AFLP analyses of genetic variation of Eupatorium adenophorum (Asteraceae) populations in China

Abstract

. 2009. AFLP analyses of genetic variation of Eupatorium adenophorum (Asteraceae) populations in China. Can. J. Plant Sci. 89: 119Á126. The Eupatorium adenophorum is one of the most widespread invasive alien species in China. In the present study, the genetic variation and population structure of this species were analyzed using amplified fragment length polymorphism (AFLP) markers. Nine primer pairs were selected for the analysis and 685 bands were produced, among which 474 bands were polymorphic (PPB 069.2%). Diversity levels within populations were relatively high (mean expected heterozygosity 00.188, mean Shannon index 0 0.296). Regression analysis showed a significant positive relationship between Shannon genetic diversity and altitude (R 2 0 0.31). However, there was a negative correlation between Shannon genetic diversity and latitude (R 2 00.16), as well as between Shannon genetic diversity and longitude (R 2 00.45). Cluster analysis grouped the majority of the weed populations into three main clusters that corresponded with the geographic regions. At the regional level, the AMOVA indicated that about 70% of the variations in the data set were from genotypic variations within populations, 13.3% of the variations were due to regional differences, and the remaining 16.6% were due to differences among populations within the provincial regions. The results imply that most individuals tested in the present study should have been produced through seeds, and the process of colonization resulted in progressive loss of genetic diversity from the southwest to the northeast of China. Eupatorium adenophorum est une des espe`ces exotiques envahissantes les plus re´pandues en Chine. La pre´sente e´tude devait e´tablir la variation ge´ne´tique et la distribution de la population de cette espe`ce. Pour cela, les auteurs ont recouru a`l'analyse de marqueurs par polymorphisme amplifie´de la longueur des fragments (AFLP). Neuf paires d'amorces ont e´te´retenues pour l'analyse et 685 bandes ont e´te´obtenues, dont 474 polymorphes (PPB 069.2 %). Le degre´de diversite´des populations est relativement e´leve´(he´te´rozygotie moyenne pre´vue00.188, indice moyen de Shannon 00.296). L'analyse de re´gression re´ve`le l'existence d'un lien positif significatif entre l'indice de diversite´ge´ne´tique de Shannon et l'altitude (R 2 00.31); cependant, il existe une corre´lation ne´gative entre cet indice et la latitude (R 2 00.16) ainsi que la longitude (R 2 00.45). L'analyse par grappes rassemble la majorite´des populations de l'adventice en trois vastes groupes correspondant aux zones ge´ographiques. Sur le plan re´gional, l'analyse de la variance mole´culaire indique qu'environ 70 % des fluctuations dans les donne´es re´sultent de la variation ge´notypique au sein des populations, 13,3 % de diffe´rences re´gionales et 16,6 % de diffe´rences entre les populations des provinces. On en de´duit que la plupart des plantes teste´es dans le cadre de cette e´tude auraient duˆeˆtre cultive´es a`partir de semences et que la colonisation a ent...

Hybridization of an invasive shrub affects tolerance and resistance to defoliation by a biological control agent

“…The abundance of tamarisk hybrids in North America compared to the lack of interspecific hybridization in the native range in Asia suggests that species introgression has played an important role for invasion success (Gaskin and Kazmer 2009;Gaskin et al 2012). Similar patterns of latitude and genetic variation are demonstrated by at least one other invasive shrub, Eupatorium adenophorum (Huang et al 2009).…”

Invasive mechanism and control strategy of Ageratina adenophora (Sprengel)

“…From there, the populations in western Yunnan spread northward to southern Sichuan, and further dispersed to the Yangtze River valley in Sichuan by water flow. Several southeastern populations spread eastward to northwestern Guangxi Province, and others spread northward to the central part of Yunnan and northeastward to the western part of Guizhou Province [20,14,[17][18][19]. The ISSR-PCR analysis with the individuals/populations of A. adenophora detected intra-specific polymorphism of nuclear DNA, however, the PCR-RFLP analysis of 256 individuals revealed no variations in cDNA in the Chinese populations.…”

“…The results showed that it has many geographical populations in China, and genetic differentiations occurred within those populations. Inter-simple sequence repeat (ISSR) markers were used to assess the bio-geographic relationships among 64 populations of A. adenophora during 2004-2006 in China and Neighbor-joining (NJ) cluster analysis based on genetic distances among the populations grouped the populations according to their geographical origin, i.e., populations of Southwestern Guizhou, populations of Liangshan city in Sichuan, populations of Western Guizhou, populations of Guangxi plus Chongqing, populations of Southern Yunnan, and populations of Yangtze River Valleys in Sichuan plus western Yunnan , environmental isolation is the primary reason of for the genetic differentiation of the alien species [13][14][15][16][17][18][19].…”

Biology and management of the invasive weed Ageratina adenophora (Asteraceae): current state of knowledge and future research needs

“…Research carried out by using intersimple sequence repeat (ISSR) markers and amplified fragment length polymorphism (AFLP) method in China exhibited genetic variations in the population of A. adenophora (Gui et al ., ; Huang et al ., ). This genetic diversity or genetic distance increased with increasing geographic distance and elevation (Gui et al ., ; Huang et al ., ). More genetic heterogeneity within populations of A. adenophora explains the possibility of multiple introduction in China (Huang et al ., ).…”