Adventitious root culture for secondary metabolite production in medicinal plants: A Review

Abstract

Medicinal plants are high-value natural resources that have been used as precautionary drugs by many people globally. The increasing global demand for bioactive compounds from medicinal plants has led to the overexploitation of many valuable species. One widely used approach to overcome this problem is the use of adventitious root cultures as a propagation strategy. This review examines the scientific research published globally on the application of adventitious root cultures for many medicinal plants. Adventitious roots generated under aseptic environments in suitable phytohormone-augmented medium exhibit high growth rates and production of important secondary metabolites. Parameters such as medium properties and composition, growth hormone type, and elicitation strategies for in vitro grown adventitious roots of medicinal plants, are the main topics discussed in this review. We also examine current developments in bioreactor system cultivation for plant bioactive compounds using adventitious root cultures, a technology with possible commercial applications, via several studies on adventitious root culture of medicinal plants in which bioreactor systems play a role. In conclusion, the development of adventitious root cultures for medicinal plants is highly useful because of their capability for vegetative propagation and germplasm preservation.

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

“…A number of recent reviews deals with plant tissue culture for the production of secondary compounds, for example by Chandran et al ( 2020 ), Gutierrez-Valdes et al ( 2020 ), Marchev et al ( 2020 ), Cardoso et al ( 2019 ), Kreis ( 2019 ), Rahmat and Kang ( 2019 ), and more. The present mini-review on the one hand highlights recent achievements in the production of medically important plant secondary metabolites using various in vitro technologies, including most recent approaches like metabolic engineering and heterologous production in microorganisms.…”

Enhanced Aryltetralin Lignans Production in Linum Adventi-Tious Root Cultures

“…The results obtained indicate that ARc could be a very promising tissue for secondary metabolites production especially when using the proper elicitor. This technology could have commercial applications through a scale-up process in bioreactors as reported for some species such as Hypericum perforatum and Polygonum multiflorum [43]. The main advantage for the use of ARc with respect to hairy roots is the absence of opine-like substrates (dangerous to mammalian cells) that are usually produced as byproducts of the hairy root system [44].…”

Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends

“…In order to generate an adventitious root culture (Figure 1C), the callus must first be induced and a maintenance culture in Petri dishes established, as with the DDC‐based suspension culture [67]. Afterwards, root induction is carried out with a solid culture medium that supports root formation.…”