Aerobic and Anaerobic Lactate Production in the Prepuberal and Adult Rat Testis

CHANGES OF (Ca2+-Mg2+)-ADENOSINETRIPHOSPHATASE ACTIVITY IN RAT TESTIS THROUGHOUT MATURATION

“…It may also suggest that during spermiogenesis, the testis progressively changes its energy metabolism until a different and stable pattern is attained in early maturity. In fact, previous studies have shown marked differences between the metabolic energy pattern of adult and immature testis in terms of Qp2 (Tepperman et al, 1949;Leider¬ man & Mancini, 1968), rate of glycolysis (Tepperman & Tepperman, 1950;Leiderman & Mancini, 1968), glucose utilization (Tepperman et al, 1949), ATP production (Means & Hall, 1968) and now ATP hydrolysis.…”

A simple and rapid technique to isolate enriched populations of spermatocytes and spermatids from the immature rat testis

“…Cell viability was determined by exclusion of 0-5 % trypan blue in a 1:1 (v/v) mixture of MEMS and Ca2+-and glucose-free Puck's saline G solution (137 mM-NaCl, 5-5 mM-KCl, 11 mM-KH2P04, 11 mM-Na2HP04 and 0-63 mMMgS04) and also by the ability of the cells to produce lactate aerobically compared with the ability of tissue fragments. Incubations were performed essentially as described by Leiderman (1969), and Leiderman & Mancini (1968), except that MEMS without fetal calf serum was used as the medium at 33°C. Separation.…”

INCREASE OF (Ca2+-Mg2+)-ADENOSINE TRIPHOSPHATASE ACTIVITY IN RAT TESTIS UNDERGOING REGRESSION OF THE GERMINAL EPITHELIUM

“…Testes undergoing regression of the germinal epithelium show a higher Qo2 (Tepperman, Tepperman & Dick, 1949;Featherstone, Nelson, Welden, Marberger, Boccabella & Boccabella, 1955;Steinberger & Wagner, 1961) and a lower glucose utilization (Tepperman & Tepperman, 1950;Means & Hall, 1968;Free, Massie & VanDemark, 1969) than normal testes. These phenomena are also observed in immature rat testes (Tepperman et al, 1949; Tepperman & Tepperman, 1950) and are believed to involve the intratubular tissue rather than the interstitium (Leiderman & Mancini, 1968).…”

“…Thus, hypophysectomy induces both regression of the germinal epithelium (Clermont & Morgentaler, 1955) and atrophy of the interstitium (Hooker, 1970). Cryptorchidism has a major effect upon the development of the germinal cells (Hooker, 1970 (Tepperman et al, 1949;Featherstone et al, 1955;Steinberger & Wagner, 1961 ;Leiderman & Mancini, 1968), glucose utilization (Tepperman & Tepperman, 1950;Means & Hall, 1968;Free et al, 1969), UDPase activity (Xuma & Turkington, 1972) …”