Age‐related alterations in superoxide anion generation in mouse peritoneal macrophages studied by repeated stimulations and heat shock treatment

Abstract

The ability of thioglycollate-elicited peritoneal macrophages (PM) from young and senescent mice to generate superoxide anions (O2-) under repeated stimulation or thermal stress was studied using either zymosan, opsonized zymosan (OZ), or phorbol myristate acetate (PMA). A diminished capacity to recover from repeated stimulation was found with aging. When stimulated for a second time 24 hours after the primary stimulation, PM from young animals generated 80% of the initial O2- responses to either zymosan, or OZ. Under the same conditions, PM from senescent mice generated 62% of the initial O2- produced in response to zymosan, and 45% in response to OZ. In both age groups the response to a second PMA stimulation comprised only 10% of the primary response. A considerably diminished capacity to generate O2- was also demonstrated in PM from senescent mice after recovery from exposure to thermal stress. Exposure to 42.5 degrees C for 20 minutes was found to be the threshold temperature for irreversible loss of activity in senescent PM, whereas at this temperature, PM from young animals recovered up to 70% of their O2- generating activity. Since NADPH oxidase and superoxide dismutase activities were only mildly affected by the hyperthermia in all age groups, they could not account for the age-related decline in the recovery from stress. Age-related alterations in signal transduction or receptor alterations could possibly play a primary role in this decline.

Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum improves phagocytic potential of macrophages in aged mice

“…A similar decline in extracellular and intracellular O 2 .-production was also observed in aged mice, relative to their young counterparts. These observations are concurrent with previous report (Lavie et al 1992). Contrary to peritoneal macrophages, the production of ROI by splenic macrophages increased in aged mice.…”

Age‐dependent changes in thymic macrophages and dendritic cells

“…Some authors have shown this capacity to be diminished or unchanged in aged subjects (De la Fuente, 1985;Ginaldi et al, 1999;Krause et al, 1999), while others have found an increased phagocytic capacity of macrophages during old age (De la Kay, 1996;Ortega et al, 1993Ortega et al, , 2000Wustrow et al, 1982). Similarly controversial are the results about the oxygen-dependent microbicide processes in old individuals (Alvarez et al, 1996;Alvarez and Santa Maria, 1996;De la Fuente, 1985Ding et al, 1994;Lavie et al, 1992;Ortega et al, 2000). Few studies have analyzed the antigen-presenting capacity of macrophages from aged individuals that seems to be reduced or unimpaired (De la Fuente, 1985;Perkins et al, 1982;Vetvicka et al, 1985).…”

In vitro age dependent response of macrophages to micro and nano titanium dioxide particles

“…Even though it has been demonstrated that macrophages from different species and different organs can respond differently to an array of stimuli (radiation, drugs, toxins, metal ions), few and controversial studies have addressed the influence of age on the function of macrophages. Studies performed in senescent individuals show an increase in mononuclear phagocyte system function, while others, report a decrease in immune system activity . It is noteworthy that today, metallic biomedical devices are not only employed to treat adult patients but are increasingly being used in the young.…”