Aktivitas Antioksidan Krim Ekstrak Etil Asetat Kulit Jeruk Nipis (Citrus aurantifolia) Yang Ditetapkan Dengan Metode DPPH

Abstract

Lime peel (Citrus aurantifolia) contains flavonoids as antioxidants that prevent free radicals, and antioxidants are used to repair skin cells damaged by free radicals. Therefore, a research was conducted with the aim of formulating antioxidant cream preparations using lime peel extract. The extraction was carried out by maceration using ethanol 96% solvent, which was then fractionated with ethyl acetate as solvent. The ethyl acetate extract obtained was formulated into cream preparations with extract concentrations of 3%, 6%, and 9%. The formula obtained was tested for its physical and stability, and then for its antioxidant activity. The results showed that the physical properties of the cream preparation meet with requirements for the physical stability based on the organoleptic test parameters, homogeneity, pH and viscosity. Similarly, the antioxidant activity test of cream against the DPPH free radicals showed that formula 1 with an extract concentration of 3% gives an average IC50 value of 28.24 mg/ml; formula 2 with an extract concentration of 6% gives an average IC50 value of 22.97 mg/ml; and formula 3 with an extract concentration of 9% gives an average IC50 value of 14.80 mg/ml. Based on the results, it was concluded that the lime peel extract are formulated into a cream preparation with type M/A. Formula 3 with an extract concentration of 9% is the best formula with the greatest antioxidant activity.

Atsiri Oil Potential of Peel of Kaffir Lime, Lime, and Calamansi Orange as Antioxidant and Antibacteria

“…Kaffir lime essential oil has the potential to be used as an antibacterial agent in various applications. 2 (Nurisyah et al, 2020).…”

“…These bacteria are generally beneficial and necessary for a healthy human digestive tract. Escherichia coli is a pathogen that can cause diseases such as diarrhea and other intestinal tract diseases, transmission via contaminated water or food, or contact with animals or people (Nurisyah et al, 2020).…”

Formulation and characteristics of skin cream with the addition of essential oil blend

“…This value aligns with the requirements of SNI 16-4399-1996, which stipulates a range of 0.95-1.05. Generally, oxygenated terpene components have a higher specific gravity than non-oxygenated terpenes [15]. Effect of essential oil blend on specific gravity of the skin cream (values followed by different letters showed a significant difference in the DMRT0.05 and coefficient of variation = 0.0075%)…”

Review Artikel: Aktivitas Antioksidan Formulasi Sediaan Krim dari Berbagai Tanaman

“…Aktivitas antioksidan kulit jeruk nipis ini berkaitan dengan senyawa flavonoid, kumarin dan tanin. Formulasi yang digunakan pada sediaan krim ini melipuri ekstrak kulit jeruk nipis 9%, stearate acid 10%, setil alkohol 5%, lanolin 3%, tween 80 2,5%, span 80 2,5%, nipagin 0,18%, nipasol 0,02%, tokoferol 0,05% dan akuades ad 100 [30].…”