Aktivitas Cairan Kultur 12 Isolat Actinomycetes Terhadap Bakteri Resisten

Mulyadi, .; Sulistyani, Nanik

doi:10.12928/kesmas.v7i2.1043

Cited by 6 publications

(8 citation statements)

References 11 publications

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“…Fermentation on KC3-3 isolate was conducted using SNB (Starch Nitrate Broth) medium and then incubated for 14 days. According to [10], On the 14 th days of incubation, generally actinomycetes has entered the stationary phase. This is the phase of the bacteria do not show significant growth or the microorganism growth stop and the balance is present between the splitting cells and the dying cells.…”

Section: Fermentation and Antibacterial Activity Testmentioning

confidence: 99%

Actinomycetes of Orthosipon stamineus rhizosphere as producer of antibacterial compound against multidrug resistant bacteria

Rante

Yulianty

Usmar

et al. 2017

IOP Conf. Ser.: Mater. Sci. Eng.

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Abstract. The increasing case of antibiotic resistence has become an important problem to be faced in treating the infection diseases. The diversities of microbia, especially actinomycetes bacteria which originated from rizosphere soil of medicinal plant, has opened a chance for discovering the metabolites which can be used in solving the antibiotic resistant pathogenic bacteria problems. The aim of this research was to isolate the actinobacteria originated from medicinal plant rizosphere of Orthosipon stamineus as the producer of anti-multidrug resistances bacteria compounds. Three isolates of actinomycetes has been isolated from Orthosipon stamineus rhizosphere named KC3-1, KC3-2 and KC3-3. One isolate (KC3-3) showed big activity in inhibiting the test microbes by antagonistic test of actinomycetes isolates against Staphylococcus aureus and Eschericia coli antibiotic resistant bacteria. Furthermore, the KC3-3 isolate was fermented in Starch Nitrate Broth (SNB) medium for 14 days. The supernatant and the biomass of the fermentation yield were separated. The supernatant were extracted using ethyl acetate as the solvent and the biomass were extracted using methanol. The antibacterial activity test of ethyl acetate and methanol extract revealed that the extracts can inhibit the bacteria test up to 5% concentration. The ethyl acetate and methanol extracts can inhibit the bacteria test up to 5% concentration.

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Section: Fermentation and Antibacterial Activity Testmentioning

confidence: 99%

Actinomycetes of Orthosipon stamineus rhizosphere as producer of antibacterial compound against multidrug resistant bacteria

Rante

Yulianty

Usmar

et al. 2017

IOP Conf. Ser.: Mater. Sci. Eng.

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“…Sebanyak 100 μL, dimasukkan kedalam 1 mL BHI dan diinkubasi salama 4 sampai 8 jam pada suhu 37°C. Suspensi diencerkan menggunakan NaCl 0,9% hingga kekeruhan sesuai dengan standar Mc Farland (10⁸ CFU/mL) (13) .…”

Section: Bahan Dan Metodeunclassified

Penentuan Kurva Pertumbuhan dan Aktivitas Antibakteri dari Isolat Ekstrak Etil Asetat Bakteri (Te.325) terhadap Staphylococcus aureus dan Escherichia coli

Syarifuddin¹,

Wijayatri²,

Kurniawan³

et al. 2022

jifi

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Perkembangan kasus infeksi dan penggunaan antibiotik yang kurang tepat menimbulkan kasus resistensi antibiotik. Alternatif dalam mengatasi banyaknya antibiotik yang sudah resisten bakteri, yaitu dengan penemuan antibiotik baru. Proses penemuan antibiotik salah satunya dari mikroorganisme, yaitu dari bakteri. Proses eksplorasi penemuan antibiotik tersebut menggunakan pendekatan fase pertumbuhan bakteri, yaitu fase stasioner dimana bakteri memproduksi metabolit sekunder, salah satunya adalah senyawa antibiotik. Isolat Te.325 merupakan bakteri penghasil antibiotik tetapi belum diketahui fase pertumbuhannya yang dapat digunakan untuk melakukan pendekatan proses perolehan antibiotik. Tujuan penelitian ini untuk mendapatkan waktu fase pertumbuhan isolat Te.325 dan melakukan ekstraksi senyawa antibiotik dari isolat tersebut. Penentuan kurva pertumbuhan yaitu berdasarkan berat biomassa sel dan nilai absorbansi pada spektrofotometri UV/ Vis dari kultur yang disampling setiap hari selama 14 hari waktu inkubasi kultur. Hasil penelitian menunjukkan fase log/eksponensial pada hari ke-5 dan fase stasioner pada hari ke-9. Aktivitas antibakteri ekstrak etil asetat dilakukan pengujian menggunakan metode sumuran dengan konsentrasi ekstrak 40% yang menghasilkan rata-rata diameter zona hambat sebesar 8,04 mm terhadap bakteri uji Staphylococcus aureus dan 9,035 mm pada Escherichia coli. Ekstrak etilasetat Te.325 tergolong dalam potensi sedang.

