Affinity capture using peptide-functionalized magnetic nanoparticles to target Staphylococcus aureus

Kuo, Fang Yin; Lin, Wei Lien; Chen, Yu-Chie

doi:10.1039/c6nr00368k

Cited by 26 publications

(16 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported, Hussain et al (2018) connected vancomycin-loaded nanoparticles with the cyclic 9-amino-acid peptide CARGGLKSC (CARG) which could specifically bind to S. aureus , realizing more effective suppression of staphylococcal infections in vivo than untargeted treatment ( Figure 3B ). Moreover, magnetic nanoparticles (MNPs) could also serve as affinity probes to selectively enrich S. aureus with peptide HHHHHHDEEGLFVD (D) due to special properties ( Kuo et al, 2016 ). On this basis, improvements lie in the addition of outer package to maintain long effects.…”

Section: Nanomaterial-based Targeted Therapymentioning

confidence: 99%

Targeted Therapeutic Strategies in the Battle Against Pathogenic Bacteria

Yang

Fang

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

The emergence and rapid spread of antibiotic resistance in pathogenic bacteria constitute a global threat for public health. Despite ongoing efforts to confront this crisis, the pace of finding new potent antimicrobials is far slower than the evolution of drug resistance. The abuse of broad-spectrum antibiotics not only accelerates the formation of resistance but also imposes a burden on the intestinal microbiota, which acts a critical role in human homeostasis. As such, innovative therapeutic strategies with precision are pressingly warranted and highly anticipated. Recently, target therapies have achieved some breakthroughs by the aid of modern technology. In this review, we provide an insightful illustration of current and future medical targeted strategies, including narrow-spectrum agents, engineered probiotics, nanotechnology, phage therapy, and CRISPR-Cas9 technology. We discuss the recent advances and potential hurdles of these strategies. Meanwhile, the possibilities to mitigate the spread of resistance in these approaches are also mentioned. Altogether, a better understanding of the advantages, disadvantages, and mechanisms of action of these targeted therapies will be conducive to broadening our horizons and optimizing the existing antibacterial approaches.

show abstract

Section: Nanomaterial-based Targeted Therapymentioning

confidence: 99%

Targeted Therapeutic Strategies in the Battle Against Pathogenic Bacteria

Yang

Fang

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Jo et al used nano-MNPs for catching free cancer cells [ 21 ]. Kuo et al [ 22 ] reported biofunctional MNPs for pathogen separation and concentration. MNPs are also utilized for purifying target bacteria and increasing their count to allow the easy bacterial identification [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Vancomycin-Loaded Furriness Amino Magnetic Nanospheres for Rapid Detection of Gram-Positive Water Bacterial Contamination

et al. 2022

View full text Add to dashboard Cite

Bacterial pathogens pose high threat to public health worldwide. Different types of nanomaterials have been synthesized for the rapid detection and elimination of pathogens from environmental samples. However, the selectivity of these materials remains challenging, because target bacterial pathogens commonly exist in complex samples at ultralow concentrations. In this study, we fabricated novel furry amino magnetic poly-L-ornithine (PLO)/amine-poly(ethylene glycol) (PEG)-COOH/vancomycin (VCM) (AM-PPV) nanospheres with high-loading VCM for vehicle tracking and the highly efficient capture of pathogens. The magnetic core was coated with organosilica and functionalized with cilia. The core consisted of PEG/PLO loaded with VCM conjugated to Gram-positive bacterial cell membranes, forming hydrogen bonds with terminal peptides. The characterization of AM-PPV nanospheres revealed an average particle size of 56 nm. The field-emission scanning electron microscopy (FE-SEM) micrographs showed well-controlled spherical AM-PPV nanospheres with an average size of 56 nm. The nanospheres were relatively rough and contained an additional 12.4 nm hydrodynamic layer of PLO/PEG/VCM, which provided additional stability in the suspension. The furry AM-PPV nanospheres exhibited a significant capture efficiency (>90%) and a high selectivity for detecting Bacillus cereus (employed as a model for Gram-positive bacteria) within 15 min, even in the presence of other biocompatible pathogens. Moreover, AM-PPV nanospheres rapidly and accurately detected B. cereus at levels less than 10 CFU/mL. The furry nano-design can potentially satisfy the increasing demand for the rapid and sensitive detection of pathogens in clinical and environmental samples.

show abstract

“…The combination of aptamer-based capture/enrichment and MS analysis of microorganisms takes advantage of the selectivity of both techniques and should enhance the accuracy of microbial identification. A peptide aptamer that recognizes both SA and MRSA has been immobilized on gold nanoparticles to capture the bacteria prior to surface-assisted laser desorption ionization-MS analysis [ 38 , 39 ]. The present study aims to develop an affinity mass spectrometric method for selective analysis of MRSA.…”

Section: Introductionmentioning

confidence: 99%

Selective Capture and Identification of Methicillin-Resistant Staphylococcus aureus by Combining Aptamer-Modified Magnetic Nanoparticles and Mass Spectrometry

Liu

Katragunta

et al. 2021

IJMS

View full text Add to dashboard Cite

A nucleic acid aptamer that specifically recognizes methicillin-resistant Staphylococcus aureus (MRSA) has been immobilized on magnetic nanoparticles to capture the target bacteria prior to mass spectrometry analysis. After the MRSA species were captured, they were further eluted from the nanoparticles and identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The combination of aptamer-based capture/enrichment and MS analysis of microorganisms took advantage of the selectivity of both techniques and should enhance the accuracy of MRSA identification. The capture and elution efficiencies for MRSA were optimized by examining factors such as incubation time, temperature, and elution solvents. The aptamer-modified magnetic nanoparticles showed a capture rate of more than 90% under the optimized condition, whereas the capture rates were less than 11% for non-target bacteria. The as-prepared nanoparticles exhibited only a 5% decrease in the capture rate and a 9% decrease in the elution rate after 10 successive cycles of utilization. Most importantly, the aptamer-modified nanoparticles revealed an excellent selectivity towards MRSA in bacterial mixtures. The capture of MRSA at a concentration of 102 CFU/mL remained at a good percentage of 82% even when the other two species were at 104 times higher concentration (106 CFU/mL). Further, the eluted MRSA bacteria were successfully identified using MALDI mass spectrometry.

show abstract

Affinity capture using peptide-functionalized magnetic nanoparticles to target Staphylococcus aureus

Cited by 26 publications

References 53 publications

Targeted Therapeutic Strategies in the Battle Against Pathogenic Bacteria

Targeted Therapeutic Strategies in the Battle Against Pathogenic Bacteria

Vancomycin-Loaded Furriness Amino Magnetic Nanospheres for Rapid Detection of Gram-Positive Water Bacterial Contamination

Selective Capture and Identification of Methicillin-Resistant Staphylococcus aureus by Combining Aptamer-Modified Magnetic Nanoparticles and Mass Spectrometry

Contact Info

Product

Resources

About