Aggressiveness variation of Fusarium graminearum isolates from Argentina following point inoculation of field grown wheat spikes

Malbrán, Ismael; Mourelos, Cecilia Alejandra; Girotti, Juan Roberto; Aulicino, Mónica Beatriz; Balatti, Pedro Alberto; Lori, Gladys Albina

doi:10.1016/j.cropro.2012.05.025

Cited by 42 publications

(24 citation statements)

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“…Then, the necrotrophic phase is developed with intracellular hyphae growth in the host and beginning of mycotoxin production [35]. Even though, the initial infection or establishment of infection depends on the inoculum level, environmental conditions, and the state of development of the host; it is appropriate to consider also the aggressiveness variation among isolates as Malbrán et al [3] suggested.…”

Section: Discussionmentioning

confidence: 99%

“…Fusarium head blight (FHB) is one of the most devastating diseases of small-grain cereals. Severe epidemics have occurred all over the world, affecting wheat in all cropping areas around the world, including those in Argentina, altering the yield and quality of grains, as manifest in their weight, carbohydrate and protein composition, and the mycotoxins presence such as deoxynivalenol (DON) [1][2][3]. Fusarium graminearum is the main etiologic agent of this disease in South America.…”

Section: Introductionmentioning

confidence: 99%

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Screening ofFusarium graminearumIsolates for Enzymes Extracellular and Deoxynivalenol Production

Ortega

Kikot

Astoreca

et al. 2013

Journal of Mycology

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Fusarium graminearum, the main etiological agent of Fusarium head bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present paper, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic, and lipase extracellular enzymatic activities production, as well as for the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity after advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation to the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research would allow us to apply a selection criterion among isolates for further research.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Screening ofFusarium graminearumIsolates for Enzymes Extracellular and Deoxynivalenol Production

Ortega

Kikot

Astoreca

et al. 2013

Journal of Mycology

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“…However, spray inoculation covers both Type I and Type II resistances, while inoculation of spores in a single floret per head (point inoculation) estimates Type II resistance [6,7]. Point inoculation in FHB evaluation is a relatively stable method and is less affected by environment in terms of disease development [8]. [5] illustrated that some wheat lines could have both Type I and Type II resistance present while others could have only one type of resistance.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Spray and Point Inoculation with <i>Fusarium graminearum</i> to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress

Okereke¹

2017

CBB

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Two near isogenic lines of wheat; Mercia 1 (Rht-B1b) and Mercia 2 (Rht-D1b) were evaluated for Type I and Type II resistance using point or spray inoculation and incubated under controlled environment. The experiment was a complete factorial combination of 2 x 4 x 2 x 4 {2 genotypes, 4 inoculation treatments (spray + Fusarium, spray + SDW, point + Fusarium, point + SDW) x 2 temperatures} and 4 randomised replicates. Wheat spikes were sprayed with a single spore isolate of F. graminearum 4 days after the start of flowering and transferred to controlled environment cabinets set at either 23/15°C or 28/20°C for 14 days and then taken outside to mature. Results reveals that genotype showed no difference in FHB severity in both spray inoculation and point inoculation but the temperature main effect only approached significance (P=0.071) with low temperature increasing FHB severity following point inoculation. High temperature significantly (P<0.001) increased DON concentration in spray inoculation, contrasted the effect in point inoculation. However, the amount of DON per grain showed no significant (P>0.05) effect. Grains per spike was significantly (P<0.011) reduced by 25% following spray when compared with point inoculation, but showed no significant (P>0.05) effect of temperature. On average, Fusarium infection significantly (P<0.001) reduced wheat grain weight by 28% when compared with uninoculated control.

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“…Sm.) (Enfermedades…, 2004), y F. graminearum es la especie predominante en la zona central de Argentina (Malbrán et al, 2012). Fusarium graminearum se ha aislado también de raíces, tallos, frutos, y semillas de otros cultivos de la familia Fabaceae, tales como soja, lentejas, habas y arvejas (Pioli et al, 2000).…”

Section: Introductionunclassified

Micotoxinas en harinas derivadas de trigo y soja detectadas por prueba de Elisa

Peruzzo

Pioli²

2016

Pesq. agropec. bras.

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Resumen -El objetivo de este trabajo fue evaluar la presencia de micotoxinas en las harinas derivadas de trigo y soja expuestos a la infección de Fusarium graminearum y detectadas por prueba de Elisa. La contaminación fue evaluada durante 2010 y 2012, en condiciones de invernadero, a través de inoculaciones artificiales, y en lotes de producción en campo, expuestos a infecciones naturales. Se utilizaron 26 muestras de harinas derivadas de cariopsis de trigo y semillas de soja. La detección de micotoxinas deoxinivalenol y zearalenona se realizó por kit de Elisa. En harinas de trigo de diversos ambientes, se detectaron ambas micotoxinas -deoxinivalenol y zearalenona -, mientras que en harinas de soja sólo se detectó zearalenona. Las concentraciones de esas micotoxinas observadas en las harinas no son admisibles para el consumo humano y animal. El kit de Elisa constituyó una herramienta biotecnológica efectiva para la detección de la contaminación predominante de zearalenona, producida por F. graminearum, en harinas de trigo y soja de diferentes ambientes semicontrolados y naturales.Términos para indexación: Fusarium graminearum, Glycine max, Triticum aestivum, deoxinivalenol, zearalenona. Mycotoxins in flours derived from wheat and soybean detected by the Elisa testAbstract -The objective of this work was to evaluate the presence of mycotoxins in flours derived from wheat caryopses and soybean seed exposed to Fusarium graminearum infection and detected by the Elisa test. Contamination was evaluated during 2010 and 2012, in greenhouse conditions, by artificial inoculation, and on field-production batches exposed to natural infections. Twenty-six samples of flours derived from wheat caryopses and soybean seeds were used. Detection of the mycotoxins deoxynivalenol and zearalenone was performed by an Elisa kit. In wheat flour of different environments both mycotoxins -deoxynivalenol and zearalenone -were detected, while in soybean flour only zearalenone was detected. Mycotoxins concentrations observed in these flours are inappropriate for human and animal consumption. Elisa kit constituted an effective biotechnological tool for the detection of prevailing contamination by zearalenone, caused by F. graminearum in wheat and soybean flours of different, semicontrolled and natural environments.

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Aggressiveness variation of Fusarium graminearum isolates from Argentina following point inoculation of field grown wheat spikes

Cited by 42 publications

References 48 publications

Screening ofFusarium graminearumIsolates for Enzymes Extracellular and Deoxynivalenol Production

Screening ofFusarium graminearumIsolates for Enzymes Extracellular and Deoxynivalenol Production

Comparison of Spray and Point Inoculation with <i>Fusarium graminearum</i> to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress

Micotoxinas en harinas derivadas de trigo y soja detectadas por prueba de Elisa

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Aggressiveness variation of Fusarium graminearum isolates from Argentina following point inoculation of field grown wheat spikes

Cited by 42 publications

References 48 publications

Screening ofFusarium graminearumIsolates for Enzymes Extracellular and Deoxynivalenol Production

Screening ofFusarium graminearumIsolates for Enzymes Extracellular and Deoxynivalenol Production

Comparison of Spray and Point Inoculation with &lt;i&gt;Fusarium graminearum&lt;/i&gt; to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress

Micotoxinas en harinas derivadas de trigo y soja detectadas por prueba de Elisa

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Comparison of Spray and Point Inoculation with <i>Fusarium graminearum</i> to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress