised to use TC + in addition to chemiluminescence or other methods as a diagnostic tool (12).…”

Section: Introductionmentioning

confidence: 99%

Degradation of Tolonium Chloride Dye by Phosphate Ion in Aqueous Acidic Solution: Kinetic Approach

Umoru,

Lawal,

Babatunde

et al. 2024

Journal of the Turkish Chemical Society Section A: Chemistry

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The degradation of tolonium chloride (TC+) dye by phosphate ion (PO43-) in an aqueous acidic solution was studied using spectrophotometric analysis at 301 K, I= 1.0 M, [TC+]= 1.5 × 10-5 M, [H+]= 1.0×10-3 M, and ʎmax 600 nm. To determine the potency and rate of the reactant species, an aqueous acidic medium was employed. The reaction's direction and tendency were predicted using a thermodynamic analysis at an interval of 5.0 K and a temperature range of 301-321 K. Without the presence of intermediate complex/free atoms formation, a reaction that produced phenyl sulphoxide, phenylamine, and HPO32- as products of the reaction was obtained with a molar ratio of 1:1 for both reactants. First-order tolonium chloride reactivity was found in the reaction and first-order for the phosphate ion, resulting in a second-order reaction overall. The reaction process accelerated as the concentration of hydrochloric acid rose. The response time decreased with an increase in ionic strength concentration and added Ca2+ and Cl- did catalyze the reaction positively. A straight line that went through the origin was produced by plotting 1/ko vs PO43- concentration. The spectroscopic analysis showed no discernible shift from λmax of 600 nm. Additionally, an increase in temperature accelerated the reaction process. The reaction has a negative free energy change, G (-3.13–1.12 KJ/mol) which indicates that it is spontaneous and that the reactants have more free energy than that of the products. While the enthalpy of activation, H is positive and indicates that the reaction was endothermic and followed an associative path, the entropy of activation, S, is also negative (-7.45–1.10 KJ/mol), indicating that the reaction is less disordered. Due to the added ions catalysis and absence of free atoms during the course of the reaction, an outer-sphere mechanism was suggested for the reaction.

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“…Worldwide, oral cancer accounts for 2%-4% of all cancer cases. In a number of regions, the frequency of oral cancer is higher, reaching the 10% of all cancers in Pakistan, and around 45% in India [1][2]. In 2004-2009 over 300,000 new cases of oral and oropharyngeal cancer were diagnosed worldwide.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Study of Antioxidant-Oxidant Index in Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

Shukla

2021

JASR

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The aim of present work was to evaluate and compare the salivary levels of nitric oxide, vitamin C, Total Sialic acid and GSH in cases of Oral Potentially Malignant Disorders (OPMDs) and Oral Squamous Cell Carcinoma (OSCC) and healthy controls. The mean nitric oxide level was 27.34±5.51 μmol/l in OSCC group, 22.5±2.33 μmol/l in OPMD group and 10.11±0.88μmol/l in control group. The glutathione reductase activity in control patients was found to be 0.0915 U/ml under optimal pH, temperature and Km. In OPMD group, the GR activity was found to be 0.0515 U/ml. Similarly, the activity in the OSCC group was found to be 0.0292 U/ml. The total sialic acid (TSA) in the saliva of control patients was found to be 41.241±5.3312μg/mL. In the case of OPMD patients, it was 64.25±4.33μg/mL and in the OSCC patients it was, 79.60±6.93 μg/mL., The levels of salivary vitamin C and glutathione were significantly reduced and those of nitric oxide and sialic acid were raised in patients having OPMD’s and oral squamous cell carcinoma. The anti-oxidant to oxidant index (AOI) was measured between NO and vitamin C; NO and GSH; total sialic acid and vitamin C and lastly, total sialic acid and GSH. Thus, the findings of the present study indicate that estimation of Vitamin C, NO, GSH and sialic acid can be suitably used and could assist in the early diagnosis of potentially malignant disorders and oral cancer using saliva.

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Adult Celiac disease; clinical presentations and biochemical profiles in Hyderabad

Cited by 5 publications

References 0 publications

Chymotrypsin and Trypsin Inhibitory Activity of Some Medicinal Plants Collected from Rize (Türkiye)

Chymotrypsin and Trypsin Inhibitory Activity of Some Medicinal Plants Collected from Rize (Türkiye)

Degradation of Tolonium Chloride Dye by Phosphate Ion in Aqueous Acidic Solution: Kinetic Approach

Biochemical Study of Antioxidant-Oxidant Index in Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

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