Agglutination of Intravenously Administered Phosphatidylcholine‐Containing Lipid Emulsions With Serum C‐reactive Protein

Tugirimana, Pierrot Lundimu; Speeckaert, Marijn M.; Fiers, Tom; Buyzere, Marc L. De; Kint, J.; Benoît, Dominique; Delanghe, Joris

doi:10.1177/0884533612474040

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…However, SNPs in the FADS2 gene have been associated with circulating phospholipid trans fatty acid and plasma phospholipid n-3 fatty acid levels by the CHARGE Consortium [ 39 – 41 ]. CRP has been shown to bind phospholipids through phosphorylcholine [ 42 ] and plasma CRP values have been reported to drop with phospholipid-induced agglutination [ 43 ]. FADS2 has a known function in the synthesis of arachidonic acid that is relevant in inflammatory processes and has been associated with both CRP and risk of CVD [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

C-reactive protein upregulates the whole blood expression of CD59 - an integrative analysis

et al. 2017

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Elevated C-reactive protein (CRP) concentrations in the blood are associated with acute and chronic infections and inflammation. Nevertheless, the functional role of increased CRP in multiple bacterial and viral infections as well as in chronic inflammatory diseases remains unclear. Here, we studied the relationship between CRP and gene expression levels in the blood in 491 individuals from the Estonian Biobank cohort, to elucidate the role of CRP in these inflammatory mechanisms. As a result, we identified a set of 1,614 genes associated with changes in CRP levels with a high proportion of interferon-stimulated genes. Further, we performed likelihood-based causality model selection and Mendelian randomization analysis to discover causal links between CRP and the expression of CRP-associated genes. Strikingly, our computational analysis and cell culture stimulation assays revealed increased CRP levels to drive the expression of complement regulatory protein CD59, suggesting CRP to have a critical role in protecting blood cells from the adverse effects of the immune defence system. Our results show the benefit of integrative analysis approaches in hypothesis-free uncovering of causal relationships between traits.

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Section: Resultsmentioning

confidence: 99%

C-reactive protein upregulates the whole blood expression of CD59 - an integrative analysis

et al. 2017

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“…Both in vitro and in vivo, CRP has been reported to produce agglutination ('creaming') of Intralipid ® (Fresenius Kabi, Bad Homburg vor der Höhe, Germany), an intravenously administered lipid emulsion [9][10][11]. Since CRP can bind phospholipids [8,9,12], we studied the interaction between CRP and two commercial lipid-containing AmB formulations (L-AmB and ABLC) both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

C-reactive protein interacts with amphotericin B liposomes and its potential clinical consequences

Delanghe

Himpe

Boelens

et al. 2023

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives Amphotericin B (AmB) is the gold standard for treating invasive fungal infections. New liposomal-containing AmB formulations have been developed to improve efficacy and tolerability. Serum/plasma C-reactive protein (CRP) values are widely used for monitoring infections and inflammation. CRP shows a high affinity to phosphocholine and it aggregates structures bearing this ligand, e.g. phosphocholine-containing liposomes. Therefore, we studied the interaction between CRP and phosphocholine-containing liposomal AmB preparations in vivo and in vitro. Methods CRP was prepared by affinity chromatography. Liposomal AmB (L-AmB, AmBisome®) was spiked (final concentrations of L-AmB: 150 mg/L) to CRP-containing serum (final CRP concentration: 300 mg/L). Following the addition of L-AmB, complex formation was monitored turbidimetrically. The size of CRP-L-AmB complexes was assessed using gel filtration. CRP was monitored in patients receiving either L-Amb or AmB lipid complex (ABLC). Results Following addition of L-AmB to CRP-containing plasma, turbidimetry showed an increase in absorbance. These results were confirmed by gel permeation chromatography. Similarly, in vivo effects were observed following intravenous administration of AmBisome®: a decline in CRP values was observed. In patients receiving L-Amb, decline of CRP concentration was faster than in patients receiving ABLC. Conclusions In vitro experiments are suggestive of a complexation between CRP and liposomes in plasma. Interpretation of CRP values following administration of AmBisome® might be impaired due to this complexation. In vivo formation of complexes between liposomes and CRP might contribute, or even lead, to intravascular microembolisation. Similar effects have been described following the administration of Intralipid® and other phosphocholine-containing liposomes.

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Characteristics of apparently healthy individuals with a very low C-reactive protein

Ziv‐Baran

Wasserman

Goldiner

et al. 2019

Clinica Chimica Acta

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Agglutination of Intravenously Administered Phosphatidylcholine‐Containing Lipid Emulsions With Serum C‐reactive Protein

Abstract: In this in vitro experiment, we demonstrated agglutination of CRP with phospholipids in various fat emulsions. Research studies are required in patients to determine which effects occur with various intravenous fat emulsions.

Cited by 3 publications

References 26 publications

C-reactive protein upregulates the whole blood expression of CD59 - an integrative analysis

C-reactive protein upregulates the whole blood expression of CD59 - an integrative analysis

C-reactive protein interacts with amphotericin B liposomes and its potential clinical consequences

Characteristics of apparently healthy individuals with a very low C-reactive protein

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