Age-Related Effects of Orthovanadate Nanoparticles Involve Activation of GSH-Dependent Antioxidant System in Liver Mitochondria

Nikitchenko, Yuri V.; Klochkov, Vladimir K.; Kavok, Nataliya; Karpenko, N. A.; Yefimova, Svetlana; Nikitchenko, Irina V.; Божков, А. И.

doi:10.1007/s12011-020-02196-7

Cited by 15 publications

(10 citation statements)

References 54 publications

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“…Conclusions. Published earlier data on the beneficial effect of orthovanadate NPs during normal and accelerated ageing on physiological and biochemical indices of ageing organism [12,14,15] are consistent with those of the present work on the possibility of a correction of agerelated changes and an increase in the survival of ageing animals with NPs. Accounting for the survival curves and the stabilizing effect of the investigated compounds on the prooxidant/antioxidant balance, we can conclude that they are promising for the development of geroprotective drugs that slow down the ageing process.…”

Section: Kr C C Exp Exp T Exp T C C C C C C Exp T Exp T Exp T Exp T Esupporting

confidence: 92%

“…The parameters of the prooxidant/antioxidant balance were measured using the methods described by us earlier [4,5,12]. LHPO content in liver was determined by the method of Ohkawa et al (1979), and in serum -by the method of Asakawa et al (1980) spectrophoto metrially (UV VIS, Germany).…”

mentioning

confidence: 99%

“…Protein content was determined by the Lowry method in the modification by Miller (1959). The integral index of the prooxidant/antioxidant balance in liver mitochondria of young and old animals as reliability coefficient (Kr) was calculated using the formula [12]:…”

mentioning

confidence: 99%

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Cerium oxide nanoparticles and metformin increase the reliability of the prooxidant/antioxidant balance and the survival of ageing rats

Nikitchenko¹,

Klochkov²,

Kavok³

et al. 2020

Dopov. Nac. akad. nauk Ukr.

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In comparison with the action of metformin — mimetic of the calorie restricted diet, which prolong the animals lifespan, it has been studied the effect of nanoparticles of cerium dioxide (CeO2 NPs (1-2 nm)) on survival, physiological indices (the concentration of thyroxine, rectal temperature), as well as biochemical indices (content of lipid hydroperoxides, aconitase, glutathione peroxidase, glutaredoxin, glutathione reductase, NADP+ -dehydrogenase activities (glucose-6-phosphate-, malate- and isocitrate dehydrogenase)) in serum, liver mitochondrial and postmitochondrial fraction of ageing rats. It has been found that the prolonged use of CeO2 NPs with drinking water (0.25-0.30 mg/kg of body weight per day) and metformin (100-110 mg/kg of body weight per day) from 670 days after the birth lead to a significant increase in survival (the median survival of control rats was 900, in animals treated with CeO2 NPs — 960, and metformin — 990 days), a decrease in rectal temperature, a slowdown in the decrease in the level of thyroxine in blood, and normalization of the prooxidant/antioxidant balance in liver and blood tissues. Calculated integral index of reliability of prooxidant-antioxidant balance (reliability coefficient) in the studied tissues of rats in response to the application of the CeO2 NPs increased 4.99 times, and metformin — 4.94 times compared to intact animals. The data obtained allow us concluding about the prospects of the CeO2 NPs, as well as metformin for the development of geroprotector drugs, increasing the healthspan and survival of an ageing organism.

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Section: Kr C C Exp Exp T Exp T C C C C C C Exp T Exp T Exp T Exp T Esupporting

confidence: 92%

mentioning

confidence: 99%

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Cerium oxide nanoparticles and metformin increase the reliability of the prooxidant/antioxidant balance and the survival of ageing rats

Nikitchenko¹,

Klochkov²,

Kavok³

et al. 2020

Dopov. Nac. akad. nauk Ukr.

