AIDS-associated Talaromyces marneffei central nervous system infection in patients of southwestern China

Li, Yuye; Dong, Rong‐Jing; Shrestha, Samip; Upadhyay, Pratishtha; Li, Huiqin; Kuang, Yi‐Qun; Yang, Xiuwen; Zhang, Yungui

doi:10.1186/s12981-020-00281-4

Cited by 14 publications

(10 citation statements)

References 24 publications

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“…The reduction of CD4 + T cells is a common phenomenon in HIV or non-HIV patients with T. marneffei infection. 12 The binding of B7 molecules to different ligands results in the difference of Ca 2+ influx, thus affecting the activation state of T cells. The interaction between CD86 and CD28 provides the signals needed to trigger the activation, expansion and differentiation of T cells, which increased Th1 cells differentiation.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Talaromyces marneffei Infection on CD86 Expression in THP-1 Cells

Yang¹,

Shen²,

Chen³

et al. 2021

IDR

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Background Talaromyces marneffei ( T. marneffei ) is a destructive opportunistic dimorphic fungal which can cause lethiferous Talaromycosis, but the clearance of T. marneffei mainly depends on the innate immune response. Objective To investigate whether T. marneffei can inhibit the expression of CD86 in THP-1 cells after infection and discuss the potential mechanisms. Methods Western blot and immunoelectron microscopy were used to detect the CD86 expression on T. marneffei cultured on BHI medium at 37°C. Western blot, enzyme-linked immunoassay and immunofluorescence were used to detect the change of CD86 expression on macrophages incubating with T. marneffei . Enzyme-linked immunoassay was used to detect the content of CD86 in supernatant in the co-culture system. Immunohistochemistry and immunoelectron microscopy were used to detect the expression of CD86 on T. marneffei incubating with macrophages. Results T. marneffei did not express CD86 when cultured separately at 37°C detected by Western blot and immunoelectron microscopy, but it did express CD86 when incubated with macrophages detected by immunohistochemistry and immunoelectron microscopy. The CD86 expression of macrophages significantly decreased at 72 hours when infected with T. marneffei while the content of CD86 in supernatant significantly increased at 72 hours compared with the control group which were detected by Western blot, enzyme-linked immunoassay and immunofluorescence. Conclusion 1) After T. marneffei infection, CD86 expression on THP-1 decreased, and with the progression of infection, insufficient polarization of M1 macrophages gradually appeared; 2) T. marneffei may adsorb or uptake CD86 in supernatant produced by macrophages during the contact with THP-1 cells, thus leading to the consumption of CD86 in macrophages.

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Section: Discussionmentioning

confidence: 99%

The Effect of Talaromyces marneffei Infection on CD86 Expression in THP-1 Cells

Yang¹,

Shen²,

Chen³

et al. 2021

IDR

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“…T cells mainly undertake cellular immune function and CD4+T cells are stimulated to differentiate into Th1 cells or Th2 cells.The reduction of CD4 + T cells is a common phenomenon in HIV or non-HIV patients with T.marneffei infection [12] . The binding of B7 molecules to different ligands results in the difference of Ca 2+ in ux, thus affecting the activation state of T cells.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Talaromyces Marneffei Infection on CD86 Expression in THP-1 Cells

Yang

Lin

Chen

et al. 2020

Preprint

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Background: Talaromyces Marneffei (T.marneffei) is an destructive opportunistic dimorphic fungal which can cause lethiferous Talaromycosis, but the clearance of T.marneffei mainly depends on the innate immune response. Objectives: To investigate the effect of T.marneffei on CD86 expression in THP-1 cells after infection and discuss the potential mechanisms. Methods: Western blot and immunoelectron microscopy were used to detect the CD86 expression on T.marneffei cultured on BHI medium at 37℃. Western blot、enzyme-linked immunoassay and immunofluorescence were used to detect the change of CD86 expression on macrophages incubating with T.marneffei. Enzyme-linked immunoassay was used to detect the content of CD86 in supernatant in the co-culture system. Immunohistochemistry and immunoelectron microscopy were used to detect the expression of CD86 on T.marneffei incubating with macrophages. Results: T.marneffei didn’t express CD86 when cultured separately at 37℃ detected by western blot and immunoelectron microscopy, but it did express CD86 when incubated with macrophages detected by immunohistochemistry and immunoelectron microscopy. The CD86 expression of macrophages significantly decreased at 72 hours when infected with T.marneffei while the content of CD86 in supernatant significantly increased at 72 hours compared with the control group which were detected by western blot, enzyme-linked immunoassay and immunofluorescence. Conclusion: 1.After T.marneffei infection，CD86 expression on THP-1 decreased，and with the progression of infection, insufficient polarization of M1 macrophages gradually appeared；2.T.marneffei may adsorb or uptake CD86 in supernatant produced by macrophages during the contact with THP-1 cells, thus leading to the consumption of CD86 in macrophages.

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“…When we tested the ICT strip with talaromycosis serum, the diagnostic sensitivity was reduced to 71.43% (15/21). In the future, we may have the opportunity to investigate the efficacy of the ICT system with other body fluids, such as bronchoalveolar lavage or cerebral spinal fluid [ 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin

et al. 2021

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Talaromyces marneffei is a thermally dimorphic fungus that causes opportunistic systemic mycoses in patients with AIDS or other immunodeficiency syndromes. The purpose of this study was to develop an immunochromatographic strip test (ICT) based on a solid phase sandwich format immunoassay for the detection of T. marneffei antigens in clinical urine specimens. The T. marneffei yeast phase specific monoclonal antibody 4D1 (MAb4D1) conjugated with colloidal gold nanoparticle was used as a specific signal reporter. Galanthus nivalis Agglutinin (GNA) was adsorbed onto nitrocellulose membrane to serve as the test line. Similarly, a control line was created above the test line by immobilization of rabbit anti-mouse IgG. The immobilized GNA served as capturing molecule and as non-immune mediated anti-terminal mannose of T. marneffei antigenic mannoprotein. The MAb4D1–GNA based ICT showed specific binding activity with yeast phase antigen of T. marneffei, and it did not react with other common pathogenic fungal antigens. The limit of detection of this ICT for T. marneffei antigen spiked in normal urine was approximately 0.6 μg/ml. The diagnostic performance of the ICT was validated using 341 urine samples from patents with culture- confirmed T. marneffei infection and from a control group of healthy individuals and patients with other infections in an endemic area. The ICT exhibited 89.47% sensitivity, 100% specificity, and 97.65% accuracy. Our results demonstrate that the urine-based GNA–MAb4D1 based ICT produces a visual result within 30 minutes and that the test is highly specific for the diagnosis of T. marneffei infection. The findings validate the deployment of the ICT for clinical use.

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AIDS-associated Talaromyces marneffei central nervous system infection in patients of southwestern China

Cited by 14 publications

References 24 publications

The Effect of Talaromyces marneffei Infection on CD86 Expression in THP-1 Cells

The Effect of Talaromyces marneffei Infection on CD86 Expression in THP-1 Cells

The Effect of Talaromyces Marneffei Infection on CD86 Expression in THP-1 Cells

An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin

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