AKTIVITAS ENZIM PENCERNAAN UDANG VANAME (Penaeus vannamei)  YANG DIBERIKAN PAKAN BERBAHAN BAKU TEPUNG Skeletonema costatum

Lestari, Dewi Putri

doi:10.29303/jp.v8i1.79

Cited by 2 publications

(3 citation statements)

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“…Amylase was measured based on Lestari (2018) and Al Gadri, Untung & Priyanto (2014) using the formula below. Sample, control and blank were checked in spectrophotometer at 540 nm.

EA = \frac{[A ‐ B] \times N NaOH \times 1000}{VE \times t}

…”

Section: Methodsmentioning

confidence: 99%

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Curative impacts of squid (Loligosp.) ink extract on haemocyte, digestive enzymes andCypAgene expression of Vaname Shrimp (Litopenaeus vannamei) against white faeces syndrome (WFS)

et al. 2020

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Right now, high economical value shrimp species is vaname shrimp (Litopenaeus vannamei). Salinity gives impact to shrimp live, immunity and health. Stress from salinity shock will reduce shrimp health and production because of pathogen attack. White faeces syndrome (WFS) is a shrimp disease which frequently occured in an intensive farm. Fighting against WFS has been done with several methods, among other things by using ink extract as metabolite product of squid (Loligo sp.) because of its antibacterial content. To solve the problem, combination of ink extract with salinity treatments to CypA gene expression THC, DHC and digestibility of vaname shrimp (L. vannamei) was investigated. The best treatment using squid ink extract was 27 ppt salinity for vaname shrimp (L. vannamei) against WFS, because it gave the highest SR (85.73%), increased protease until 378 × 10 −3 U/ml, amylase until 4.029 × 10 −3 unit/ml and lipase until 3.613 × 10 −3 unit/ml. and also non-specific immune, i.e. THC 11.89 × 10 6 cells/ml and DHC (hyaline was 39.09%, granular cell was 21.43% and semi-granular cell was 47.74%), while mutation (>1%) was happened in CypA gene.

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“…Amylase was measured based on Lestari (2018) and Al Gadri, Untung & Priyanto (2014) using the formula below. Sample, control and blank were checked in spectrophotometer at 540 nm.

EA = \frac{[A ‐ B] \times N NaOH \times 1000}{VE \times t}

…”

Section: Methodsmentioning

confidence: 99%

“…This proved that shrimp in treatment B gets a higher enzyme activity, linear with their growth. Based on Lestari (2018), vaname shrimp is omnivore and high protease activity related to shrimp digesting protein activity.…”

Section: Digestive Enzymesmentioning

confidence: 99%

Curative impacts of squid (Loligosp.) ink extract on haemocyte, digestive enzymes andCypAgene expression of Vaname Shrimp (Litopenaeus vannamei) against white faeces syndrome (WFS)

et al. 2020

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“…The previous study revealed that gel filtration chromatography was able to separate amylase enzyme from the digestion of Vannamei shrimp was 2.97 ± 0.11 units/mL (5). Other studies showed that amylase activity isolated from *Corresponding Author: hanifah_rahmi@uhamka.ac.id…”

Section: Introductionmentioning

confidence: 99%

Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method

Hariyanti

Rahmi

2021

J. Nat.

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Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;}

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AKTIVITAS ENZIM PENCERNAAN UDANG VANAME (Penaeus vannamei) YANG DIBERIKAN PAKAN BERBAHAN BAKU TEPUNG Skeletonema costatum

Cited by 2 publications

References 1 publication

Curative impacts of squid (Loligosp.) ink extract on haemocyte, digestive enzymes andCypAgene expression of Vaname Shrimp (Litopenaeus vannamei) against white faeces syndrome (WFS)

Curative impacts of squid (Loligosp.) ink extract on haemocyte, digestive enzymes andCypAgene expression of Vaname Shrimp (Litopenaeus vannamei) against white faeces syndrome (WFS)

Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method

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