Affinity of Serum Albumin and Fibrinogen to Cellulose, Its Hydrophobic Derivatives and Blends

Kargl, Rupert; Bračič, Matej; Resnik, Matic; Mozetič, Miran; Bauer, Wolfgang; Kleinschek, Karin Stana; Mohan, Tamilselvan

doi:10.3389/fchem.2019.00581

Cited by 9 publications

(3 citation statements)

References 37 publications

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“…The protein layer may moderate biocompatibility via activation of the blood coagulation cascade, which affects the lifetime of the biomaterial. Multiple strategies have been developed to control protein adsorption, platelet activation, and aggregate formation when the blood comes in contact with medical devices. ,,− Among them, surface functionalization with thin ceramic coatings offers a promising approach.…”

Section: Discussionmentioning

confidence: 99%

Rolling or Two-Stage Aggregation of Platelets on the Surface of Thin Ceramic Coatings under in Vitro Simulated Blood Flow Conditions

Mzyk

Imbir

Trembecka-Wójciga

et al. 2020

ACS Biomater. Sci. Eng.

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The process of modern cardiovascular device fabrication should always be associated with an investigation of how surface properties modulate its hemocompatibility through plasma protein adsorption as well as blood morphotic element activation and adhesion. In this work, a package of novel assays was used to correlate the physicochemical properties of thin ceramic coatings with hemocompatibility under dynamic conditions. Different variants of carbon-based films were prepared on polymer substrates using the magnetron sputtering method. The microstructural, mechanical, and surface physicochemical tests were performed to characterize the coatings, followed by investigation of whole human blood quality changes under blood flow conditions using the “Impact R” test, tubes’ tester, and radial flow chamber assay. The applied methodology allowed us to determine that aggregate formation on hydrophobic and hydrophilic carbon-based coatings may follow one of the two different mechanisms dependent on the type and conformational changes of adsorbed blood plasma proteins.

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Section: Discussionmentioning

confidence: 99%

Rolling or Two-Stage Aggregation of Platelets on the Surface of Thin Ceramic Coatings under in Vitro Simulated Blood Flow Conditions

Mzyk

Imbir

Trembecka-Wójciga

et al. 2020

ACS Biomater. Sci. Eng.

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“…[53] Alternatively, changes in surface hydrophobicity at different PU-PEG concentrations may contribute to the improved nonfouling ability at higher concentrations. [54][55][56][57] Serum proteins typically adsorb more efficiently onto hydrophobic surfaces, [54,58,59] but the greater charge density of albumin may allow it to bind to more hydrophilic surfaces than fibrinogen. Increased density of presented PEG chains is also widely implicated in reducing protein adsorption.…”

Section: Nonfouling Characteristics and Implant Tolerability Of Pu-pe...mentioning

confidence: 99%

Tailored Biocompatible Polyurethane‐Poly(ethylene glycol) Hydrogels as a Versatile Nonfouling Biomaterial

Speidel

Wood

Roberts

et al. 2022

Adv Healthcare Materials

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Polyurethane-based hydrogels are relatively inexpensive and mechanically robust biomaterials with ideal properties for various applications, including drug delivery, prosthetics, implant coatings, soft robotics, and tissue engineering. In this report, a simple method is presented for synthesizing and casting biocompatible polyurethane-poly(ethylene glycol) (PU-PEG) hydrogels with tunable mechanical properties, nonfouling characteristics, and sustained tolerability as an implantable material or coating. The hydrogels are synthesized via a simple one-pot method using commercially available precursors and low toxicity solvents and reagents, yielding a consistent and biocompatible gel platform primed for long-term biomaterial applications. The mechanical and physical properties of the gels are easily controlled by varying the curing concentration, producing networks with complex shear moduli of 0.82-190 kPa, similar to a range of human soft tissues. When evaluated against a mechanically matched poly(dimethylsiloxane) (PDMS) formulation, the PU-PEG hydrogels demonstrated favorable nonfouling characteristics, including comparable adsorption of plasma proteins (albumin and fibrinogen) and significantly reduced cellular adhesion. Moreover, preliminary murine implant studies reveal a mild foreign body response after 41 days. Due to the tunable mechanical properties, excellent biocompatibility, and sustained in vivo tolerability of these hydrogels, it is proposed that this method offers a simplified platform for fabricating soft PU-based biomaterials for a variety of applications.

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“…Adsorption of proteins (e.g., albumins) is expected in untreated porous materials such as the absorbent material, which likely accounts for the observed loss. We measured the concentration of total protein eluted from each filter layer of the device (i.e., prefilter material only and PSM only) to determine where the proteins were being adsorbed . We found no significant loss to either filter material (SI Figure S2B), which may indicate that only the absorbent material is responsible for the slight loss of total protein.…”

Section: Resultsmentioning

confidence: 96%

High-Yielding Separation and Collection of Plasma from Whole Blood Using Passive Filtration

et al. 2020

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Lateral flow tests and hand-held analyzers facilitate diagnostic testing in resource limited settings and at the point-of-care. However, many of these devices require sample preparation such as plasma separation to remove cells and isolate the liquid portion of blood. Specifically, the separation of plasma from blood is necessary for routine health assessments such as comprehensive metabolic panels and chronic HIV viral load monitoring. Away from laboratories, this type of processing has been addressed by unconventional, hand-operated centrifuge devices (high volume) or plasma separation membranes (PSM) coupled with lateral flow tests (low volume). Herein, we describe a device that separates and stores plasma from undiluted blood using only passive filtration in less than 10 min. Integrating a PSM with a prefilter and absorbent material yields a 3-fold increase in separation efficiency compared to similar devices using passive filtration. We demonstrate the reproducibility of our device across the physiological range of hematocrits (20−50%) with an average recovered plasma volume of 61.7 ± 2.6 μL. Maximum separation efficiency (53.8%, 65.6 ± 3.9 μL plasma) was achieved for a sample of whole blood (30% hematocrit) in 10 min. We evaluate the purity of our plasma sample by quantitation of hemoglobin and report hemolysis as either minimal (≤5%) or undetectable (≤1%). Specific recovery of human IgG, IFN-γ, and HIV-1 RNA indicate the diagnostic utility of plasma obtained from our device is unchanged compared to plasma obtained via centrifugation. Finally, we demonstrate the use of recovered plasma, applied via "stamping", to successfully conduct a commercial lateral flow immunochromatographic assay for tetanus antibodies. This device platform is capable of producing pure plasma samples from blood to facilitate tests in resource limited settings to improve access to healthcare.

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Affinity of Serum Albumin and Fibrinogen to Cellulose, Its Hydrophobic Derivatives and Blends

Cited by 9 publications

References 37 publications

Rolling or Two-Stage Aggregation of Platelets on the Surface of Thin Ceramic Coatings under in Vitro Simulated Blood Flow Conditions

Rolling or Two-Stage Aggregation of Platelets on the Surface of Thin Ceramic Coatings under in Vitro Simulated Blood Flow Conditions

Tailored Biocompatible Polyurethane‐Poly(ethylene glycol) Hydrogels as a Versatile Nonfouling Biomaterial

High-Yielding Separation and Collection of Plasma from Whole Blood Using Passive Filtration

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