Affinity sorbents containing nucleic acids and their fragments

Abstract

The published data on the main methods for the The published data on the main methods for the preparation of polymeric supports containing nucleic acids (NA) preparation of polymeric supports containing nucleic acids (NA) or their fragments (oligonucleotides) are reviewed with special or their fragments (oligonucleotides) are reviewed with special emphasis on chemical immobilisation. Some physical and phys-emphasis on chemical immobilisation. Some physical and physicochemical immobilisation techniques, including those based on icochemical immobilisation techniques, including those based on the use of enzymes and avidin ± biotin interactions and prepara-the use of enzymes and avidin ± biotin interactions and preparation of NA-containing supports by direct oligonucleotide syn-tion of NA-containing supports by direct oligonucleotide synthesis on these supports are considered. A special section is thesis on these supports are considered. A special section is devoted to the application of NA-containing sorbents for the devoted to the application of NA-containing sorbents for the isolation of individual NA and proteins as well as in hybridisation isolation of individual NA and proteins as well as in hybridisation analysis including those utilising DNA chips and DNA biosen-analysis including those utilising DNA chips and DNA biosensors. The bibliography includes 391 references sors. The bibliography includes 391 references. .

Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces

“…There are comprehensive reviews outlining different approaches to oligonucleotide immobilization for fabrication of DNA chips [1][2][3]. Most methods describe the covalent attachment of isolated and purified oligonucleotides bearing different functional groups to the modified solid support (e.g., [4][5][6][7][8][9][10]).…”