Aktivitas Antibakteri Ekstrak Protein Crude Isi Saluran Pencernaan Ayam Broiler Yang Diberi Pakan Tambahan Pliek U

Abstract

ABSTRAKPenelitian ini bertujuan mengetahui pengaruh penambahan pliek u dalam ransum terhadap aktivitas antibakteri ekstrak protein crude isi saluran pencernaan ayam broiler. Penelitian ini dilakukan dengan mengisolasi ekstrak protein crude dari isi saluran pencernaan ayam broiler yang telah diberi pakan tambahan pliek u. Ayam broiler dibagi menjadi 4 kelompok perlakuan. Setiap perlakuan terdiri atas lima ulangan. Kelompok 1 sebagai kontrol, kelompok II, III, dan IV masing-masing diberi pakan dengan tambahan pliek u0,5;1; dan 2%. Rata-rata diameter zona hambat Basillus subtilis pada konsentrasi pliek u 0,5; 1, dan 2% masing-masing adalah 1,6±0,65; 3,48± ,03; 4,2±2,30 mm sedangkan pada kontrol tidak terukur (<1). Dari hasil penelitian dapat disimpulkan bahwa ekstrak protein crude dari saluran pencernaan ayam broiler yang diberi pakan tambahan pliek u dapat menghambat pertumbuhan bakteri Bacillus subtilis. ____________________________________________________________________________________________________________________ Kata kunci: aktivitas antibakteri, pliek u, ekstrak protein crude, saluran pencernaan 0.5, 1, ad 2% were 1.6±0.65, 3.48±0.30, 4.2±2.30 ABSTRACT The aim of the research was to knoe the effect of pliek u addition in feed of broiler on antibacterial activity of crude proten etract of broiler digestive tract contents. Broilers were divide into four treatment groups. Each treatment consisted of 5 replications. First group as a control group was fed without pliek u addition, group II, III, and IV were fed with the addition of 0.5, 1, and 2% pliek u, respectively. The average of inhibition zone diameter of Bacillus subtilis on pliek u concentration of

Effectiveness of antibacterial extract of coriander seeds (coriandrum sativum L.) against staphylococcus aureus

“…The most likely factor that causes the formation of inhibitory zones in the four samples with the same concentration is the difference in strains of bacteria between one sample and another because the test bacteria were taken from four different children. 10,11 A study conducted by Silva et al 8 using essential oils from coriandrum sativum L. was found to inhibit gram positive and gram negative bacteria, one of the gram-positive bacteria that can be inhibited is staphylococcus aureus SA08 with a minimum concentration of 0.4% , staphylococcus aureus ATCC 25923 with a minimum concentration of 0.2%, MRSA 08/08 with a minimum concentration of 0.2%, MRSA 08/08 with a minimum concentration of 0.8%. 8 Based on the minimum amount of concentration, the results of this study are not much different from previous studies with minimal concentrations that can inhibit staphylococcus aureus, which is a concentration of 0.8% which occurs in the second sample from bacteria so that it will inhibit bacterial cell growth, whereas for protease enzymes which are inhibited by linalool causing ability bacterial invasion of tissues becomes blocked so that bacteria staphylococcus aureus can no longer spread to host tissues because bacteria staphylococcus aureus can no longer reduce human fibronectin.…”