Age-dependent changes in the structure, composition and biophysical properties of a human basement membrane

Candiello, Joseph; Cole, Gregory J.; Halfter, Willi

doi:10.1016/j.matbio.2010.03.004

Cited by 163 publications

(195 citation statements)

References 56 publications

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“…Indeed, proteins such as fibulin might play an ongoing role in shaping and maintaining tissues (Kubota et al, 2012). In addition, basement membranes show dramatic changes in structure with age (Candiello et al, 2010;Danysh et al, 2008;Khalil-Manesh and Price, 1985;Taylor and Price, 1982). It will be interesting to determine whether age-dependent alterations to basement membranes underlie any changes the structure and function of aged tissues.…”

Section: Discussionmentioning

confidence: 99%

An active role for basement membrane assembly and modification in tissue sculpting

Morrissey

Sherwood

2015

Journal of Cell Science

121

105

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Basement membranes are a dense, sheet-like form of extracellular matrix (ECM) that underlie epithelia and endothelia, and surround muscle, fat and Schwann cells. Basement membranes separate tissues and protect them from mechanical stress. Although traditionally thought of as a static support structure, a growing body of evidence suggests that dynamic basement membrane deposition and modification instructs coordinated cellular behaviors and acts mechanically to sculpt tissues. In this Commentary, we highlight recent studies that support the idea that far from being a passive matrix, basement membranes play formative roles in shaping tissues.

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Section: Discussionmentioning

confidence: 99%

An active role for basement membrane assembly and modification in tissue sculpting

Morrissey

Sherwood

2015

Journal of Cell Science

121

105

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“…The underlining reason still need further research to explain this contradiction. Meanwhile, we can just find low concentration of DEXP in the vitreous, maybe owning to the integrated internal limiting membrane, a basement membrane, which acts as a border between the vitreous humor and the neuroretina (Dalkara et al, 2009;Candiello et al, 2010). Overall, the high concentrations of the DEXP in the choroids and retina ensure that the periocular capsular DDS can act as an alternative choice to the posterior drug delivery.…”

Section: Discussionmentioning

confidence: 99%

A study of the dexamethasone sodium phosphate release properties from a periocular capsular drug delivery system

et al. 2014

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The aim of this study was to investigate whether a periocular capsular drug delivery system (DDS) can release dexamethasone sodium phosphate (DEXP) in vitro and in vivo to the posterior segment of rabbit's eye. In vitro, the periocular capsular DDS containing 2 mg/ml or 5 mg/ml DEXP was immersed in modified Franz diffusion cell. Four-hundred microliters of liquid was aspirated at 0.5, 1, 2, 4, 8, 24 and 48 h for determination. In vivo, the DEXP-filled periocular capsular DDS was implanted into the sub-Tenon's sac of the New Zealand rabbit. DEXP concentration at the serum aqueous humor, cornea, iris, lens, ciliary body, vitreous, retina, choroids and sclera was quantified at 1, 3, 7, 14, 28 and 56 d after implantation. The DEXP concentration was determined by ultra-performance liquid chromatography-tandem mass spectrometry. In vitro, the periocular capsular DDS released the DEXP in time-dependent manner from 1/2 to 48 h. In vivo, the concentrations of the DEXP at the retina, choroids, ciliary body and iris were 123.11 (91.23, 732.61) ng/g, 362.46 ± 330.46 ng/g, 71.64 (71.35, 180.21) ng/g and 192.50 ± 42.66 ng/g, respectively, at 56 d after implantation. Minimal DEXP was found in the aqueous, serum and vitreous. Our results demonstrated that DEXP could be sustained released from the periocular capsular DDS, which indicated that the periocular capsular DDS might be a potential candidate of transscleral drug delivery for the management of posterior segment diseases.

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“…Forceindentation measurements by atomic force microscopy also showed that ILMs become increasingly stiffer with advancing age. 29 …”

Section: Basal Membranementioning

confidence: 99%

“…79,92 Pericytes are uniquely positioned within the NVU between ECs of brain capillaries, astrocytes and neurons. Winkler et al have reviewed the concept of the NVU and neurovascular functions of CNS pericytes, discussing vascular contributions to Alzheimer's disease and new roles of pericytes in the pathogenesis of 72 Hicks et al 44 Burns et al 49 Hunziker et al 72 Burns et al 49,50 Grammas et al 18 Tight junctions Expression of tight junction proteins: decreased Elahy et al, 71 Enciu et al 73 Basal lamina Thickness of basement membrane: Increased Concentration of collagen IV and argin: Increased Concentration of laminin: decreased Candiello et al, 29 Ravens 74 Candiello et al 29 Candiello et al 29 …”

Section: Bbb In Alzheimer's Diseasementioning

confidence: 99%

Age-associated physiological and pathological changes at the blood–brain barrier: A review

Erdő

Dénès

Lange

2016

J Cereb Blood Flow Metab

352

260

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The age-associated decline of the neurological and cognitive functions becomes more and more serious challenge for the developed countries with the increasing number of aged populations. The morphological and biochemical changes in the aging brain are the subjects of many extended research projects worldwide for a long time. However, the crucial role of the blood-brain barrier (BBB) impairment and disruption in the pathological processes in age-associated neurodegenerative disorders received special attention just for a few years. This article gives an overview on the major elements of the blood-brain barrier and its supporting mechanisms and also on their alterations during development, physiological aging process and age-associated neurodegenerative disorders (Alzheimer's disease, multiple sclerosis, Parkinson's disease, pharmacoresistant epilepsy). Besides the morphological alterations of the cellular elements (endothelial cells, astrocytes, pericytes, microglia, neuronal elements) of the BBB and neurovascular unit, the changes of the barrier at molecular level (tight junction proteins, adheres junction proteins, membrane transporters, basal lamina, extracellular matrix) are also summarized. The recognition of new players and initiators of the process of neurodegeneration at the level of the BBB may offer new avenues for novel therapeutic approaches for the treatment of numerous chronic neurodegenerative disorders currently without effective medication. KeywordsAging, blood-brain barrier, endothelial cells, neurodegenerative disorders, transport systems Structure of the blood-brain barrier (BBB)Homeostasis of the extracellular microenvironment in the neural tissue of the brain as well as its protection against neurotoxic compounds and variations in the composition of the blood are important for normal function of the neurons. It is warranted by a structure formed between blood and brain, which is therefore called the BBB.1 Clear evidence for the existence of this permeability barrier in the brain emerged in 1909 with the demonstration by Edwin Goldman (a South African-German) that a dye injected into the blood stream of a rat stained the whole body -except for the brain and spinal cord. The opposite was also true: injection of the dye into the cerebral ventricles stained the brain and spinal cord but not the rest of the body. 2,3 This occurs because most organs of the body are perfused by capillaries lined with endothelial cells (ECs) that have small pores (fenestrations) to allow for the rapid movement of small molecules into the organ interstitial fluid from the circulation. However, the capillary endothelium of the brain and spinal cord lack these pores because the ECs of brain capillary are connected to each other by continuous tight junctions (TJs), produced by the interaction of several transmembrane proteins that project into and seal the paracellular pathway.3-5 The interaction of these junctional proteins effectively blocks the free diffusion of

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Age-dependent changes in the structure, composition and biophysical properties of a human basement membrane

Cited by 163 publications

References 56 publications

An active role for basement membrane assembly and modification in tissue sculpting

An active role for basement membrane assembly and modification in tissue sculpting

A study of the dexamethasone sodium phosphate release properties from a periocular capsular drug delivery system

Age-associated physiological and pathological changes at the blood–brain barrier: A review

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