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“…Setelah proses sentrifugasi selesai, terbentuk dua bagian lapisan, yakni supernatan (bagian atas) dan pellet (bagian bawah). Lapisan supernatan diambil sebagai sumber metabolit sekunder (Mulyadi & Sulistyani, 2013;Sharma et al, 2016).…”

Section: Pemisahan Cairan Kultur Actinomycetesunclassified

Aktivitas Antibakteri Actinomycetes Asal Desa Cempaka Kapuas Hulu Kalimantan Barat Terhadap Enteropatogenik Gastroenteritis

Abdullah

Almuhardi

Antoni

et al. 2020

Al-Kauniyah J. Biol.

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AbstrakAntibiotik yang digunakan secara tidak tepat dapat meningkatkan jumlah bakteri penyebab gastroenteritis yang resisten terhadap antibiotik. Beberapa kelompok mikroorganisme tanah diketahui memiliki potensi menghasilkan senyawa aktif untuk menghambat dan membunuh bakteri patogen, seperti Actinomycetes. Tujuan dari penelitian ini adalah untuk mengetahui potensi antibiotik Actinomycetes terhadap bakteri penyebab gastroenteritis yang diisolasi dari sawah tandus di Desa Cempaka Baru, Kabupaten Kapuas Hulu, Kalimantan Barat. Proses penelitian dilakukan dari isolasi Actinomycetes dari sawah tandus, karakterisasi, serta pengujian kemampuan antibiotik terhadap bakteri Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028 dan Staphylococcus aureus ATCC 25923 menggunakan metode sumuran pada media Mueller Hinton Agar. Hasil identifikasi berdasarkan karakteristik morfologi koloni, sel, dan biokimia diperoleh 1 isolat bakteri, yaitu genus Nocardia sp. ATS-4.1 yang mampu menghambat pertumbuhan bakteri-bakteri uji yang dibuktikan adanya rata-rata zona hambat yang terbentuk, yakni 14,51 mm; 16,16 mm; dan 11,10 mm. Hasil uji statistik Friedman diperoleh nilai Asymp. Sig 0,10>0,05, sehingga menunjukkan bahwa pemberian cairan kultur isolat Nocardia ATS-4.1 memberikan pengaruh yang tidak berbeda nyata antara hambatan terhadap bakteri E. coli, S. enterica, dan S. aureus. Hal ini dapat disimpulkan bahwa isolat Nocardia sp. ATS-4.1 berpotensi menghasilkan antibakteri yang setara terhadap bakteri E. coli, S. enterica, dan S. aureus penyebab gastroenteritis.Abstract Using antibiotics incorrectly increases the number of resistant bacteria to gastroenteritis. Soil microorganisms are known to have the potential to produce active compounds to inhibit and kill pathogenic bacteria, for example, Actinomycetes. The purpose of this study was to determine the potential of antibiotics Actinomycetes isolated from rice fields in Cempaka Baru, Kapuas Hulu Regency, West Kalimantan, against gastroenteritis bacteria. The research order was carried out by isolating Actinomycetes from barren rice fields, and then characterizing and testing the ability of antibiotics against Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028, and Staphylococcus aureus ATCC 25923 using the well method on Mueller Hinton Agar. The identification results based on morphological characteristics of colonies, cells and biochemistry showed that one of the bacteria was from genus Nocardia sp. ATS-4.1 which was able to inhibit the three test bacteria by the average inhibition zone 14.51 mm; 16.16 mm; and 11.10 mm, respectively. Friedman’s statistical test resulted in values asymp. sig 0.10>0.05 which showed that the isolate gave inhibition differences insignificantly among the bacteria. In conclusion, isolate Nocardia sp. ATS-4.1 able to produce equal antibacterial activity against bacteria E. coli, S. enterica and S. aureus caused gastroenteritis.

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Aktivitas Cairan Kultur 12 Isolat Actinomycetes Terhadap Bakteri Resisten

Abstract: ABSTRAK

Cited by 6 publications

References 11 publications

Actinomycetes of Orthosipon stamineus rhizosphere as producer of antibacterial compound against multidrug resistant bacteria

Actinomycetes of Orthosipon stamineus rhizosphere as producer of antibacterial compound against multidrug resistant bacteria

Penentuan Kurva Pertumbuhan dan Aktivitas Antibakteri dari Isolat Ekstrak Etil Asetat Bakteri (Te.325) terhadap Staphylococcus aureus dan Escherichia coli

Aktivitas Antibakteri Actinomycetes Asal Desa Cempaka Kapuas Hulu Kalimantan Barat Terhadap Enteropatogenik Gastroenteritis

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