Agarose-based biomaterials for tissue engineering

Zarrintaj, Payam; Manouchehri, Saeed; Ahmadi, Zahed; Saeb, Mohammad Reza; Urbanska, Aleksandra M.; Kaplan, David L.; Mozafari, Masoud

doi:10.1016/j.carbpol.2018.01.060

Cited by 540 publications

(329 citation statements)

References 210 publications

Supporting

Mentioning

327

Contrasting

Order By: Relevance

“…Agarose is a co‐polymer composed of alternating moieties of (1,3)‐linked b‐ d ‐galactopyranose and (1,4)‐linked 3,6‐anhydro‐a‐ l ‐galactopyranose. Due to its favorable bio‐profile including nontoxicity, expansive range of biocompatibility, and low nonspecific absorptivity, agarose hydrogels have been extensively implemented in a wide variety of biological applications, including for the cultivation, immobilization, and testing of a diverse population of cell models (Lozinsky et al, ; Zarrintaj et al, ). Increasingly, agarose cryogels are being used in tissue engineering applications; an example is an application of agarose cryogel supermacroporous bioscaffolds used for culturing insulin‐producing cells (Bloch et al, ).…”

Section: Cryogel Biomaterialsmentioning

confidence: 99%

Three‐dimensional cryogels for biomedical applications

Razavi

Qiao

Thakor

2019

J Biomedical Materials Res

View full text Add to dashboard Cite

Cryogels are a subset of hydrogels synthesized under sub‐zero temperatures: initially solvents undergo active freezing, which causes crystal formation, which is then followed by active melting to create interconnected supermacropores. Cryogels possess several attributes suited for their use as bioscaffolds, including physical resilience, bio‐adaptability, and a macroporous architecture. Furthermore, their structure facilitates cellular migration, tissue‐ingrowth, and diffusion of solutes, including nano‐ and micro‐particle trafficking, into its supermacropores. Currently, subsets of cryogels made from both natural biopolymers such as gelatin, collagen, laminin, chitosan, silk fibroin, and agarose and/or synthetic biopolymers such as hydroxyethyl methacrylate, poly‐vinyl alcohol, and poly(ethylene glycol) have been employed as 3D bioscaffolds. These cryogels have been used for different applications such as cartilage, bone, muscle, nerve, cardiovascular, and lung regeneration. Cryogels have also been used in wound healing, stem cell therapy, and diabetes cellular therapy. In this review, we summarize the synthesis protocol and properties of cryogels, evaluation techniques as well as current in vitro and in vivo cryogel applications. A discussion of the potential benefit of cryogels for future research and their application are also presented.

show abstract

Section: Cryogel Biomaterialsmentioning

confidence: 99%

Three‐dimensional cryogels for biomedical applications

Razavi

Qiao

Thakor

2019

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…In parallel to PDMS-based microsystems, different hydrogel-based approaches were also developed [34], including silk [35], alginate [36], polyacrylamide [37] or Poly(ethylene glycol) diacrylate(PEGDA)derived microsystems [38]. Among them, agarose was uniquely shown to cover a wide range of physiological stiffness, achieved by tuning the type and percentage of agarose [39]. The agarose matrix allows the free diffusion of salt and small molecules (size < 30 nm in 2% agarose [40], which is the case for most proteins), ensuring passive medium renewal.…”

Section: Introductionmentioning

confidence: 99%

“…The agarose matrix allows the free diffusion of salt and small molecules (size < 30 nm in 2% agarose [40], which is the case for most proteins), ensuring passive medium renewal. While it is currently widely used in tissue engineering [39], it has however only been implemented in microfluidic systems by a limited number of groups [41][42][43]. The main limitation for its wide-use in lab-on-chip applications is its difficult integration in user-friendly protocols enabling easy sealing and cell recovery.…”

Section: Introductionmentioning

confidence: 99%

Development of a soft cell confiner to decipher the impact of mechanical stimuli on cells

