Aerobic and Anaerobic Bacteria in Tonsils of Different Ages with Recurrent Tonsillitis

Karim, Gulbahar F.; AL-Salihi, Siham Sh.; Atya, Qanat Mahmood; Abass, Kasim Sakran

doi:10.5958/0976-5506.2019.02574.9

Cited by 2 publications

(2 citation statements)

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“…Staphylococcus aureus is a human skin and mucosa commensal. 1 It is a major pathogen found in both the community and hospitals that cause a variety of diseases, 2 including recurrent tonsillitis, 3 pneumonia, soft tissue infections, urinary tract infections, and bloodstream infections. 2 Although S. aureus can colonize various parts of the human body, the anterior nares are the principal ecological niche for this bacterium.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Methicillin-Resistant and Methicillin Sensitive Staphylococcus aureus Nasal Carriage and their Antibiotic Resistant Patterns in Kirkuk City, Iraq

AL-Salihi¹,

Karim²,

Al-Bayati³

et al. 2023

J. Pure Appl. Microbiol.

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The carriage of community-acquired methicillin-resistant S. aureus (CA-MRSA) has become a source of community-associated infections, particularly in the anterior nares of people worldwide. This study was aimed at determining the prevalence of community-acquired S. aureus (CA-SA) and CA-MRSA nasal carriage among healthy individuals of various ages in Kirkuk City and evaluating the susceptibility of isolates to various antibiotics. A total of 597 healthy individuals were recruited in the community between December 1, 2021, and December 30, 2022. Nasal swabs obtained from participants were taken to the laboratory, where bacteria were isolated and identified using phenotypic characteristics. The MRSA isolates were identified by applying the modified Kirby Bauer disc diffusion technique. The results showed that the prevalence of CA-SA and CA-MRSA nasal carriers was 16.6 and 4.5%, respectively. The average age of the participants was 33.2 years, with a male-to-female ratio of 1.1: 1. The highest resistance of the isolates was observed against oxacillin (27.3%), followed by penicillin G (24.3%), amoxicillin (15.2%), erythromycin (12.1%), and tetracycline (6.1%). There was a 9.1% resistance rate to clindamycin, rifampin, gentamycin, and ciprofloxacin. However, all CA-MRSA isolates were multi-drug resistant. However, all the isolates were sensitive (100 %) to vancomycin, linezolid, and mupirocin. The findings of the present study highlight the potential for CA-SA and CA-MRSA acquisition in this population, which may be related to antibiotic abuse or overuse as well as poor hygiene. To lessen the impact of community-associated strains of MRSA nasal carriage, this necessitates the probable need for infection prevention measures and adequate antibiotic therapy.

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Section: Introductionmentioning

confidence: 99%

Prevalence of Methicillin-Resistant and Methicillin Sensitive Staphylococcus aureus Nasal Carriage and their Antibiotic Resistant Patterns in Kirkuk City, Iraq

AL-Salihi¹,

Karim²,

Al-Bayati³

et al. 2023

J. Pure Appl. Microbiol.

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“…Antimicrobial therapy is frequently associated with the emergence of resistant bacteria, which is one of the largest problems and risks to global health. [1][2][3] Consequently, it is urgently needed to accelerate research and development for new antimicrobial strategies, 4 as well as for quick infection control during wound healing. 5 Laser radiation, a physical therapy modality with some advantages, including painlessness, noninvasiveness, and the ability to treat a variety of pathological diseases, has recently been used to control microbial activity.…”

Section: Introductionmentioning

confidence: 99%

Molecular Evaluation of the Impact of Nd:YAG Laser and Static Magnetic Field on Genomic DNA of Some Bacterial Isolates using RAPD-PCR

Atiyea¹,

Al-najar²,

Karim³

et al. 2022

J. Pure Appl. Microbiol.

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Antimicrobial therapy is frequently associated with the emergence of resistant bacteria with a high rate of morbidity and mortality worldwide. The present study was aimed at investigating the impact of a neodymium-doped yttrium aluminum (Nd:YAG) laser, and a static magnetic field (SMF) on cellular growth and DNA alteration in some clinical bacterial isolates. Samples from cutaneous wounds were collected by sterile cotton swabs from three elderly women admitted to Tikrit Teaching Hospital, Tikrit City, Iraq. Isolation and identification of Streptococcus agalactiae, Staphylococcus aureus, and Pseudomonas aeruginosa were carried out using cultural characteristics, microscopy, and biochemical tests. Three broth cultures were prepared for each of the test isolates. The first broth culture served as untreated control, the second was exposed to an Nd:YAG laser and the third was exposed to SMF. Colony counting was done on all the samples. DNA was extracted from the test bacteria and used to perform the RAPD-PCR assay. In contrast to the untreated control, the results showed that Nd:YAG laser radiation was more effective than SMF at inhibiting the cellular growth of the test isolates. Also, the radiation caused DNA alteration, which was established by decreased microbial growth, as well as the development of new bands and the loss of original bands. According to the findings of this study, the Nd:YAG laser is a promising technique for influencing the healing of infected cutaneous wounds. RAPD-PCR is also a useful biomarker assay for assessing the biological impact of laser radiation and SMF on bacteria.

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Aerobic and Anaerobic Bacteria in Tonsils of Different Ages with Recurrent Tonsillitis

Cited by 2 publications

References 0 publications

Prevalence of Methicillin-Resistant and Methicillin Sensitive Staphylococcus aureus Nasal Carriage and their Antibiotic Resistant Patterns in Kirkuk City, Iraq

Prevalence of Methicillin-Resistant and Methicillin Sensitive Staphylococcus aureus Nasal Carriage and their Antibiotic Resistant Patterns in Kirkuk City, Iraq

Molecular Evaluation of the Impact of Nd:YAG Laser and Static Magnetic Field on Genomic DNA of Some Bacterial Isolates using RAPD-PCR

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