AEG-1 associates with metastasis in papillary thyroid cancer through upregulation of MMP2/9

Huang, Leilei; Wang, Zhuo; Cao, Chuang-Jie; Ke, Zunfu; Wang, Fen; Wang, Ran; Luo, Canqiao; Lu, Xiufen; Wang, Liantang

doi:10.3892/ijo.2017.4074

Cited by 51 publications

(44 citation statements)

References 31 publications

(35 reference statements)

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“…36 Studies have shown that the expression of MMP-2 and MMP-9 is positively correlated with lymph node metastases and degree of tumor invasion in PTC. 37,38 In order to further verify the correlation of UCA1 expression with cervical lymph node metastasis, IHC experiments were carried out to verify the correlation between UCA1 and lymph node metastases. We detected high levels of MMP-2 and MMP-9 in samples with high UCA1 expression.…”

Section: Discussionmentioning

confidence: 99%

<p>Correlations of lncRNAs with cervical lymph node metastasis and prognosis of papillary thyroid carcinoma</p>

Cui

et al. 2019

OTT

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Background Cervical lymph node metastasis is an important prognostic indicator for papillary thyroid carcinoma (PTC) and affects treatment strategies for PTC. lncRNAs essentially contribute to the biological functions of tumors. This study aimed to identify the lncRNAs associated with cervical lymph node metastasis and prognosis of PTC and their potential pathophysiological mechanisms. Materials and methods PTC-associated lncRNAs were selected from The Cancer Genome Atlas database, and correlations among lncRNAs, lymph node metastasis, tumor staging, and prognosis of PTC were analyzed in silico. These correlations were then validated through quantitative reverse transcription PCR (qRT-PCR) and immunohistochemistry (IHC). Results In silico analysis showed that FAM95B1 and UCA1 were significantly correlated with cervical lymph node metastasis, tumor staging, and PTC prognosis ( P <0.05). qRT-PCR analysis revealed high UCA1 expression in PTC tissues and correlations between UCA1 expression levels and cervical lymph node metastasis and tumor staging in PTC, that is, higher UCA1 expression resulted in poorer PTC prognosis. IHC analysis revealed that a high expression of UCA1 was accompanied by a high expression of metastasis-related proteins (MMP-2 and MMP-9), thereby validating the correlation of UCA1 expression with metastasis. Conclusion FAM95B1 and UCA1 expression was significantly correlated with the occurrence and progression of PTC. The expression levels of UCA1 significantly affected the prognosis of PTC patients and were significantly correlated with tumor staging and cervical lymph node metastasis.

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Section: Discussionmentioning

confidence: 99%

<p>Correlations of lncRNAs with cervical lymph node metastasis and prognosis of papillary thyroid carcinoma</p>

Cui

et al. 2019

OTT

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“…It has two outstanding features. The first is the high rate of metastasis to lymph nodes or other organs, which is as high as 30% to 59.1% . The second is the resistance to chemotherapy and conventional external beam radiotherapy .…”

Section: Introductionmentioning

confidence: 99%

“…Thyroid cancer is one of the fastest growing cancers worldwide. 1,2 It has two outstanding features. The first is the high rate of metastasis to lymph nodes or other organs, which is as high as 30% to 59.1%.…”

Section: Introductionmentioning

confidence: 99%

“…The first is the high rate of metastasis to lymph nodes or other organs, which is as high as 30% to 59.1%. 1,[3][4][5] The second is the resistance to chemotherapy and conventional external beam radiotherapy. 6,7 Metastatic thyroid cancer behaves aggressively and about two-thirds of patients with metastatic disease ultimately present with radioiodine refractory disease.…”