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“…Scarlata and O'Flaherty (2020) indicated the leading role of enzymes in the antioxidant defense system to maintain reproductive capacity. Nikitchenko et al (2021b) confirmed the effects of gadolinium orthovanadate NPs by activating the GSH-dependent antioxidant system in male rats. Barati et al (2020) also indicated the effectiveness of antioxidant therapy as a pathogenetic method in male infertility in humans, and drew attention to the effect of OS correction on sperm motility.…”

Section: Discussionsupporting

confidence: 55%

Effect of Gadolinium Orthovanadate Nanoparticles on Male Rabbits' Reproductive Performance under Oxidative Stress

Koshevoy¹,

Naumenko²,

Skliarov³

et al. 2022

WVJ

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Oxidative stress as a leading factor of male infertility requires correction with modern pharmacological agents, particularly redox-active nanoparticles, to improve sperm quality and hormonal balance. The current experimental study aimed to investigate the effect of orthovanadate nanoparticles of rare earth elements, particularly Gadolinium, with pronounced redox properties on the reproductive function of male rabbits under oxidative stress. A total of 36 mature male Hyla rabbits were divided into three groups of intact control (n = 12) and two experimental groups, including rabbits ubder oxidative stress (n = 12), induced by the introduction of tert-Butyl hydroperoxide, and those under oxidative stress plus hydrosol of gadolinium orthovanadate nanoparticles (NPs, n = 12) intake for 14 days. There were four rabbits per three replicates in each group. Animals of all groups were kept on the same diet and had free access to water. The use of NPs led to an improvement in sperm quality indicators. There was an improvement in motility and ejaculate volume indicators (by 14.6% and 39.2%, respectively), a reduction of the content of morphologically abnormal sperm by 26.7%; normalization of sex hormones, an increase in the level of total testosterone (by 113%) with a decrease in 17-β-estradiol (by 16.5%). This sex hormones improvement led to an increase in the androgen saturation of the rabbit’s body (free androgen index at the end of the experiment was 36.5%). The obtained changes were accompanied by a decrease in the oxidative load, as evidenced by a reduced content of diene conjugates and thio-barbituric acid-reactive compounds in the blood serum of rabbits by 30.4% and 26.8%, compared to the control. At the same time, there was an increase in the antioxidant potential, especially its glutathione link – the activity of glutathione peroxidase and glutathione reductase (by 42.5% and 34.2%, respectively), and the content of reduced glutathione increased by 62.3%, compared to the indicators before the introduction of NPs. The results of the study confirmed the effectiveness of using gadolinium orthovanadate NPs to correct the reproductive function of males under oxidative stress.

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“…OV NPs lowered the respiratory metabolic pathways and oxidative phosphorylation hence the ATP production in the isolated mitochondria from aged rats. Nikitchenko et al (2021b) established that continued use of OV NPs will increase the chance of survival and lifespan of the organism by stabilizing the redox balance. Agerelated macular degeneration causes blindness in old-age people due to oxidative damage.…”

Section: In Cell Longevitymentioning

confidence: 99%

Biomedical applications of metal oxide nanoparticles in aging and age-associated diseases

Yadav

Maurya

2021

3 Biotech

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Metal oxide nanoparticles are known to exhibit unique properties such as catalyzing the neutralization of superoxide anions, hydroxyl radicals, hydrogen peroxides and behave as antioxidant enzymes. Oxidative stress, damage and chronic inflammation are major causes and consequences of aging and age-associated disorders. With the increasing popularity of metal oxide nanoparticles, they have been applied in various age-related pathologies using their antioxidant property. Metal oxide nanoparticles have been used as diagnostic, therapeutic, and as theranostics. This review summarizes the applications of metal oxide nanoparticles in aging and age-associated disorders such as cardiovascular diseases, diabetes, cancer, neurodegenerative disorders. Oxidative stress plays a central role in the activation of inflammatory pathways, disturbing the mitochondrial function, decreasing the telomere length and leading the cell towards senescence or death. Oxidative damage is the common pathway in the progression of aging and related diseases. Metal oxide nanoparticles scavenge or precisely detect the generated reactive oxygen species, hence applied in both diagnostics and therapeutics.

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Age-Related Effects of Orthovanadate Nanoparticles Involve Activation of GSH-Dependent Antioxidant System in Liver Mitochondria

Cited by 15 publications

References 54 publications

Cerium oxide nanoparticles and metformin increase the reliability of the prooxidant/antioxidant balance and the survival of ageing rats

Cerium oxide nanoparticles and metformin increase the reliability of the prooxidant/antioxidant balance and the survival of ageing rats

Effect of Gadolinium Orthovanadate Nanoparticles on Male Rabbits' Reproductive Performance under Oxidative Stress

Biomedical applications of metal oxide nanoparticles in aging and age-associated diseases

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