Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas

Abstract

The taxonomic position of a lemon-yellow-pigmented actinobacterium, strain K22-21 T , isolated from a soil sample from Lahaul-Spiti Valley in the Indian Himalayas, was determined using a polyphasic approach. The strain had phenotypic and chemical properties that were consistent with its classification in the genus Agrococcus. Alignment of the 16S rRNA gene sequence of strain K22-21 T with sequences from Agrococcus jenensis DSM 9580 T , Agrococcus baldri DSM 14215 T and Agrococcus citreus DSM 12453 T revealed similarities of 98?5, 96?8 and 96?6 %, respectively. However, the level of DNA-DNA relatedness between strain K22-21 T and A. jenensis was 55?1 %. The novel strain could be distinguished from type strains of the three species of the genus Agrococcus using DNA-DNA relatedness and phenotypic data. Based on these differences, strain K22-21 T (=MTCC 7154 T =DSM 17612 T ) should be classified as the type strain of a novel species of Agrococcus, for which the name Agrococcus lahaulensis sp. nov. is proposed.

Stress-Tolerance and Taxonomy of Culturable Bacterial Communities Isolated from a Central Mojave Desert Soil Sample

“…The bacteria of genera Arthrobacter, Bacillus, Kocuria, Leucobacter, Massilia, Mycetocola, Microbacterium, Paracoccus, Planomicrobium, Pseudarthrobacter, Rufibacter, Sphingomonas, and Streptomyces were previously isolated from other hot deserts [44,[63][64][65]. Bacteria of the genus Agrococcus were previously cultured from cold deserts [66]. Among the representatives of the genus Brachybacterium several species connected to the root systems of halophilic plants were described and characterized by having plant growth-promoting potential [67].…”

“…The highest levels of resistance to physicochemical stress factors were found in representatives of the genera Arthrobacter, Bacillus, Kocuria, Leucobacter, Microbacterium, Planomicrobium, and Pontibacter (Table S1). Representatives of these genera are widely distributed in desert soils and have previously demonstrated high resistance to various types of stress effects [15,17,22,25,44,59,66].…”

Agrococcus terreus sp. nov. and Micrococcus terreus sp. nov., isolated from forest soil

“…A. jejuensis SSW1-48 T ; 3, A. baldri V-108 T ; 4, A. jenensis 2002-39/1 T ; 5, A. citreus D-1/1a T ; 6, A. versicolor K114/01 T ; 7, A. lahaulensis K22-21 T ; 8, A. casei R-17892t2 T . Data were taken from Groth et al (1996), Wieser et al (1999), Zlamala et al (2002), Mayilraj et al (2006), Bora et al (2007), Behrendt et al (2008), Lee (2008) and this study. A. terreus DNG5 T , A. jejuensis DSM 22002 T , A. baldri JCM 12132 T , A. jenensis JCM 9950 T and A. citreus JCM 12398 T were tested in parallel with the API 20NE system in this study.…”

“…html). Members of the genus Agrococcus have been isolated from various environments including soil samples (Groth et al, 1996;Wieser et al, 1999;Mayilraj et al, 2006), cheese surfaces (Bora et al, 2007), potato plants (Behrendt et al, 2008) and dried seaweed (Lee, 2008), as well as from the air (Zlamala et al, 2002). Chemotaxonomically, species of Agrococcus contain 2,4-diaminobutyric acid as the characteristic cell-wall diamino acid, phosphatidylglycerol and diphosphatidylglycerol as the major lipids (polar lipids have not been determined for Agrococcus versicolor), anteiso-C 15 : 0 as the major fatty acid and acetyl-type murein.…”

First Draft Genome Sequence from a Member of the Genus Agrococcus , Isolated from Modern Microbialites

“…The genus Agrococcus is classified within the family Microbacteriaceae , members of which exhibit the unusual feature of having diaminobutyric acid within the cell wall, which imparts a distinctive yellow color (1). The genus has eight described species that have been isolated from a wide range of environments, including air (2), a coal mine (3), cheese (4), cold desert soil (5), forest soil (6), a medieval wall painting (7), dried seaweed (8), and the phyllosphere of potato plants (9). The genetic richness and diversity of this genus is poorly known, with little genetic information available other than for the 16S rRNA genes.…”