Section: Introductionmentioning

confidence: 99%

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Nevirapine inhibits migration and invasion in dedifferentiated thyroid cancer cells

et al. 2019

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BackgroundMetastatic or recurrent thyroid cancer often behaves aggressively, and approximately two‐thirds of patients present with radioiodine resistance. Effective therapies to suppress thyroid cancer metastasis are urgently needed. Nevirapine has been proved to suppress tumor growth and induce differentiation in several tumor cells, but has not previously been evaluated in metastasis of thyroid cancer. The present study aimed to investigate the effect of nevirapine on migration and invasion in dedifferentiated thyroid cancer cells.MethodsHuman dedifferentiated thyroid cancer cell line (WRO 82‐1) was subject to real‐time quantitative PCR, western blot and transwell migration/invasion assays. The liver metastasis in tumor xenografts of nude mice was subject to hematoxylin‐eosin (HE) staining.ResultsNevirapine significantly repressed cell migration and invasion in WRO 82‐1 cells, and surprisingly significantly decreased liver metastatic tumor in the nude mouse model of dedifferentiated thyroid cancer compared with that of the control. Moreover, nevirapine significantly decreased the expression of IL‐6 mRNA and phosphorylation of JAK2 (Y1007+Y1008) and STAT3 (Tyr 705) in WRO 82‐1 cells compared with those in control cells.ConclusionOur findings suggest that nevirapine significantly repressed migration and invasion/metastasis in WRO 82‐1 cells and tumor xenografts, which may be related to inhibition of IL‐6/STAT3 signaling pathway. It promises great potential as a novel therapy for thyroid cancer, especially for those patients with metastasis.

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“…Several studies have also found that PTC is associated with overexpression of MMP-1, MMP-2, MMP-7, MMP-9, and MMP-13. [11][12][13][14][15][16][17] However, few studies have reported on the correlation between PTC and other MMPs or the regulatory mechanisms of MMP expression in PTC. In the current study, we found that MMP-11 played an important role in the development of PTC.…”

Section: Introductionmentioning

confidence: 99%

MMP‐11 promotes papillary thyroid cell proliferation and invasion via the NF‐κB pathway

Zhang

Dong

et al. 2018

J of Cellular Biochemistry

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Papillary thyroid carcinoma (PTC) is the most common form of thyroid cancer, and its incidence is on the rise. It has been reported that some matrix metalloproteinases (MMPs) are abnormally expressed in PTC and can be used as diagnostic markers. However, few studies have explored the underlying mechanisms by which MMPs promote tumor progression. In this study, we used microarray analysis to compare the variations of gene expression within the PTC cell populations and their adjacent normal tissues and found that MMP-11 was the most differentially expressed MMP. To investigate the role of MMP-11 in the mediation of thyroid cancer cell development, pEnter-MMP-11 plasmid, and MMP-11 small interfering RNA were applied to up- and downregulate MMP-11 expression of in cultured PTC cell lines K1 and BCPAP. The results suggested that the levels of proliferation and migration of cells transfected with MMP-11 siRNA were significantly reduced, while the levels in MMP-11-plasmid-transfected cells were increased. In terms of the mechanism, experimental data showed that the change in cyclin D1 is consistent with MMP-11 expression, which may explain the changes in proliferation. In addition, Western blot assay was conducted to analyze the p65 and activated (phospho-) p65 protein levels concomitant with MMP-11 adjustments. Variations in intracellular MMP-11 significantly altered the amount of phospho-p65 in thyroid cells, while p65 knockdown did not affect MMP-11 expression. These results suggest that MMP-11 is located upstream of p65 and regulates its activity. Interestingly, the data for the Transwell assay suggested that MMP-11 regulatory migration is also associated with the NF-κB p65 signaling pathway. In conclusion, this report describes the important role of MMP-11 in the regulation of thyroid cell proliferation and migration. Mechanistic studies have shown that cyclin D1 and p65 are important mediators in the processes, which provides a new way to study the mechanism of MMPs promoting the progression of thyroid cancer.

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AEG-1 associates with metastasis in papillary thyroid cancer through upregulation of MMP2/9

Cited by 51 publications

References 31 publications

<p>Correlations of lncRNAs with cervical lymph node metastasis and prognosis of papillary thyroid carcinoma</p>

<p>Correlations of lncRNAs with cervical lymph node metastasis and prognosis of papillary thyroid carcinoma</p>

Nevirapine inhibits migration and invasion in dedifferentiated thyroid cancer cells

MMP‐11 promotes papillary thyroid cell proliferation and invasion via the NF‐κB pathway

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