Aerosol Transfer of Bladder Urothelial and Smooth Muscle Cells Onto Demucosalized Colonic Segments: A Pilot Study

Abstract

Separation, cell suspension and aerosol delivery of bladder urothelial and smooth muscle cells in fibrin glue can successfully transfer these urological cell populations to a new host tissue commonly used in urological reconstruction. In vivo co-culture of bladder smooth muscle and urothelial cells results in coverage of a large area of demucosalized gut providing new potential for transfer and reconstitution of urologically functionally appropriate tissue to the bladder itself.

The use of seromuscular tapered ileal tube in ureteral replacement: an experimental model

“…The results of seromuscular colocystoplasty have been encouraging and comparable with those of the standard augmentation technique [3]. In experimental animals of demucosalized colonic patch in dogs, severe contraction of the graft was observed [16]. Demucosalized intestinal segments have been complicated by shrinkage and regrowth of the original gastrointestinal epithelium [17].…”

“…In one experimental animal, we used this method, and the graft could successfully survive, with minimal changes in the length and caliber of the tube; however, further research should still be carried on a series of animals to settle these findings. Recently, cell transfer technology for transferal cells suspension of bladder urothelium with fibrin glue has been tried on demucosalized gut [16], with successful early results.…”

“…Mucus secretion increases the viscosity of urine and hinders urine transport and emptying [3], mucus also may contribute to the persistent bacteriuria and stone formation [4,5]. The reabsorption of ammonium from the urine resulted in metabolic acidosis [2][3][4][5][6]; moreover, a higher incidence of malignant transformation of the intestinal mucosa has been reported [7][8][9][10][11][12][13][14][15][16][17][18].…”

Step‐by‐Step Build‐Up of Biologically Active Cell‐Containing Stratified Films Aimed at Tissue Engineering

“…[12] Indeed, this deposition method has been previously used to spray keratinocytes on skin wounds to accelerate healing, [13] melanocytes to restore skin color, [14] and to deliver bladder urothelial and smooth muscle cells to reconstitute colon segments. [15] The layer-by-layer (LBL) buildup of PEM films from oppositely charged polyelectrolytes offers new opportunities for the preparation of functionalized biomaterial coatings by the dipping [16] and spraying methods. [17,18] This technique allows the preparation of supramolecular nanoarchitectures exhibiting specific properties in terms of control of cell activation, [19][20][21][22][23] and may also play a role in the development of local-drug-delivery systems.…”

Seromuscular Grafts for Bladder Reconstruction: Extra-luminal Demucosalisation of the Bowel

“…In subsequent work, they used a Foley catheter inside the lumen to facilitate dissection and then retrieved the mucosa and excised it via the rectum 11 . Hafez et al 12 adapted this technique, but instead used hydrodistension of the colonic segment to facilitate the extraluminal dissection. Here, we present results of extraluminal demucosalization with an indwelling Foley catheter.…”