Aeration requirements ofRhizobium cultures

Balatti, A P; Mazza, L.; Moretti, Érika Rabello

doi:10.1007/bf00933576

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…Mannitol is universally used for cultivation, both for fast-growing Rhizobium and slow-growing Bradyrhizobium, but for the latter arabinose and gluconate are much better carbon sources (Elkan and Kwik 1968). Surprisingly, glycerol, which can support a very high bacterial density (Balatti 1982;Balatti et al 1987), is not often used, although its metabolism has been studied thoroughly by Arias and Martinez-Drets (1976).…”

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confidence: 98%

Cultivation of Bradyrhizobium CB756 on sucrose prefermented by yeast

Lie¹,

Muilenburg²,

Hiep³

et al. 1992

Can. J. Microbiol.

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LIE, T. A., MUILENBURG, M., HIEP, N. H., and AYHAN, K. 1992. Cultivation of Bradyrhizobiurn CB756 on sucrose prefermented by yeast. Can. J. Microbiol. 38: 569-572. Saccharornyces cerevisiae, grown with sucrose as a substrate, converts the sucrose to glycerol and produces yeast cells. The yield of glycerol was doubled by adding CaCO,. The fermented medium, containing glycerol and yeast cells, was an excellent medium for the cultivation of Bradyrhizobiurn CB756. Best results were obtained after dilution of the fermented ,medium to 10% (v/v) with a basal salts solution, but even with dilutions of 5 and 2.5%, high numbers of Bradyrhizobiurn CB756 cells were obtained.

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mentioning

confidence: 98%

Cultivation of Bradyrhizobium CB756 on sucrose prefermented by yeast

Lie¹,

Muilenburg²,

Hiep³

et al. 1992

Can. J. Microbiol.

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Influence of oxygenation and carbon dioxide on the growth ofRhizobium meliloti in a fermenter

Bourque

Groleau

1990

World J Microbiol Biotechnol

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A decrease in dissolved O2 from 90% to 50% saturation in a fermenter adversely affected both blomass production ofRhizobium meliloti ATCC 9930 and viable cell number, although oxygen was never limiting. Lower amounts of dissolved oxygen, or accidental decreases in dissolved oxygen concentration, also caused appreciable acidification of the culture broth, which was the result of CO2 accumulation in the medium. Adding CO2 to the aeration gas mixture affected both biomass production and mean generation time in proportion to the CO2 concentration; the effect on viable cell number was less pronounced.R. meliloti may be considered as a microorganism moderately sensitive to CO2. GoodR. meliloti growth requires, among other things, not only sufficient aeration (oxygenation) but also good ventilation of the CO2 evolved during the fermentation.

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Soil: Microbial Ecology

Wigginton¹,

Amador²

2020

Landscape and Land Capacity

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Aeration requirements ofRhizobium cultures

Cited by 3 publications

References 4 publications

Cultivation of Bradyrhizobium CB756 on sucrose prefermented by yeast

Cultivation of Bradyrhizobium CB756 on sucrose prefermented by yeast

Influence of oxygenation and carbon dioxide on the growth ofRhizobium meliloti in a fermenter

Soil: Microbial Ecology

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