Advanced in vitro models of vascular biology: Human induced pluripotent stem cells and organ-on-chip technology

Cochrane, Amy; Albers, Hugo J.; Passier, Robert; Mummery, Christine L.; Berg, Albert van den; Orlova, Valeria V.; Meer, Andries D. van der

doi:10.1016/j.addr.2018.06.007

Cited by 127 publications

(89 citation statements)

References 119 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 515 ] Human induced pluripotent stem cells (hiPSCs) could potentially provide an option, having been directed toward EC phenotype; efforts are ongoing, however, to obtain more mature and functional organ‐specific behavior and gene expression. [ 516 ] The use of patient‐derived cells for individualized medicine has also gained traction as a method to more accurately recapitulate in vivo conditions of ECs in in vitro devices; a recent study cultured patient‐specific renal cell carcinoma tumor‐derived and normal kidney ECs in in vitro vascular models to screen antiangiogenic therapies on an individualized basis. [ 517 ]…”

Section: Current Limitations and Future Opportunitiesmentioning

confidence: 99%

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Pradhan

Banda

Farino

et al. 2020

Adv Healthcare Materials

View full text Add to dashboard Cite

The vascular system is integral for maintaining organ‐specific functions and homeostasis. Dysregulation in vascular architecture and function can lead to various chronic or acute disorders. Investigation of the role of the vascular system in health and disease has been accelerated through the development of tissue‐engineered constructs and microphysiological on‐chip platforms. These in vitro systems permit studies of biochemical regulation of vascular networks and parenchymal tissue and provide mechanistic insights into the biophysical and hemodynamic forces acting in organ‐specific niches. Detailed understanding of these forces and the mechanotransductory pathways involved is necessary to develop preventative and therapeutic strategies targeting the vascular system. This review describes vascular structure and function, the role of hemodynamic forces in maintaining vascular homeostasis, and measurement approaches for cell and tissue level mechanical properties influencing vascular phenomena. State‐of‐the‐art techniques for fabricating in vitro microvascular systems, with varying degrees of biological and engineering complexity, are summarized. Finally, the role of vascular mechanobiology in organ‐specific niches and pathophysiological states, and efforts to recapitulate these events using in vitro microphysiological systems, are explored. It is hoped that this review will help readers appreciate the important, but understudied, role of vascular‐parenchymal mechanotransduction in health and disease toward developing mechanotherapeutics for treatment strategies.

show abstract

Section: Current Limitations and Future Opportunitiesmentioning

confidence: 99%

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Pradhan

Banda

Farino

et al. 2020

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…As an example, Ciampi et al constructed a vascularized kidney organoid by engineering kidney vasculatures using human iPSC‐derived ECs . In general, the current protocol for deriving these cells of iPSC origin begins with providing the right combination of growth factors to induce differentiation of iPSCs toward the mesoderm fate . Then, EC specification is induced by the addition of growth factor such as VEGF .…”

Section: Design Consideration: How Simple Is Complex Enough?mentioning

confidence: 99%

“…In general, the current protocol for deriving these cells of iPSC origin begins with providing the right combination of growth factors to induce differentiation of iPSCs toward the mesoderm fate . Then, EC specification is induced by the addition of growth factor such as VEGF . However, due to the lack of standardized and reproducible protocols, typically only personnel with extensive stem cell culture experience are able to successfully differentiate them .…”

Section: Design Consideration: How Simple Is Complex Enough?mentioning

confidence: 99%

Recreating Physiological Environments In Vitro: Design Rules for Microfluidic‐Based Vascularized Tissue Constructs

Tan

Leung

2020

Small

View full text Add to dashboard Cite

Vascularization of engineered tissue constructs remains one of the greatest unmet challenges to mimicking the native tissue microenvironment in vitro. The main obstacle is recapitulating the complexity of the physiological environment while providing simplicity in operation and manipulation of the model. Microfluidic technology has emerged as a promising tool that enables perfusion of the tissue constructs through engineered vasculatures and precise control of the vascular microenvironment cues in vitro. The tunable microenvironment includes i) biochemical cues such as coculture, supporting matrix, and growth factors and ii) engineering aspects such as vasculature engineering methods, fluid flow, and shear stress. In this systematic review, the design considerations of the microfluidic‐based in vitro model are discussed, with an emphasis on microenvironment control to enhance the development of next‐generation vascularized engineered tissues.

show abstract

“…Until recently, primary and immortalized cells from both animals and humans, respectively, were used to develop brain angiogenesis models. The recent development of the gene editing technology, such as CRISPR/Cas9, led to the use of Human induced pluripotent stem cells (HiPSC) derived from both healthy and diseased humans [93]. Although it is very challenging to maintain and standardize HiPSC protocols, microfluidics platforms once again have aided in sustaining more complex, dynamic and controlled environment for using HiPSC [2].…”

Section: Establishing Brain Vasculature On Chipsmentioning

confidence: 99%

Lab-On-A-Chip for the Development of Pro-/Anti-Angiogenic Nanomedicines to Treat Brain Diseases

Parimalam

Bădilescu

Sonenberg

et al. 2019

IJMS

View full text Add to dashboard Cite

There is a huge demand for pro-/anti-angiogenic nanomedicines to treat conditions such as ischemic strokes, brain tumors, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Nanomedicines are therapeutic particles in the size range of 10–1000 nm, where the drug is encapsulated into nano-capsules or adsorbed onto nano-scaffolds. They have good blood–brain barrier permeability, stability and shelf life, and able to rapidly target different sites in the brain. However, the relationship between the nanomedicines’ physical and chemical properties and its ability to travel across the brain remains incompletely understood. The main challenge is the lack of a reliable drug testing model for brain angiogenesis. Recently, microfluidic platforms (known as “lab-on-a-chip” or LOCs) have been developed to mimic the brain micro-vasculature related events, such as vasculogenesis, angiogenesis, inflammation, etc. The LOCs are able to closely replicate the dynamic conditions of the human brain and could be reliable platforms for drug screening applications. There are still many technical difficulties in establishing uniform and reproducible conditions, mainly due to the extreme complexity of the human brain. In this paper, we review the prospective of LOCs in the development of nanomedicines for brain angiogenesis–related conditions.

show abstract

Advanced in vitro models of vascular biology: Human induced pluripotent stem cells and organ-on-chip technology

Cited by 127 publications

References 119 publications

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Recreating Physiological Environments In Vitro: Design Rules for Microfluidic‐Based Vascularized Tissue Constructs

Lab-On-A-Chip for the Development of Pro-/Anti-Angiogenic Nanomedicines to Treat Brain Diseases

Contact Info

Product

Resources

About