Aislamiento de especies de Cryptococcus asociadas con Eucalyptus en un parque de Bogotá

Abstract

Con base en la información consignada en el herbario del Museo de Historia Natural de la Universidad Nacional sobre la presencia de un Eucalyptus camaldulensis en un parque de Bogotá. se diseñó este estudio con el fin de establecer si existia alguna asociación entre los eucaliptos presentes en el parque y las especies de ~ryptococcus. Se recolectaron mensualmente durante 2 años. muestras de madera de 19 Eucalv~tus. Estas se extraieron , .con una solución tampón fosfato con antibióticos y el extracto se sembró en medios selectivos. De las 426 muestras obtenidas. se recuperaron 17 aislamientos de especies de Cryptococcus: 14 C. laurentii. 2 C. neoformansvar neoformans y 1 C. albidus. Se estableció la asociación del hongo con los eucaliptos; sin embargo, no se logró recuperar la variedad gattii.Palabras clave: Cryptococcus neoformans, ecologia, Eucalyptus. lsolation of Cryptococcus species from Eucalyptus trees located in a park in BogotáThe presence in a northeast park of Bogota of a tree classified as Eucalyptus camaldulensis according to the registers of the National University herbarium prompted this study, aimed at establishing if there was an association of the Eucalyptus trees in this park with C~yptococcus spp. For 24 months. bark samples were monthly collected from 19 Eucalyptus trees. The samples were extracted with PBS buffer and cultured in a selective media. From 426 bark samples collected, 17 Cryptococcus isolates were recovered: 14 C. laurentii, 2 C. neoformans var neoformans serotype A and one C. albidus. The association between some Crypfococcus s~ecies and Eucalv~tus trees was aaain established: nevertheless. C. neoformans var. oaftii . .was not recovered.

Environmental Status of Cryptococcus neoformans and Cryptococcus gattii in Colombia

“…neoformans with two positive isolates, and finally one C. albidus isolate. Contrary to what is reported in the literature, where a close association between C. gattii and E. camaldulensis is described, in this study no isolates of this species were obtained [20].…”

“…Other isolates of C. neoformans have been reported in temperate and tropical climates, associated mainly with avian excreta [18,22,23,56,82]. Environmental isolates of these pathogenic species have been reported in different cities in Colombia, such as Popayán [18], Pasto [23], Cúcuta [26,27], Monteria [19], Bogotá [18,19], and municipalities of Cundinamarca [21,29,61], with Bogotá being the city with the highest number of positive samples [20,81]. These results are discussed throughout the article and are synthesized in Figure 1 and Table 1, where the record of environmental isolates of C. neoformans and C. gattii in different parts of Colombia are shown.…”

[RETRACTED ARTICLE]: Distribution and association between environmental and clinical isolates of Cryptococcus neoformans in Bogotá-Colombia, 2012-2015

“…In Colombia, environmental studies have reported isolates of C. neoformans var. grubii (serotype A) and C. gattii (serotype B and serotype C) from plant debris in trees like Eucalyptus sp , T. catappa , Corymbias and Ficus , and described that serotypes A and B were the most prevalent ( Castañeda & Castañeda 2001 , Granados & Castañeda 2005 , Quintero et al 2005 , Escandón et al 2010) . In Bogotá, studies reported C. neoformans and C. gattii from 192 environmental and clinical samples, being serotype A the most prevalent, followed by serotype B and C ( Ordoñez & Castañeda 1994) .…”

“…In 2001, Castañeda and Castañeda (2001) described the association between Cryptococcus and Eucalyptus from a local park in Bogotá, with a positivity of 4% during a two-year sampling period. In 2005, Granados and Castañeda (2005) collected 480 environmental samples with a positivity of 7.9% for C. neoformans and, in 2010, Escandón et al (2010) found a positivity of 11.7% for C. gattii isolated from Corymbia ficifolia in Bogotá.…”

Fatal fungaemia due to Cryptococcus albidus in an elderly diabetic woman presenting with pleural effusion

“…Particularly for C. albidus, bloodstream and CNS are the commonest sites of infection 8,12 . In addition, until now in Colombia, C. albidus has not been reported as a causative agent of disease and its isolation from environmental sources has been rather sporadic 13,14 .…”