Aging of In Vitro Pulp Illustrates Change of Inflammation and Dentinogenesis

Lee, Younghee; Kim, Go-Eun; Cho, Hyejin; Yu, M.K.; Bhattarai, Govinda; Lee, Nan-Hee; Yi, Ho-Keun

doi:10.1016/j.joen.2012.10.031

Cited by 50 publications

(46 citation statements)

References 33 publications

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“…Throughout life odontoblasts continue to secrete an extracellular matrix that becomes mineralized and as a consequence the pulp chamber narrows with age . The molecular regulation of this process is unknown.…”

Section: Discussionmentioning

confidence: 99%

Wnt Signaling Regulates Pulp Volume and Dentin Thickness

Lim

Liu

Cheng

et al. 2013

Journal of Bone and Mineral Research

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Odontoblasts, cementoblasts, ameloblasts and osteoblasts all form mineralized tissues in the craniofacial complex, and all these cell types exhibit active Wnt signaling during postnatal life. We set out to understand the functions of this Wnt signaling, by evaluating the phenotypes of mice in which the essential Wnt chaperone protein, Wingless was eliminated. The deletion of Wls was restricted to cells expressing Osteocalcin, which in addition to osteoblasts includes odontoblasts, cementoblasts, and ameloblasts. Dentin, cementum, enamel, and bone all formed in OCN-Cre;Wlsfl/fl mice but their homeostasis was dramatically affected. The most notable feature was a significant increase in dentin volume and density. We attribute this gain in dentin volume to a Wnt-mediated mis-regulation of Runx2. Normally, Wnt signaling stimulates Runx2, which in turn inhibits DSP; this inhibition must be relieved for odontoblasts to differentiate. In OCN-Cre;Wlsfl/fl mice, Wnt pathway activation is reduced and Runx2 levels decline. The Runx2-mediated repression of DSP is relieved and odontoblast differentiation is accordingly enhanced. This study demonstrates the importance of Wnt signaling in the homeostasis of mineralized tissues of the craniofacial complex.

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Section: Discussionmentioning

confidence: 99%

Wnt Signaling Regulates Pulp Volume and Dentin Thickness

Lim

Liu

Cheng

et al. 2013

Journal of Bone and Mineral Research

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“…, Lee et al . ), which stimulate the nerve fibres of the periodontal ligament and the pulp releasing neuropeptides to regulate all the vascular and cellular processes that occur in the premature pulp ageing forming tertiary reparative dentine as a response to harmful stimuli (Rodd & Boissonade , Caviedes‐Bucheli et al . , Lee et al .…”

Section: Literature Reviewmentioning

confidence: 99%

“…A singular characteristic of pulp microvasculature is the presence of arteriovenous anastomoses (AVA), which participate in the regional control of the blood flow due to its dilation or contraction in the presence of thermal, chemical or mechanical irritation (Matthews & Andrew 1995, Heyeraas & Berggreen 1999, Rodd & Boissonade 2002, such as occlusal trauma, where an alteration in the vasculature takes place, thereby increasing vascular permeability and vascular stasis and decreasing the resistance of the vessels and causing oedema. All these changes are caused not only by the local control mechanisms of the blood vessels (presence of pre-and post-capillary sphincters) but also by the main control performed by the nervous system in the regulation of pulp blood flow due to the release of neurotransmitters by the sympathetic, parasympathetic and somatosensory fibres such as SP (Kim 1990, Olgart 1996, Rodd & Boissonade 2003, Caviedes-Bucheli et al 2008, Lee et al 2013.…”

Section: Dental Pulp Microcirculation and Innervationmentioning

confidence: 99%

“…The pulp suffers physiological modifications throughout its life due to phenomena such as mastication. It also reacts in the presence of pathological situations such as caries, cavity preparation, dental materials and occlusal trauma (Tziafas 1994, von Bohl et al 2012, Lee et al 2013, which stimulate the nerve fibres of the periodontal ligament and the pulp releasing neuropeptides to regulate all the vascular and cellular processes that occur in the premature pulp ageing forming tertiary reparative dentine as a response to harmful stimuli (Rodd & Boissonade 2003, Caviedes-Bucheli et al 2011a, Lee et al 2013.…”

Section: Occlusal Trauma and Neurogenic Inflammationmentioning

confidence: 99%

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Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma

et al. 2016

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“…DMP-1 and DSPP are known as mineralization markers in the odontoblasts-like differentiation of HDPCs. DMP-1 is essential to the formation and mineralization of dentine (22), while DSPP is the predominant non-collagen protein in dentin formation (23,24). These results demonstrated a tight coupling between TRPC6 and odontoblastic differentiation, which may be mediated by calcium signaling.…”

Section: Discussionmentioning

confidence: 99%

Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells

Yang

Song

Wang

et al. 2017

Experimental and Therapeutic Medicine

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Pulp capping is a restorative technique employed in an attempt to maintain pulpal vitality and generate reparative dentin. Ca2+ released from capping materials is suggested to promote reparative dentin formation. Transient receptor potential channel 6 (TRPC6) is a receptor-operated Ca2+ channel that serves an important role in Ca2+ influx in the majority of non-excitable cells, and influences the calcium signaling and cell respond. Therefore, the purpose of the present study was to gain an insight into the role of TRPC6 in the odontoblastic differentiation of human dental pulp cells (HDPCs). Human dental pulp tissues and HDPCs were obtained from healthy third molars. By immunohistochemical staining, TRPC6 was observed to be highly expressed in the dental pulp tissue, particularly in the odontoblast layer. In addition, the protein level of TRPC6 was increased in a time-dependent manner during odontogenic differentiation of HDPCs. Downregulation of TRPC6 by a lentivirus vector containing TRPC6 shRNA inhibited the process of odontogenic differentiation in HDPCs. In conclusion, the current data demonstrated that TRPC6 served a significant role in the odontogenic differentiation of HDPCs, suggesting it may be a promising therapeutic target in regenerative endodontics.

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Aging of In Vitro Pulp Illustrates Change of Inflammation and Dentinogenesis

Cited by 50 publications

References 33 publications

Wnt Signaling Regulates Pulp Volume and Dentin Thickness

Wnt Signaling Regulates Pulp Volume and Dentin Thickness

Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma

Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells

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