Age-related subproteomic analysis of mouse liver and kidney peroxisomes

Abstract

Background: Despite major recent advances in the understanding of peroxisomal functions and how peroxisomes arise, only scant information is available regarding this organelle in cellular aging. The aim of this study was to characterize the changes in the protein expression profile of aged versus young liver and kidney peroxisome-enriched fractions from mouse and to suggest possible mechanisms underlying peroxisomal aging. Peroxisome-enriched fractions from 10 weeks, 18 months and 24 months C57bl/6J mice were analyzed by quantitative proteomics.

Proteome analysis in the assessment of ageing

“…The expression and the activity of the antioxidant enzyme catalase decreased significantly in older mice might result in an impairment of the detoxification of hydrogen peroxide, an metabolic by-product of peroxisomal fatty acid ␤-oxidation (Mi et al, 2007).…”

“…Kidney ageing was further investigated in the peroxisomes of male C57BL/6J mice at 18 and 24 months, using 2D PAGE combined with MALDI-TOF MS (Mi et al, 2007). The expression and the activity of the antioxidant enzyme catalase decreased significantly in older mice might result in an impairment of the detoxification of hydrogen peroxide, an metabolic by-product of peroxisomal fatty acid ␤-oxidation (Mi et al, 2007).…”

Protein targets for carbonylation by 4-hydroxy-2-nonenal in rat liver mitochondria

“…Increased levels of carbonylation and circulatory release of Cps1 have been shown to be directly associated with mitochondrial damage and/or impaired mitochondrial function in the liver during sepsis and upon alcohol-induced liver damage [16,48]. Several other enzymes identified here as targets of HNE-modification in the liver (Table 1), such as Acyl-CoA dehydrogenase, 3-ketoacyl-CoA, catalase, thiolase, glutamate dehydrogenase, etc., have also been specifically implicated in animal models of aging, alcoholism and metabolic diseases [13, 31,49–54]. …”

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

“…The components of functional mitochondria is encoded primarily by nuclear genes; mtDNA includes only 13 polypeptide genes, all of which encode essential components of oxidative phosphorylation (in addition to tRNA and rRNA genes [reviewed in 7]). Using 2-D PAGE in rodent liver tissue, the mitochondrial protein concentration varied in an age-dependent manner [27,28]. The majority of mitochondrial proteins that changed significantly with age (6-7 months vs. 24-25 months) were found in liver tissue [27].…”