Prunet

Lefort

Delanoë-Ayari

et al. 2020

Preprint

View full text Add to dashboard Cite

Emerging evidence suggests the importance of mechanical stimuli in normal and pathological situations for the control of many critical cellular functions. While the effect of matrix stiffness has been and is still extensively studied, few studies have focused on the role of mechanical stresses. The main limitation of such analyses is the lack of standard in vitro assays enabling extended mechanical stimulation compatible with dynamic biological and biophysical cell characterization. We have developed an agarose-based microsystem, the soft cell confiner, which enables the precise control of confinement for single or mixed cell populations. The rigidity of the confiner matches physiological conditions and enables passive medium renewal. It is compatible with time-lapse microscopy, in situ immunostaining, and standard molecular analyses, and can be used with both adherent and nonadherent cell lines. Cell proliferation of various cell lines (hematopoietic cells, MCF10A epithelial breast cells and HS27A stromal cells) was followed for several days up to confluence using video-microscopy and further documented by Western blot and immunostaining. Interestingly, even though the nuclear projected area was much larger upon confinement, with many highly deformed nuclei (non-circular shape), cell viability, assessed by live and dead cell staining, was unaffected for up to 8 days in the confiner. However, there was a decrease in cell proliferation upon confinement for all tested cell lines. The soft cell confiner is thus a valuable tool to decipher the effect of long-term confinement and deformation on the biology of cell populations. This tool will be instrumental in deciphering the impact of nuclear and cytoskeletal mechanosensitivity in normal and pathological conditions involving highly confined situations, such as those reported upon aging with fibrosis or during cancer. Highlights-We have developed a flexible hydrogel-based microsystem that precisely confines cells for extended periods of time with controlled nutrient levels.-The soft confiner system was validated for both adherent and non-adherent cells and allows the simultaneous in situ follow-up or ex situ analysis of multiple subpopulations.-A unique tool to analyze the role of long-term effect of mechanical confinement in normal and pathological conditions. Graphical abstract3/27

show abstract

“…Among different methods, nonwoven nanofiber mats can be fabricated by electrospinning technique as a simple and useful skill in which fibers diameter can be altered from microns to nanometers . Various natural and synthetic polymers such as chitosan, agarose, starch, Poly(lactic acid) PLA, polycaprolactone (PCL) have been used to fabricate the electrospun nanofibers . In this regards, wide ranges of properties such as enriched mechanical/thermal properties, conductivity and porosity can be achieved by the selection of proper materials.…”

Section: Introductionmentioning

confidence: 99%

Electrospun electroactive nanofibers of gelatin‐oligoaniline/Poly (vinyl alcohol) templates for architecting of cardiac tissue with on‐demand drug release

Shojaie

Rostamian

Samadi

et al. 2019

Polymers for Advanced Techs

Self Cite

View full text Add to dashboard Cite

In this study, grafted gelatin with oligoaniline (GelOA) was synthesized and then mixed with Poly (vinyl alcohol) (PVA). Several scaffolds with different ratio of PVA/GelOA were electrospun to fabricate electroactive scaffolds. GelOA was characterized using Fourier‐transform infrared spectroscopy (FTIR); moreover, nanofiber properties were evaluated by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and scanning electron microscope (SEM) analyses. Nanofibers diameter was decreased with aniline oligomer increment form 300 to 150 nm because of the hydrophobic nature of the aniline oligomer. Aniline oligomer electroactivity was studied using cyclic voltammetry, which exhibited two redox peaks at 0.4 and 0.6. Moreover, aniline oligomer enhancement resulted in melting point increasing from 220°C to 230°C because of the crystallinity increment. To assess the biocompatibility of nanofibers, cell viability and cell adhesion were tracked using mesenchymal stem cell (MSCs). It was revealed that the presence of aniline oligomer leads to enhancing the conductivity, thermal properties and lowering the degradation rate and drug release. Among of different scaffolds, sample with high content of GelOA shows better behavior in physical and biological properties. Accumulative drug releases under applied electrical field at 40 minutes showed that the drug release for stimulated condition is about 33% more than the unapplied electrical field one.

show abstract

Agarose-based biomaterials for tissue engineering

Cited by 540 publications

References 210 publications

Three‐dimensional cryogels for biomedical applications

Three‐dimensional cryogels for biomedical applications

Development of a soft cell confiner to decipher the impact of mechanical stimuli on cells

Electrospun electroactive nanofibers of gelatin‐oligoaniline/Poly (vinyl alcohol) templates for architecting of cardiac tissue with on‐demand drug release

Contact Info

Product

Resources

About