Advanced therapies of skin injuries

Maver, Tina; Maver, Uroš; Stana‐Kleinschek, Karin; Mlinarič-Raščan, Irena; Smrke, Dragica

doi:10.1007/s00508-015-0859-7

Cited by 39 publications

(27 citation statements)

References 61 publications

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“…The development of novel solutions related to any tissue engineering application consumes a huge number of cells to prove safety and efficiency (Groeber et al, 2012; Maver et al, 2015; Mohd Hilmi & Halim, 2015; Rodriguez-Vazquez et al, 2015). Cartilage tissue engineering is no exception, and hence large scale expansion of chondrocytes is required either for novel scaffold testing, determination of potential cytotoxic effects of medical devices and implants for orthopaedic use (Bomer et al, 2016; Camarero-Espinosa et al, 2016; Makris et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA)

Naranda

Gradišnik

Gorenjak

et al. 2017

PeerJ

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BackgroundCartilage tissue engineering is a fast-evolving field of biomedical engineering, in which the chondrocytes represent the most commonly used cell type. Since research in tissue engineering always consumes a lot of cells, simple and cheap isolation methods could form a powerful basis to boost such studies and enable their faster progress to the clinics. Isolated chondrocytes can be used for autologous chondrocyte implantation in cartilage repair, and are the base for valuable models to investigate cartilage phenotype preservation, as well as enable studies of molecular features, nature and scales of cellular responses to alterations in the cartilage tissue.MethodsIsolation and consequent cultivation of primary human adult articular chondrocytes from the surgical waste obtained during total knee arthroplasty (TKA) was performed. To evaluate the chondrogenic potential of the isolated cells, gene expression of collagen type 2 (COL2), collagen 1 (COL1) and aggrecan (ACAN) was evaluated. Immunocytochemical staining of all mentioned proteins was performed to evaluate chondrocyte specific production.ResultsCartilage specific gene expression of COL2 and ACAN has been shown that the proposed protocol leads to isolation of cells with a high chondrogenic potential, possibly even specific phenotype preservation up to the second passage. COL1 expression has confirmed the tendency of the isolated cells dedifferentiation into a fibroblast-like phenotype already in the second passage, which confirms previous findings that higher passages should be used with care in cartilage tissue engineering. To evaluate the effectiveness of our approach, immunocytochemical staining of the evaluated chondrocyte specific products was performed as well.DiscussionIn this study, we developed a protocol for isolation and consequent cultivation of primary human adult articular chondrocytes with the desired phenotype from the surgical waste obtained during TKA. TKA is a common and very frequently performed orthopaedic surgery during which both femoral condyles are removed. The latter present the ideal source for a simple and relatively cheap isolation of chondrocytes as was confirmed in our study.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA)

Naranda

Gradišnik

Gorenjak

et al. 2017

PeerJ

Self Cite

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“…The inspiration for regenerative medicine strategies is commonly derived from the increased understanding of basic wound healing processes; (Briquez, Hubbell, & Martino, ; Maver, Maver, Kleinschek, Raščan, & Smrke, ) such strategies aim to replicate and reinforce biological activities that occur during embryonic development (Maver et al, ; Yu, Khalil, Dang, Alsberg, & Murphy, ). As such, in an effort to satisfy the comprehensive demands of skin regeneration, the biomimetic delivery of key growth factors could potentially promote wound healing (Kim & Tabata, ).…”

Section: Introductionmentioning

confidence: 99%

The dual delivery of KGF and bFGF by collagen membrane to promote skin wound healing

Cao

et al. 2018

J Tissue Eng Regen Med

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The major challenges associated with skin regeneration can include hindered vascularization and an insufficient degree of epithelization. In view of the complexity of these processes and the control signals on which they depend, one possible solution to these limitations could be simulating normal skin development and wound repair via the exogenous delivery of multiple cytokines. Here, we report the use of keratinocyte growth factor (KGF or FGF-7) and basic fibroblast growth factor (bFGF or FGF-2) released chemically modified collagen membranes to facilitate skin wound healing. The results from in vitro studies confirmed that this system resulted in higher cellular proliferation and faster cell migration. After transplanting the biomaterial onto an excisional wound healing model, the dual growth factor group, compared with the single growth factor groups and empty control group, showed more highly developed vascular networks and organized epidermal regeneration in the wounds. As a consequence, this experimental group showed mature epidermal coverage. Overall, this novel approach of releasing growth factors from a collagen membrane opens new avenues for fulfilling unmet clinical needs for wound care.

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“…Because various scaffold materials and cell sources are used in tissue engineering, advances in materials science are particularly important for the development of this field. These include improvements in manufacturing techniques, material processing and development, surface functionalization, drug delivery systems and cell incorporation [16][17][18][19].…”

Section: Intervertebral Disc Structurementioning

confidence: 99%

Intervertebral disc tissue engineering: A brief review

Stergar

Gradišnik

Velnar

et al. 2019

Bosn J of Basic Med Sci

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Intervertebral disc (IVD) degeneration (IDD) is associated with low back pain and significantly affects the patient’s quality of life. Degeneration of the IVD alters disk height and the mechanics of the spine, leading to chronic segmental spinal instability. The pathophysiology of IVD disease is still not well understood. Current therapies for IDD include conservative and invasive approaches, but none of those treatments are able to restore the disc structure and function. Recently, tissue engineering techniques emerged as a possible approach to treat IDD, by replacing a damaged IVD with scaffolds and appropriate cells. Advances in manufacturing techniques, material processing and development, surface functionalization, drug delivery systems and cell incorporation furthered the development of tissue engineering therapies. In this review, biomaterial scaffolds and cell-based therapies for IVD regeneration are briefly discussed.

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Advanced therapies of skin injuries

Cited by 39 publications

References 61 publications

Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA)

Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA)

The dual delivery of KGF and bFGF by collagen membrane to promote skin wound healing

Intervertebral disc tissue engineering: A brief review

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