Advancements in the gold standard: Measuring steroid sex hormones by mass spectrometry

Conklin, Steven E.; Knezevic, Claire E.

doi:10.1016/j.clinbiochem.2020.03.008

Cited by 41 publications

(31 citation statements)

References 149 publications

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“…Analytica Chimica Acta, 1,089, 100-107. https://doi.org/10.1016/j. aca.2019.09.058 endocrinology community (Conklin & Knezevic, 2020;Rosner et al, 2007). This technique allows for simultaneous quantification of testosterone, estradiol and progesterone, as well as other steroid hormones and metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…During the method development, this assay was applied to quantifying testosterone from male and female donors, achieving a limit of F I G U R E 2 Structures of progesterone, testosterone, and estradiol. reprinted ( Figure 1) with permission from Conklin, S. E., & Knezevic, C. E. (2020). Advancements in the gold standard: Measuring steroid sex hormones by mass spectrometry.…”

Section: Testosterone Bioanalysismentioning

confidence: 99%

“…Advancements in MS development have followed an exciting timeline which has led to the widely used current practice of LC–MS for the measurement of hormones (Shackleton, 2010). Mass spectrometry has transitioned over the past 20 years toward the status of “gold standard” for hormone analysis (Conklin & Knezevic, 2020; Wudy & Choi, 2016). Setting the stage for the practical use of MS studies in hormone measurement was the gas‐chromatographic (GC) separation of sterols in the 1960s (Eneroth, Hellstroem, & Ryhage, 1964).…”

Section: Introduction To Sex Steroid Hormone Lc–msmentioning

confidence: 99%

“…The purpose of this review was to summarize some of the trends in sex steroid hormone quantification over the past 10 years, including testosterone, progesterone and 17‐ β estradiol. The structures of these hormones can be seen in Figure 2 (Conklin & Knezevic, 2020). Despite a history of mass spectrometric analysis that begins in the 1960s, steroid bioanalysis is and remains a challenging undertaking.…”

Section: Introduction To Sex Steroid Hormone Lc–msmentioning

confidence: 99%

“…Structures of progesterone, testosterone, and estradiol. reprinted (Figure 1) with permission from Conklin, S. E., & Knezevic, C. E. (2020). Advancements in the gold standard: Measuring steroid sex hormones by mass spectrometry.…”

Section: Introduction To Sex Steroid Hormone Lc–msmentioning

confidence: 99%

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Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

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Liquid chromatography, coupled with tandem mass spectrometry, presents a powerful tool for the quantification of the sex steroid hormones 17-β estradiol, progesterone and testosterone from biological matrices. The importance of accurate quantification with these hormones, even at endogenous levels, has evolved with our understanding of the role these regulators play in human development, fertility and disease risk and manifestation. Routine monitoring of these analytes can be accomplished by immunoassay techniques, which face limitations on specificity and sensitivity, or using gas chromatography-mass spectrometry. LC-MS/MS is growing in capability and acceptance for clinically relevant quantification of sex steroid hormones in biological matrices and is able to overcome many of the limitations of immunoassays. Analyte specificity has improved through the use of novel derivatizing agents, and sensitivity has been refined through the use of high-resolution chromatography and mass spectrometric technology. This review highlights these innovations, among others, in LC-MS/MS steroid hormone analysis captured in the literature over the last decade.

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Section: Discussionmentioning

confidence: 99%

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Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

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Liquid chromatography–tandem mass spectrometry measurement of 26 steroid hormones in human serum and plasma samples

Jia¹,

Liu²,

Xu³

et al. 2021

J of Separation Science

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The measurement of steroid hormones provided critical information in the clinical evaluation of endocrine disorders. In this study, we developed a highthroughput solid-phase extraction method for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography-tandem mass spectrometry. Chromatographic conditions and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes.Under the optimum conditions, good linearities were achieved in the quantitative range for each steroid hormone with the correlation coefficients (r) larger than 0.99. The limits of quantitation of the method were in the range from 0.0005 to 0.7901 ng/mL. The recoveries were in the range of 87.2-114.2% with intra-and interday precision lower than 9.94%. This method has already been applied to series of samples from clinical trials, and there was no significant difference between serum and ethylenediaminetetraacetic acid plasma samples.

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A Dual‐Modal Single‐Antibody Plasmonic Spectro‐Immunoassay for Detection of Small Molecules

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Small molecules play a pivotal role in regulating physiological processes and serve as biomarkers to uncover pathological conditions and the effects of therapeutic treatments. However, it remains a significant challenge to detect small molecules given the size as compared to macromolecules. Recently, the newly emerging plasmonic immunoassays based on surface‐enhanced Raman scattering (SERS) offer great promise to deliver extraordinary sensitivity. Nevertheless, they are limited by the intrinsic SERS intensity fluctuations associated with the SERS uncertainty principle. The single transducer that relies on the intensity change is also prone to false signals. Additionally, the prevailing sandwich immunoassay format proves less effective towards detecting small molecules. To circumvent these critical issues, a dual‐modal single‐antibody approach that synergizes both the intensity and shift of the peak‐based immunoassay with Raman enhancement, coined as the INSPIRE assay, is developed for small molecules detection. With two independent transduction mechanisms, it allows better prediction of analyte concentration and attenuation of signal artifacts, providing a new and robust strategy for molecular analysis. With a proof‐of‐concept demonstration for detection of free T4 and testosterone in serum matrix, the authors envision that the INSPIRE assay could be expanded for a wide spectrum of applications in biomedical diagnosis, discovery of new biopharmaceuticals, food safety, and environmental monitoring.

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Advancements in the gold standard: Measuring steroid sex hormones by mass spectrometry

Cited by 41 publications

References 149 publications

Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

Liquid chromatography–tandem mass spectrometry measurement of 26 steroid hormones in human serum and plasma samples

A Dual‐Modal Single‐Antibody Plasmonic Spectro‐Immunoassay for Detection of Small Molecules

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